General information for g7674.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as E3 ubiquitin-protein ligase Nedd-4.
Parent gene
- The parent gene g7674 is located on chr_2: 25211918..25220249 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7674 | g7674.t2 | TTS | g7674.t2 | 25207300 | 25207300 |
| chr_2 | g7674 | g7674.t2 | isoform | g7674.t2 | 25207681 | 25210862 |
| chr_2 | g7674 | g7674.t2 | exon | g7674.t2.exon1 | 25207681 | 25208276 |
| chr_2 | g7674 | g7674.t2 | cds | g7674.t2.CDS1 | 25208174 | 25208276 |
| chr_2 | g7674 | g7674.t2 | exon | g7674.t2.exon2 | 25210222 | 25210336 |
| chr_2 | g7674 | g7674.t2 | cds | g7674.t2.CDS2 | 25210222 | 25210336 |
| chr_2 | g7674 | g7674.t2 | exon | g7674.t2.exon3 | 25210435 | 25210862 |
| chr_2 | g7674 | g7674.t2 | cds | g7674.t2.CDS3 | 25210435 | 25210831 |
| chr_2 | g7674 | g7674.t2 | TSS | g7674.t2 | NA | NA |
Sequences
>g7674.t2 Gene=g7674 Length=1139
ATGGATAAAAGAAAATGATCCGAGTGAATTAATGCTCACATTTTGTGTAGATGAAGAAAC
TTTCGGTCATACTAGTCAGAGAGATTTAAAACCAAACGGAAGCCAGATTGAAGTGACAAA
CGAAAATAAAGACGAATATATTCGCTTGGTTATTCAATGGAGATTTGAATCACGCGTCAA
AGAGCAAATGCAAGCATTCCTCGATGGATTTGGTTCAATTGTGCCACTCAATCATCTCAA
AATATTTGACGAAAACGAGCTCGAGTTGTTGATGTGTGGAATACAAAATATTGATGTGAA
AGATTGGAAGCGAAATACACTATACAAAGGCGATTATTATGCTAATCACGTCATCATTCA
ATGGTTCTGGCGAGCGGTGCTTAGCATGTCGAATGAAATGCGTGCTAGATTGCTTCAGTT
TGTAACAGGTACATCGAGAATTCCAATGAATGGAATGAAGGAACTTTATGGAAGTAATGG
ACCGCAAATGTTTACAATCGAGAAATGGGGAACGCCAGAGAATTTTCCACGTGCTCATAC
GTGCTTTAATCGATTAGACCTGCCACCATATGAGAGCTATCTTCAATTAAAGGATAAACT
TATAAGAGCAATCGAGAACTCATCAGGATTTAGCGGCGTAGATTAAATGGATTTTAAATA
ATTATTGACATTCCTTAACTCATCTTCTCTACCTTAAAACCACACAGCAGTCGCAGCAGC
AGCAAAGAAGGAATTTTTTTAAGTAAAAGTGAAATTAAAATGTGCTTTATTTATGTTAAT
CGCCAAGCCAGTTATTTTGTGGAAATTTGGAATTCATTAAGCCAAATTTTAAAAAAATTT
ATTTATGTATGATGTAAATAATGAAAAAAATACACCAACATAAATTATAAATACATTCAT
GTGCCTTTTTCATCATCAGTGAATTTATGAGAGAAAAAGAAGCGCTAAAAAATTCTCCAA
ACACGCTTCAATTGCTTACAATTTTTATCATTATTGCATTCTTACTTACTTGAAGAGACA
TCAATCAAATTAGGTCAAGAGCATGACGACGCTTCAATTAAATTCATTTGACCTTATTCA
ATGCATCGCCGATTGAGCGAATAAACTTTTTGAAATAAAAAACTTGTGAATAATAAAAA
>g7674.t2 Gene=g7674 Length=204
MLTFCVDEETFGHTSQRDLKPNGSQIEVTNENKDEYIRLVIQWRFESRVKEQMQAFLDGF
GSIVPLNHLKIFDENELELLMCGIQNIDVKDWKRNTLYKGDYYANHVIIQWFWRAVLSMS
NEMRARLLQFVTGTSRIPMNGMKELYGSNGPQMFTIEKWGTPENFPRAHTCFNRLDLPPY
ESYLQLKDKLIRAIENSSGFSGVD
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g7674.t2 | Gene3D | G3DSA:3.30.2160.10 | Hect | 1 | 48 | 0.000 |
| 7 | g7674.t2 | Gene3D | G3DSA:3.30.2410.10 | Hect | 85 | 204 | 0.000 |
| 2 | g7674.t2 | PANTHER | PTHR11254:SF420 | E3 UBIQUITIN-PROTEIN LIGASE NEDD-4 | 1 | 204 | 0.000 |
| 3 | g7674.t2 | PANTHER | PTHR11254 | HECT DOMAIN UBIQUITIN-PROTEIN LIGASE | 1 | 204 | 0.000 |
| 1 | g7674.t2 | Pfam | PF00632 | HECT-domain (ubiquitin-transferase) | 2 | 201 | 0.000 |
| 8 | g7674.t2 | ProSiteProfiles | PS50237 | HECT domain profile. | 1 | 203 | 66.333 |
| 5 | g7674.t2 | SMART | SM00119 | hect_3 | 1 | 203 | 0.000 |
| 4 | g7674.t2 | SUPERFAMILY | SSF56204 | Hect, E3 ligase catalytic domain | 2 | 197 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004842 | ubiquitin-protein transferase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
