General information for g7781.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Vesicular glutamate transporter 1.
Parent gene
- The parent gene g7781 is located on chr_2: 26084258..26085962 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7781 | g7781.t1 | isoform | g7781.t1 | 26084258 | 26085962 |
| chr_2 | g7781 | g7781.t1 | exon | g7781.t1.exon1 | 26084258 | 26084395 |
| chr_2 | g7781 | g7781.t1 | cds | g7781.t1.CDS1 | 26084258 | 26084395 |
| chr_2 | g7781 | g7781.t1 | exon | g7781.t1.exon2 | 26084454 | 26084503 |
| chr_2 | g7781 | g7781.t1 | cds | g7781.t1.CDS2 | 26084454 | 26084503 |
| chr_2 | g7781 | g7781.t1 | exon | g7781.t1.exon3 | 26084562 | 26084909 |
| chr_2 | g7781 | g7781.t1 | cds | g7781.t1.CDS3 | 26084562 | 26084909 |
| chr_2 | g7781 | g7781.t1 | exon | g7781.t1.exon4 | 26084962 | 26085345 |
| chr_2 | g7781 | g7781.t1 | cds | g7781.t1.CDS4 | 26084962 | 26085345 |
| chr_2 | g7781 | g7781.t1 | exon | g7781.t1.exon5 | 26085397 | 26085749 |
| chr_2 | g7781 | g7781.t1 | cds | g7781.t1.CDS5 | 26085397 | 26085749 |
| chr_2 | g7781 | g7781.t1 | exon | g7781.t1.exon6 | 26085802 | 26085900 |
| chr_2 | g7781 | g7781.t1 | cds | g7781.t1.CDS6 | 26085802 | 26085900 |
| chr_2 | g7781 | g7781.t1 | exon | g7781.t1.exon7 | 26085949 | 26085962 |
| chr_2 | g7781 | g7781.t1 | cds | g7781.t1.CDS7 | 26085949 | 26085962 |
| chr_2 | g7781 | g7781.t1 | TSS | g7781.t1 | 26086040 | 26086040 |
| chr_2 | g7781 | g7781.t1 | TTS | g7781.t1 | NA | NA |
Sequences
>g7781.t1 Gene=g7781 Length=1386
ATGGAAAATTCAATAATTGAAACAGCACCATTCTGGAAAAAGAAACGTTATTTAGTCGTT
CTACTTGCATTTTTTGGATTTTTTAATGTTTATGCTTTAAGAGTTAATTTAAGTGTTGCA
ATAGTATCAATGACGGAAGTAAGAGAAATTCATCATGAAAATAATACTGTGATAGAAGAG
CAATATTTTAATTGGAGTTCAAAACAGAAAGGATTAGTTTTAAGTTCATTTTTTTATGGC
TACATTACAACACAATTTATGGGCGGTTATTTGGCATCTCGATATGGTGGAAATTTAATT
TTTGGCATAGGCATAGGAGTTACTGCATTTTTTACACTTTTGACACCATTAGCCGCATCG
TGGAATATTTATGCACTTGTTGCTGTAAGAGTGATTGAAGGAATTTTTGAAGGTATGACT
TTTCCATGTTGTTATCACATTTGGAGTAAATGGGCACCTCCTTTAGAGAGATCAAGAATG
GCTAACTTTGCAATGGCTGGAAATTATGTTGGAACTGTCGTCGGAATGCCTTTATCAGGC
ATTTTTGCAACTTATATCGGTTGGCAGAGTGTTTTCTACATTTTTGGTACTATTGGAATT
GTATGGCTTATTATGTGGATTTTAATAGTTAAAGCAAGTCCAGAAGCTGATTCTCGTATA
TCAAAAATTGAAAGAGACTACATTATTGCTAGTATAGGACGGAATGCAAATGAAAAACCA
CCAAATTTTAATGAAATTCCATGGAAGGATATATTCATGTCAAAAGCTGTTTGGGCAATC
ATTGTTGCATTCTTCTGTGAGAGTTGGGGATTTTTCACGATGTTTACTGAACTTCCTTCT
TTTTTTAAAGATCTTTTGGACTTTGATATTTCCAAATCAGCAATTTTATCAGCTCTTCCT
TATTTAGCACTTACAATTCTTCTCTTTTTCTCGGGTTTCATTGCTGATTGGTTTCAAGTG
AAATCAATTTTAACTACAACTCAAGTTCGTCGCTATTTCACTTGCATTTCATTTTTAAGT
CAAACAATTTTCATGATTCTTGCTGCTTTTATTCTTCATCGAGTTTATTCAGTTATATTT
TTGACAATTGGTATTGGGTTGGGGGCATTTTCACTATCTGGATTTGCAATTAATCATTTA
GATATTGCACCACAATATGCGAGTATATTGATGGGAATTGGAAATACTTTTGGAACAATT
CCTGGCATGGTTTCACCTTTATTAACTGGATATATTGTTACAAAATCTACGGAAAGTGAA
TGGAGGATTGTTTTTTATATTACAGCAGCACTTTATATTTCTGGATGTATATTTTATTGG
