Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Nascent polypeptide-associated complex subunit alpha.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g6773 g6773.t5 isoform g6773.t5 18557877 18559262
chr_2 g6773 g6773.t5 exon g6773.t5.exon1 18557877 18558092
chr_2 g6773 g6773.t5 TTS g6773.t5 18557899 18557899
chr_2 g6773 g6773.t5 cds g6773.t5.CDS1 18558066 18558092
chr_2 g6773 g6773.t5 exon g6773.t5.exon2 18558215 18558762
chr_2 g6773 g6773.t5 cds g6773.t5.CDS2 18558215 18558751
chr_2 g6773 g6773.t5 exon g6773.t5.exon3 18559213 18559262
chr_2 g6773 g6773.t5 TSS g6773.t5 18559262 18559262

Sequences

>g6773.t5 Gene=g6773 Length=814
CTTTTTCGCTCTCACATTTGCAAAGAAAACAAAGTAATTTTTTAAAAAACAATCTTTTAC
GATGCCAGAATTAACTGAAATCAAAGCTGAAAATCCTGCAGCAGCTACAGAAGCAGAACC
AAAATTAACAGATGACGTCAGCGATACTGAGTCAGAAGAATCAATTCCAGAATTGGAAGA
TGCCACCAATGAAGCTCAATTAGGTACTGGTGGTTCAACTGGACTTCAATCAGATATGGT
TTCGAAAGCAAAACAATCTCGAGGAGAGAAAAAAGCACGTAAGATTATGTCAAAGTTGGG
ATTGAAACCAGTCACTGGAGTTACAAGAGTTACAATTCGCAAATCAAAGAACATTCTCTT
TGTAATCAATAATCCGGATGTTTACAAGAATCCGCATAGTGATACATACATCATTTTTGG
TGAGGCAAAAATAGAAGACTTGTCTCAACAAACGCAAGTTGCAGCAGCTGAAAAATTCAA
GGCACCCGAAACAGTTCCTACTGATACCACTTCAGCCAATGTTGTTGCTCCGATTGCTGA
AGAAGACGAGGAAGAAGTTGATGATACTGGGGTCGATGAAAAGGATATTGACCTTGTTAT
CAATTGTTTCATCTACGTTTCTTGATTTCTCATAAATTATTTGGCATTCGTTCAACTACA
AGATAATAAAGTTATAACTATAACTTCTTAATCATATAAACCGAAGAAAATTTTACTTTC
ATACATTTAAATTGTACGTTTTTCTTTCTTTTGCATTGTTTCAAAATAAACAGAAATGAG
TATGATGAAATTTAATTCTTGATACTTTATTGAA

>g6773.t5 Gene=g6773 Length=187
MPELTEIKAENPAAATEAEPKLTDDVSDTESEESIPELEDATNEAQLGTGGSTGLQSDMV
SKAKQSRGEKKARKIMSKLGLKPVTGVTRVTIRKSKNILFVINNPDVYKNPHSDTYIIFG
EAKIEDLSQQTQVAAAEKFKAPETVPTDTTSANVVAPIAEEDEEEVDDTGVDEKDIDLVI
NCFIYVS

Protein features from InterProScan

Transcript Database ID Name Start End E.value
7 g6773.t5 Gene3D G3DSA:2.20.70.30 - 79 132 9.5E-33
6 g6773.t5 MobiDBLite mobidb-lite consensus disorder prediction 1 74 -
5 g6773.t5 MobiDBLite mobidb-lite consensus disorder prediction 45 62 -
2 g6773.t5 PANTHER PTHR21713 NASCENT POLYPEPTIDE ASSOCIATED COMPLEX ALPHA SUBUNIT-RELATED 30 179 1.4E-62
3 g6773.t5 PANTHER PTHR21713:SF1 NAC-ALPHA DOMAIN-CONTAINING PROTEIN 1 30 179 1.4E-62
9 g6773.t5 PIRSF PIRSF015901 NAC_alpha 27 182 4.5E-50
1 g6773.t5 Pfam PF01849 NAC domain 69 124 2.2E-23
8 g6773.t5 ProSiteProfiles PS51151 NAC A/B domain profile. 66 131 18.628
4 g6773.t5 SMART SM01407 NAC_2 69 125 1.5E-28

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005854 nascent polypeptide-associated complex CC

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values