General information for g6773.t7
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Nascent polypeptide-associated complex subunit alpha.
Parent gene
- The parent gene g6773 is located on chr_2: 18558119..18564193 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6773 | g6773.t7 | TTS | g6773.t7 | 18557899 | 18557899 |
| chr_2 | g6773 | g6773.t7 | isoform | g6773.t7 | 18558119 | 18559262 |
| chr_2 | g6773 | g6773.t7 | exon | g6773.t7.exon1 | 18558119 | 18558754 |
| chr_2 | g6773 | g6773.t7 | cds | g6773.t7.CDS1 | 18558119 | 18558751 |
| chr_2 | g6773 | g6773.t7 | exon | g6773.t7.exon2 | 18559213 | 18559262 |
| chr_2 | g6773 | g6773.t7 | TSS | g6773.t7 | 18559262 | 18559262 |
Sequences
>g6773.t7 Gene=g6773 Length=686
CTTTTTCGCTCTCACATTTGCAAAGAAAACAAAGTAATTTTTTAAAAAACACGATGCCAG
AATTAACTGAAATCAAAGCTGAAAATCCTGCAGCAGCTACAGAAGCAGAACCAAAATTAA
CAGATGACGTCAGCGATACTGAGTCAGAAGAATCAATTCCAGAATTGGAAGATGCCACCA
ATGAAGCTCAATTAGGTACTGGTGGTTCAACTGGACTTCAATCAGATATGGTTTCGAAAG
CAAAACAATCTCGAGGAGAGAAAAAAGCACGTAAGATTATGTCAAAGTTGGGATTGAAAC
CAGTCACTGGAGTTACAAGAGTTACAATTCGCAAATCAAAGAACATTCTCTTTGTAATCA
ATAATCCGGATGTTTACAAGAATCCGCATAGTGATACATACATCATTTTTGGTGAGGCAA
AAATAGAAGACTTGTCTCAACAAACGCAAGTTGCAGCAGCTGAAAAATTCAAGGCACCCG
AAACAGTTCCTACTGATACCACTTCAGCCAATGTTGTTGCTCCGATTGCTGAAGAAGACG
AGGAAGAAGTTGATGATACTGGGGTCGATGAAAAGGATATTGACCTTGTTGTGTCACAAG
CAAATGTCCCTCGTGCTAAAGCTATCCGCGCCCTTAAGAACAACAATAATGATATAGTCA
ACGCAATCATGGAACTAACGATGTAA
>g6773.t7 Gene=g6773 Length=210
MPELTEIKAENPAAATEAEPKLTDDVSDTESEESIPELEDATNEAQLGTGGSTGLQSDMV
SKAKQSRGEKKARKIMSKLGLKPVTGVTRVTIRKSKNILFVINNPDVYKNPHSDTYIIFG
EAKIEDLSQQTQVAAAEKFKAPETVPTDTTSANVVAPIAEEDEEEVDDTGVDEKDIDLVV
SQANVPRAKAIRALKNNNNDIVNAIMELTM
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g6773.t7 | CDD | cd14358 | UBA_NAC_euk | 173 | 209 | 5.22315E-17 |
| 6 | g6773.t7 | Gene3D | G3DSA:2.20.70.30 | - | 79 | 132 | 1.2E-32 |
| 5 | g6773.t7 | Gene3D | G3DSA:1.10.8.10 | DNA helicase RuvA subunit | 170 | 209 | 4.7E-14 |
| 11 | g6773.t7 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 74 | - |
| 10 | g6773.t7 | MobiDBLite | mobidb-lite | consensus disorder prediction | 45 | 62 | - |
| 3 | g6773.t7 | PANTHER | PTHR21713 | NASCENT POLYPEPTIDE ASSOCIATED COMPLEX ALPHA SUBUNIT-RELATED | 30 | 210 | 5.0E-87 |
| 4 | g6773.t7 | PANTHER | PTHR21713:SF1 | NAC-ALPHA DOMAIN-CONTAINING PROTEIN 1 | 30 | 210 | 5.0E-87 |
| 7 | g6773.t7 | PIRSF | PIRSF015901 | NAC_alpha | 27 | 210 | 2.7E-70 |
| 1 | g6773.t7 | Pfam | PF01849 | NAC domain | 69 | 124 | 2.8E-23 |
| 2 | g6773.t7 | Pfam | PF19026 | HYPK UBA domain | 170 | 209 | 5.8E-11 |
| 12 | g6773.t7 | ProSiteProfiles | PS51151 | NAC A/B domain profile. | 66 | 131 | 18.628 |
| 9 | g6773.t7 | SMART | SM01407 | NAC_2 | 69 | 125 | 1.5E-28 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005854 | nascent polypeptide-associated complex | CC |
KEGG
Orthology
- This transcript has been annotated with K03626 (nascent polypeptide-associated complex subunit alpha)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
