General information for g7450.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Phenoloxidase-activating factor 3.
Parent gene
- The parent gene g7450 is located on chr_2: 23789976..23791458 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7450 | g7450.t1 | TSS | g7450.t1 | 23789913 | 23789913 |
| chr_2 | g7450 | g7450.t1 | isoform | g7450.t1 | 23789976 | 23791458 |
| chr_2 | g7450 | g7450.t1 | exon | g7450.t1.exon1 | 23789976 | 23790485 |
| chr_2 | g7450 | g7450.t1 | cds | g7450.t1.CDS1 | 23789976 | 23790485 |
| chr_2 | g7450 | g7450.t1 | exon | g7450.t1.exon2 | 23790773 | 23790832 |
| chr_2 | g7450 | g7450.t1 | cds | g7450.t1.CDS2 | 23790773 | 23790832 |
| chr_2 | g7450 | g7450.t1 | exon | g7450.t1.exon3 | 23790901 | 23791267 |
| chr_2 | g7450 | g7450.t1 | cds | g7450.t1.CDS3 | 23790901 | 23791267 |
| chr_2 | g7450 | g7450.t1 | exon | g7450.t1.exon4 | 23791325 | 23791458 |
| chr_2 | g7450 | g7450.t1 | cds | g7450.t1.CDS4 | 23791325 | 23791458 |
| chr_2 | g7450 | g7450.t1 | TTS | g7450.t1 | 23791549 | 23791549 |
Sequences
>g7450.t1 Gene=g7450 Length=1071
ATGTTGAAAGCAATTTTAATAATTTTGTTAGCGACTCGTATCGCAAATTGTCAAGAAACA
ATGTTTTTGCGAGTATCTGATATAGACAACTCAGTTTTGGCAGATGGTGAGCAATGTTCA
TTAAGTGGTGGCCGTCAAGGTATTTGCAAAGAAATTACCCAATGTGATTCAGTGAAAAAA
CTTTTAGTTACCAAAAGAACATCAGAAATTGTTCGTTGCTCATTTCATGGAATAAAACCG
CTTGTTTGTTGTCCACAATCACAAAAATTTATTAAAGCATTATGCGAAAATTTAAAACCA
ATTCCACTTGGTCTGGATTTCAAAATTACAAATGGGTTAAAGGCTGATGTAGGAGAATTT
CCTTATCAAGCAGCATTAGGCAGAAAAAACCTTGAAAATTTTTTAGAATTCAACTGTGGA
GGATCATTGATTGCTGACGATATCGTATTGACAGCTGCACATTGTTTAAATAAAATTAAA
GAAGCTGCTACAATGGTTCGATTGGGAAGAACTTCACTAAATCTCACTGATGAATTTGAT
GATTCAACTGCAGAAGATATTGACATTGAAACATTCAAACTTCATCCAAAATACAACAGT
AGATCAAGACAAAATGATATTGCTTTAATCAAACTTAAGAGACCGGCAAAACTAAGTGAC
AATATAAAACCAATTTGTTTATCAACAGATGATAAAAATATTCCAACAAATTTGACAATT
ACTGGTTATGGATCAACTCAGCCTGGAACAAGTACAAAAAGTTCACTCTCAGATTGGCTT
TTAAAAGGAACAGTTCAATCATATCCAAATGATGAATGCACTAAAATGTATAAATCACTC
GGCAAAGATCTCATTAACAGTCAATATTGTGCTAATAGTGTTTCTGGAGTTGATGCATGC
CAAGGCGATTCTGGCGGTGCATTAAATTATAAAAAAGATGGCTATTATTTTCAATATGGT
GTGATTTCATATGGCGCCGGATGTGGTTCACCGTTTCCAGCGATTTATACGAAAGTAAAT
CAATATCTCGACTGGATAGGCGATGAAATGTTGAAATTTGATGGCCAATAG
>g7450.t1 Gene=g7450 Length=356
MLKAILIILLATRIANCQETMFLRVSDIDNSVLADGEQCSLSGGRQGICKEITQCDSVKK
LLVTKRTSEIVRCSFHGIKPLVCCPQSQKFIKALCENLKPIPLGLDFKITNGLKADVGEF
PYQAALGRKNLENFLEFNCGGSLIADDIVLTAAHCLNKIKEAATMVRLGRTSLNLTDEFD
DSTAEDIDIETFKLHPKYNSRSRQNDIALIKLKRPAKLSDNIKPICLSTDDKNIPTNLTI
TGYGSTQPGTSTKSSLSDWLLKGTVQSYPNDECTKMYKSLGKDLINSQYCANSVSGVDAC
QGDSGGALNYKKDGYYFQYGVISYGAGCGSPFPAIYTKVNQYLDWIGDEMLKFDGQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 18 | g7450.t1 | CDD | cd00190 | Tryp_SPc | 109 | 346 | 9.28642E-82 |
| 11 | g7450.t1 | Gene3D | G3DSA:2.20.20.90 | - | 29 | 85 | 3.4E-6 |
| 12 | g7450.t1 | Gene3D | G3DSA:2.40.10.10 | - | 104 | 351 | 1.7E-68 |
| 3 | g7450.t1 | PANTHER | PTHR24251:SF13 | AGAP010547-PA | 8 | 236 | 2.3E-71 |
| 5 | g7450.t1 | PANTHER | PTHR24251 | OVOCHYMASE-RELATED | 8 | 236 | 2.3E-71 |
| 2 | g7450.t1 | PANTHER | PTHR24251:SF13 | AGAP010547-PA | 114 | 350 | 2.3E-71 |
| 4 | g7450.t1 | PANTHER | PTHR24251 | OVOCHYMASE-RELATED | 114 | 350 | 2.3E-71 |
| 6 | g7450.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 140 | 155 | 3.6E-14 |
| 8 | g7450.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 202 | 216 | 3.6E-14 |
| 7 | g7450.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 297 | 309 | 3.6E-14 |
| 1 | g7450.t1 | Pfam | PF00089 | Trypsin | 109 | 346 | 4.8E-54 |
| 14 | g7450.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 17 | - |
| 15 | g7450.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 16 | g7450.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 12 | - |
| 17 | g7450.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 13 | 17 | - |
| 13 | g7450.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 18 | 356 | - |
| 21 | g7450.t1 | ProSitePatterns | PS00134 | Serine proteases, trypsin family, histidine active site. | 150 | 155 | - |
| 22 | g7450.t1 | ProSitePatterns | PS00135 | Serine proteases, trypsin family, serine active site. | 298 | 309 | - |
| 24 | g7450.t1 | ProSiteProfiles | PS51888 | Clip domain profile. | 38 | 84 | 10.557 |
| 23 | g7450.t1 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 109 | 351 | 28.5 |
| 20 | g7450.t1 | SMART | SM00680 | clip | 39 | 85 | 2.5E-5 |
| 19 | g7450.t1 | SMART | SM00020 | trypsin_2 | 108 | 346 | 1.4E-73 |
| 9 | g7450.t1 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 79 | 350 | 6.2E-73 |
| 10 | g7450.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 17 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed