Gene loci information

Transcript annotation

  • This transcript has been annotated as Ribonucleoside-diphosphate reductase subunit M2.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g6617 g6617.t16 TSS g6617.t16 17823834 17823834
chr_2 g6617 g6617.t16 isoform g6617.t16 17824622 17825702
chr_2 g6617 g6617.t16 exon g6617.t16.exon1 17824622 17825702
chr_2 g6617 g6617.t16 cds g6617.t16.CDS1 17824674 17825507
chr_2 g6617 g6617.t16 TTS g6617.t16 NA NA

Sequences

>g6617.t16 Gene=g6617 Length=1081
ACAGGCTGAAGCAAGCTTTTGGACTGTTGAAGAAGTTGATCTCTCTAAAGATATGAATGA
TTGGAATAATTTAAAAGATTGTGAACGACATTTTATATCGCATGTATTAGCTTTCTTTGC
GGCATCGGATGGAATAGTAAATGAAAATCTTGTTGAACGCTTTAGTCAAGAAGTTCAAGT
AACAGAAGCTCGCTGCTTTTATGGCTTTCAAATAGCTATGGAAAATGTTCATTCAGAAAT
GTATTCGTTACTCATTGAGACTTATGTTCGTGATTCTAAAGAACGTGAATTTCTCTTTAA
TGCTATTCAAACTTTGCCTTGTGTTAAAAAGAAAGCAGACTGGGCAATGAATTGGATATC
AAGCAAAAACGCAAATTTTGGCGAGAGAATCATTGCATTTGCATCTGTCGAGGGTATTTT
CTTCAGTGGTAGTTTTGCTGCAATATTTTGGCTAAAGAAACACGGACTTATACCTGGCTT
AACATTTTCGAATGAACTTATTTCACGCGATGAAGGCTTACATTGTGACTTTGCATGTCT
TATGTTTAAACATTTGGTTCAGAAGCCACCCAAAGAGAGAATAATTGAAATAATCTCAGA
GGCTGTTAAAATTGAACAAGAATTTTTAACTGATGCTCTACCTGTCAACATGTTGGGAAT
GAATTGTGATTTAATGTCACAATACATAGAATTTGTTGCTGATCGTTTATTAGTAGAGCT
AGGCGTCGAAAAAATTTATAATACAAAGAATCCATTCTCATTTATGGAATTCATTTCTTT
AGAAGGAAAAACTAATTTCTTTGAGAAAAAAGTTGGAGAATATCAAAAGTATGGAGTCAT
GTCAAGTCGAAAAGATCATGTTTTTACATTAGATGCTGATTTTTGATTTCACAATATTTT
TTTCATTTATAATTTTAGTATAATTCTGACTTTAATTTACAGATAAAATTACAATCTTCC
AAAATAATTATTTTAAAGGACATGCATAATTTGTTTACATAATGTATTATCATATTGTGT
GAAACCCGCCTTAATGCAATAAATTTATAATAGAAAGAACAAACAAATATGATTAATTAC
T

>g6617.t16 Gene=g6617 Length=277
MNDWNNLKDCERHFISHVLAFFAASDGIVNENLVERFSQEVQVTEARCFYGFQIAMENVH
SEMYSLLIETYVRDSKEREFLFNAIQTLPCVKKKADWAMNWISSKNANFGERIIAFASVE
GIFFSGSFAAIFWLKKHGLIPGLTFSNELISRDEGLHCDFACLMFKHLVQKPPKERIIEI
ISEAVKIEQEFLTDALPVNMLGMNCDLMSQYIEFVADRLLVELGVEKIYNTKNPFSFMEF
ISLEGKTNFFEKKVGEYQKYGVMSSRKDHVFTLDADF

Protein features from InterProScan

Transcript Database ID Name Start End E.value
9 g6617.t16 CDD cd01049 RNRR2 1 243 6.103E-114
5 g6617.t16 Gene3D G3DSA:1.10.620.20 Ribonucleotide Reductase 1 259 1.2E-117
2 g6617.t16 PANTHER PTHR23409:SF20 RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SUBUNIT M2 2 277 1.6E-162
3 g6617.t16 PANTHER PTHR23409 RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SMALL CHAIN 2 277 1.6E-162
1 g6617.t16 Pfam PF00268 Ribonucleotide reductase, small chain 1 234 1.3E-99
6 g6617.t16 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 1 112 -
8 g6617.t16 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 113 134 -
7 g6617.t16 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 135 277 -
11 g6617.t16 ProSitePatterns PS00368 Ribonucleotide reductase small subunit signature. 56 72 -
4 g6617.t16 SUPERFAMILY SSF47240 Ferritin-like 2 259 2.37E-101
10 g6617.t16 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 112 134 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0009263 deoxyribonucleotide biosynthetic process BP
GO:0055114 NA NA
GO:0016491 oxidoreductase activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values