General information for g7662.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Gamma-glutamyl hydrolase.
Parent gene
- The parent gene g7662 is located on chr_2: 25155023..25155938 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7662 | g7662.t2 | TSS | g7662.t2 | 25153356 | 25153356 |
| chr_2 | g7662 | g7662.t2 | isoform | g7662.t2 | 25153398 | 25155529 |
| chr_2 | g7662 | g7662.t2 | exon | g7662.t2.exon1 | 25153398 | 25153660 |
| chr_2 | g7662 | g7662.t2 | cds | g7662.t2.CDS1 | 25153398 | 25153660 |
| chr_2 | g7662 | g7662.t2 | exon | g7662.t2.exon2 | 25154494 | 25154544 |
| chr_2 | g7662 | g7662.t2 | cds | g7662.t2.CDS2 | 25154494 | 25154544 |
| chr_2 | g7662 | g7662.t2 | exon | g7662.t2.exon3 | 25154607 | 25154943 |
| chr_2 | g7662 | g7662.t2 | cds | g7662.t2.CDS3 | 25154607 | 25154943 |
| chr_2 | g7662 | g7662.t2 | exon | g7662.t2.exon4 | 25155023 | 25155529 |
| chr_2 | g7662 | g7662.t2 | cds | g7662.t2.CDS4 | 25155023 | 25155472 |
| chr_2 | g7662 | g7662.t2 | TTS | g7662.t2 | 25155932 | 25155932 |
Sequences
>g7662.t2 Gene=g7662 Length=1158
ATGTCTTTCAAATTAAATTTATCTCTACTTATTGTGCTGTTTTGCACGCACAACTGCATT
TATTTGATAAATGGACGTTCAATCATCAGCACAGACAGCATTTTTAAAACACCACAAATT
AATAATAATCCAATTATCGGCATTTTAGCTGAGGAGTTGTTGTATTCGCTTGAAGACAAA
TATCCTGACCAGTATCATAGCTACATAGCCTCATCGTATGTGAAGTTTGTTGAAGGAGGT
GGTGCACGAGTTGTGCCCGTTTGGATAGGAAAACCACGTGAATATTATGAAGATATCATG
TCAAAACTCAACGGAATTCTCTTTCCCGGTGGTTCACTGTGGTTTAATCAAACAAACGGC
TACTCGGAAGCGGGAATGCATATTTATGAAATTGCAGAAAAGATGAATGATGAAGGCGAC
TATTTTCCTATCTGGGGAACATGTTTAGGTTTCGAATTGCTCACATATCTATCGGCAAAA
GGTGAAGAACATAGAGTTGATTGTTCATCGAATAGTCAATCTTTGCCGCTTATCTTTAAA
GATGGTTTTCATGAAAGTCGAATGTTTAAAAATGCTCCGTCACACGTTATCGAAATTCTT
CGCACTGAACCCGTCACATCAAACTTCCATCAATTTTGTGTAACAGAAAAGAATTTGACA
GATTTTCATATCGATAAGGAATGGCATGTAATATCAGTGAATGATGATTGGAATGGTTTG
GAATTTATATCAACAATTGAGAATTATCGTTATCCATTCTATGGCATTCAATTTCATCCC
GAAAAGAATTTATATGAATGGGTGCGTAATAAAAATATTTCGCACACTACAAATGCTGTG
ATAGCATCGCAATATTTTGCTGAATTTTTTGTCAGTGAAGCACGTAAAAACGATCATCAT
TTTGCTGATGCAAAAACTGAGGATCTGTACGTTATTTACAATTACCCAGAAACTTTTACT
GGTGCTAAAGGATCGTCCTTTGAACAATGTTACATGTTTCCTGAAGACGTTGATTATGTC
AAAACTAATAATGCTCATGGTACTTATGTTAGCATTATTGTTATATTATTAGCTTTATCA
GTATTAAAAGCAATTGAATAAAACTAAAACTTTTTTATTTCTTCTTATCTTACAGTGCAA
GATGACACGAACGTTTAG
>g7662.t2 Gene=g7662 Length=366
MSFKLNLSLLIVLFCTHNCIYLINGRSIISTDSIFKTPQINNNPIIGILAEELLYSLEDK
YPDQYHSYIASSYVKFVEGGGARVVPVWIGKPREYYEDIMSKLNGILFPGGSLWFNQTNG
YSEAGMHIYEIAEKMNDEGDYFPIWGTCLGFELLTYLSAKGEEHRVDCSSNSQSLPLIFK
DGFHESRMFKNAPSHVIEILRTEPVTSNFHQFCVTEKNLTDFHIDKEWHVISVNDDWNGL
EFISTIENYRYPFYGIQFHPEKNLYEWVRNKNISHTTNAVIASQYFAEFFVSEARKNDHH
FADAKTEDLYVIYNYPETFTGAKGSSFEQCYMFPEDVDYVKTNNAHGTYVSIIVILLALS
VLKAIE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g7662.t2 | Gene3D | G3DSA:3.40.50.880 | - | 39 | 334 | 1.3E-92 |
| 2 | g7662.t2 | PANTHER | PTHR11315 | PROTEASE FAMILY C26 GAMMA-GLUTAMYL HYDROLASE | 36 | 333 | 2.8E-98 |
| 1 | g7662.t2 | Pfam | PF07722 | Peptidase C26 | 44 | 261 | 1.6E-29 |
| 9 | g7662.t2 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 25 | - |
| 10 | g7662.t2 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 6 | - |
| 11 | g7662.t2 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 7 | 17 | - |
| 13 | g7662.t2 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 18 | 25 | - |
| 8 | g7662.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 26 | 346 | - |
| 12 | g7662.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 347 | 365 | - |
| 7 | g7662.t2 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 366 | 366 | - |
| 6 | g7662.t2 | ProSiteProfiles | PS51275 | Gamma-glutamyl hydrolase domain profile. | 35 | 334 | 109.124 |
| 3 | g7662.t2 | SUPERFAMILY | SSF52317 | Class I glutamine amidotransferase-like | 65 | 333 | 1.25E-35 |
| 4 | g7662.t2 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 25 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016787 | hydrolase activity | MF |
| GO:0008242 | omega peptidase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K01307 (gamma-glutamyl hydrolase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
