General information for g7101.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as NAD kinase 2, mitochondrial.
Parent gene
- The parent gene g7101 is located on chr_2: 21189768..21192179 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7101 | g7101.t1 | TTS | g7101.t1 | 21188505 | 21188505 |
| chr_2 | g7101 | g7101.t1 | isoform | g7101.t1 | 21188736 | 21192179 |
| chr_2 | g7101 | g7101.t1 | exon | g7101.t1.exon1 | 21188736 | 21188842 |
| chr_2 | g7101 | g7101.t1 | cds | g7101.t1.CDS1 | 21188736 | 21188842 |
| chr_2 | g7101 | g7101.t1 | exon | g7101.t1.exon2 | 21188916 | 21189041 |
| chr_2 | g7101 | g7101.t1 | cds | g7101.t1.CDS2 | 21188916 | 21189041 |
| chr_2 | g7101 | g7101.t1 | exon | g7101.t1.exon3 | 21189108 | 21189378 |
| chr_2 | g7101 | g7101.t1 | cds | g7101.t1.CDS3 | 21189108 | 21189378 |
| chr_2 | g7101 | g7101.t1 | exon | g7101.t1.exon4 | 21189770 | 21190578 |
| chr_2 | g7101 | g7101.t1 | cds | g7101.t1.CDS4 | 21189770 | 21190578 |
| chr_2 | g7101 | g7101.t1 | exon | g7101.t1.exon5 | 21192140 | 21192179 |
| chr_2 | g7101 | g7101.t1 | cds | g7101.t1.CDS5 | 21192140 | 21192179 |
| chr_2 | g7101 | g7101.t1 | TSS | g7101.t1 | 21192404 | 21192404 |
Sequences
>g7101.t1 Gene=g7101 Length=1353
ATGCAAAGATCTAATAAGTTAGCAACAGTTGTCCTTGCAGGTTTTCGAAAGCATGCAAAA
AAGACAGCAACACATTTTTTAAAGGCAAAGTACCAACCAACTTATCACATTGCCAAACAT
CAGTCGACGTATGTGAATAATCATTGGTTCCATTCTCATGCTAAGAATAATAAATTGCGC
ATCAAGAAAGTTCTTATAGTGACAAAATTAACTCGATATGAATTTGAGAAAATTCGACAC
GCTGAATTAAATGATGCTGAACTTGAAGAAAAGATTAAATATCGTGGCACAGATTATGAT
GCACTCATGTATTATCATAATCTTCATAAGGCAGTTGAACGTAAAGTAGTTGACAGTTTT
AGTCAAATGGGCGTAGAAGTCAAGGTTGTTAATCGTTTGACAATCAACAAAGAGTTACTT
CAATGGACTGATTTAATTGTGCCAGTTGGTGGAGATGGAACTTTTCTTCTTGCCGCTTCA
CGTGCAACTCCCTTCTTTATAGAAAATTCCGTCCCTATCGTAGGTTTTAATTCTGATCCA
CAACGCTCGGAAGGCAGACTTATGTTGCCAAAACAGTATACGAGTGATGTAAATGATGCT
GTAAAGAAAATAATTAAAGGAGAATTTGAATGGATGCATAGATCACGTATCAGAATAACT
CTATTGAAATTTAATGGTGAAACACCAATACCTATAGATCTTCATGAATTTAATCCTGCG
CCTGTAGAGCACAAAGAACTTTTTGTGTCTGAACCTTCGTTACTGGATCAAATTAATGGA
GGAGCGTATTACCCAGGAAGAAAGAATAAAGCAACAAAACGCATATTGCCGTACTTGGCG
CTTAATGAGGTTTTCATTGGAGAAACCTTATCAGCCCGAGTTTCACATCTACACATTCGT
CCAAGTACAACTCAAAAAATTACAAAAACAAAAAGTTCAGGCTTGTGTGTATCAACAGGA
ACAGGATCGACAAGCTGGCATACATCTATCAATCGTCTATCTAATAAAAATGTTGAGGAT
TTACTACAGCTTCTAAAGAAAAACAATAAAGTAGATTTGAATGGCGTTGATGCTGGTGAG
ATATGTGAGGAATATAATCGTCAACTTGTTTTTGAGCCTGACGACCCGAGATTATGCTAC
TCAATTAGAGAACAAATTTGTGTTGGTGTTTGGCCAAATCCAAAAGGATTTGAATCACGT
GATTTCGCGCAAAATTTATATGTAAAAAGTAGATGCTTCGATGCAAGTCTGGTGATTGAT
GGTACTGTATCATATAAATTTAATGATGGTACGAAGGCGCTCTTAGAAGTATTACCTGAA
GACGCTTTATTGACTATGAAGATAGAAGATTAA
>g7101.t1 Gene=g7101 Length=450
MQRSNKLATVVLAGFRKHAKKTATHFLKAKYQPTYHIAKHQSTYVNNHWFHSHAKNNKLR
IKKVLIVTKLTRYEFEKIRHAELNDAELEEKIKYRGTDYDALMYYHNLHKAVERKVVDSF
SQMGVEVKVVNRLTINKELLQWTDLIVPVGGDGTFLLAASRATPFFIENSVPIVGFNSDP
QRSEGRLMLPKQYTSDVNDAVKKIIKGEFEWMHRSRIRITLLKFNGETPIPIDLHEFNPA
PVEHKELFVSEPSLLDQINGGAYYPGRKNKATKRILPYLALNEVFIGETLSARVSHLHIR
PSTTQKITKTKSSGLCVSTGTGSTSWHTSINRLSNKNVEDLLQLLKKNNKVDLNGVDAGE
ICEEYNRQLVFEPDDPRLCYSIREQICVGVWPNPKGFESRDFAQNLYVKSRCFDASLVID
GTVSYKFNDGTKALLEVLPEDALLTMKIED
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g7101.t1 | Gene3D | G3DSA:3.40.50.10330 | - | 60 | 229 | 3.3E-9 |
| 6 | g7101.t1 | Gene3D | G3DSA:2.60.200.30 | - | 256 | 331 | 3.1E-7 |
| 2 | g7101.t1 | PANTHER | PTHR13158 | - | 29 | 449 | 3.9E-128 |
| 3 | g7101.t1 | PANTHER | PTHR13158:SF5 | NAD KINASE 2, MITOCHONDRIAL | 29 | 449 | 3.9E-128 |
| 12 | g7101.t1 | PIRSF | PIRSF017565 | Kin_ATP-NAD_euk | 35 | 449 | 2.2E-161 |
| 1 | g7101.t1 | Pfam | PF01513 | ATP-NAD kinase | 112 | 326 | 5.2E-11 |
| 8 | g7101.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 19 | - |
| 9 | g7101.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 6 | - |
| 10 | g7101.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 7 | 15 | - |
| 11 | g7101.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 19 | - |
| 7 | g7101.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 20 | 450 | - |
| 4 | g7101.t1 | SUPERFAMILY | SSF111331 | NAD kinase/diacylglycerol kinase-like | 98 | 331 | 3.4E-27 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0003951 | NAD+ kinase activity | MF |
| GO:0006741 | NADP biosynthetic process | BP |
| GO:0019674 | NAD metabolic process | BP |
KEGG
Orthology
- This transcript has been annotated with K00858 (NAD+ kinase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
