General information for g6936.t21
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Triosephosphate isomerase.
Parent gene
- The parent gene g6936 is located on chr_2: 20044463..20045782 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6936 | g6936.t21 | isoform | g6936.t21 | 20044082 | 20045161 |
| chr_2 | g6936 | g6936.t21 | exon | g6936.t21.exon1 | 20044082 | 20044117 |
| chr_2 | g6936 | g6936.t21 | exon | g6936.t21.exon2 | 20044235 | 20044380 |
| chr_2 | g6936 | g6936.t21 | cds | g6936.t21.CDS1 | 20044266 | 20044380 |
| chr_2 | g6936 | g6936.t21 | exon | g6936.t21.exon3 | 20044470 | 20044540 |
| chr_2 | g6936 | g6936.t21 | cds | g6936.t21.CDS2 | 20044470 | 20044540 |
| chr_2 | g6936 | g6936.t21 | exon | g6936.t21.exon4 | 20044607 | 20045161 |
| chr_2 | g6936 | g6936.t21 | cds | g6936.t21.CDS3 | 20044607 | 20045161 |
| chr_2 | g6936 | g6936.t21 | TTS | g6936.t21 | 20045299 | 20045299 |
| chr_2 | g6936 | g6936.t21 | TSS | g6936.t21 | NA | NA |
Sequences
>g6936.t21 Gene=g6936 Length=808
ATTGAAAGGTCAACAGATTTTCGCAAATTTCATCCGTTGATTAAATAGCAAAAAAGACAG
CAACAAAATGGGTCGCAAATTTTGTGTTGGTGGAAATTGGAAAATGAATGGATCTAAGGA
TTCTATCACAGAATTGGCCAAAATCCTTTCTGCTGGACCATTAGATCCAAATACTGAAGT
GATTGGATGCCCAAGCGTTTATATCTCATTCGCTCGAGGACTTCTTCCTTCCTCAATTGG
TGTTGCTGGCCAGAATTGCTATAAAGTCAAATCAGGAGCTTTCACAGGAGAAATCGCACC
ATCAATGTTAAAAGATGTTGGTGCTGATTGGGTTATTCTTGGACACTCAGAGAGACGTGC
AATTTTCGGCGAAAGTGATGAATTAATCGCAGATAAAGTTGAATTTGCCCTTTCAGAAGG
ATTGAAAGTCATTGCTTGCATTGGTGAAACTCTTCAAGAACGTGAGGCTGGTCAAACTGA
AGCAGTATGCTTCCGTCAAACGAAGGCAATAGCTTCTGTTGTCAAAGACTGGTCAAATGT
TGTTATCGCTTATGAACCGGTTTGGGCAATTGGAACTGGCAAAACTGCAACTCCAGCTCA
AGCCCAAGAGGTTCATGCTGCTCTCCGTAAATGGTTCGCCGAGAACGTTTCTCAAGCCGT
TTCTGATTCAATTCGTATTCAATATGGTGGCTCAGTTACTGCTGCTAATGCTCGTGAATT
AGCTTCACAACCAGATATCGATGGATTTTTAGTTGGAGGTGCATCATTAAAACCCGAATT
CGTACAAATTGTCAATGCTAGGCAGTAA
>g6936.t21 Gene=g6936 Length=246
MGRKFCVGGNWKMNGSKDSITELAKILSAGPLDPNTEVIGCPSVYISFARGLLPSSIGVA
GQNCYKVKSGAFTGEIAPSMLKDVGADWVILGHSERRAIFGESDELIADKVEFALSEGLK
VIACIGETLQEREAGQTEAVCFRQTKAIASVVKDWSNVVIAYEPVWAIGTGKTATPAQAQ
EVHAALRKWFAENVSQAVSDSIRIQYGGSVTAANARELASQPDIDGFLVGGASLKPEFVQ
IVNARQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g6936.t21 | CDD | cd00311 | TIM | 5 | 243 | 1.1221E-131 |
| 6 | g6936.t21 | Gene3D | G3DSA:3.20.20.70 | Aldolase class I | 1 | 246 | 3.5E-104 |
| 4 | g6936.t21 | Hamap | MF_00147_B | Triosephosphate isomerase [tpiA]. | 3 | 244 | 58.931541 |
| 2 | g6936.t21 | PANTHER | PTHR21139:SF17 | TRIOSEPHOSPHATE ISOMERASE A | 3 | 245 | 6.9E-114 |
| 3 | g6936.t21 | PANTHER | PTHR21139 | TRIOSEPHOSPHATE ISOMERASE | 3 | 245 | 6.9E-114 |
| 1 | g6936.t21 | Pfam | PF00121 | Triosephosphate isomerase | 6 | 242 | 2.2E-87 |
| 8 | g6936.t21 | ProSitePatterns | PS00171 | Triosephosphate isomerase active site. | 161 | 171 | - |
| 10 | g6936.t21 | ProSiteProfiles | PS51440 | Triosephosphate isomerase (TIM) family profile. | 4 | 244 | 81.886 |
| 5 | g6936.t21 | SUPERFAMILY | SSF51351 | Triosephosphate isomerase (TIM) | 3 | 244 | 4.45E-94 |
| 9 | g6936.t21 | TIGRFAM | TIGR00419 | tim: triose-phosphate isomerase | 7 | 237 | 1.8E-72 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006096 | glycolytic process | BP |
| GO:0004807 | triose-phosphate isomerase activity | MF |
| GO:0003824 | catalytic activity | MF |
KEGG
Orthology
- This transcript has been annotated with K01803 (triosephosphate isomerase (TIM))
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