TTCTTCTCTTCTGGGAAAGTTCAAGCATGGGCTGAGAAGAAAAATGAAATAAAGAATAAA
ATTTAA
>g7781.t1 Gene=g7781 Length=461
MENSIIETAPFWKKKRYLVVLLAFFGFFNVYALRVNLSVAIVSMTEVREIHHENNTVIEE
QYFNWSSKQKGLVLSSFFYGYITTQFMGGYLASRYGGNLIFGIGIGVTAFFTLLTPLAAS
WNIYALVAVRVIEGIFEGMTFPCCYHIWSKWAPPLERSRMANFAMAGNYVGTVVGMPLSG
IFATYIGWQSVFYIFGTIGIVWLIMWILIVKASPEADSRISKIERDYIIASIGRNANEKP
PNFNEIPWKDIFMSKAVWAIIVAFFCESWGFFTMFTELPSFFKDLLDFDISKSAILSALP
YLALTILLFFSGFIADWFQVKSILTTTQVRRYFTCISFLSQTIFMILAAFILHRVYSVIF
LTIGIGLGAFSLSGFAINHLDIAPQYASILMGIGNTFGTIPGMVSPLLTGYIVTKSTESE
WRIVFYITAALYISGCIFYWFFSSGKVQAWAEKKNEIKNKI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 34 | g7781.t1 | CDD | cd17318 | MFS_SLC17 | 21 | 442 | 1.76012E-163 |
| 6 | g7781.t1 | Gene3D | G3DSA:1.20.1250.20 | MFS general substrate transporter like domains | 10 | 219 | 9.3E-42 |
| 5 | g7781.t1 | Gene3D | G3DSA:1.20.1250.20 | MFS general substrate transporter like domains | 243 | 454 | 1.8E-32 |
| 2 | g7781.t1 | PANTHER | PTHR11662 | SOLUTE CARRIER FAMILY 17 | 12 | 456 | 5.5E-178 |
| 3 | g7781.t1 | PANTHER | PTHR11662:SF437 | GH23975P | 12 | 456 | 5.5E-178 |
| 1 | g7781.t1 | Pfam | PF07690 | Major Facilitator Superfamily | 24 | 406 | 7.8E-58 |
| 19 | g7781.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 32 | - |
| 20 | g7781.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 17 | - |
| 21 | g7781.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 18 | 28 | - |
| 33 | g7781.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 29 | 32 | - |
| 14 | g7781.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 33 | 71 | - |
| 31 | g7781.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 72 | 92 | - |
| 10 | g7781.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 93 | 98 | - |
| 24 | g7781.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 99 | 117 | - |
| 17 | g7781.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 118 | 122 | - |
| 22 | g7781.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 123 | 148 | - |
| 12 | g7781.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 149 | 159 | - |
| 25 | g7781.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 160 | 185 | - |
| 16 | g7781.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 186 | 190 | - |
| 23 | g7781.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 191 | 210 | - |
| 7 | g7781.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 211 | 255 | - |
| 30 | g7781.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 256 | 275 | - |
| 15 | g7781.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 276 | 294 | - |
| 27 | g7781.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 295 | 320 | - |
| 11 | g7781.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 321 | 331 | - |
| 32 | g7781.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 332 | 352 | - |
| 13 | g7781.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 353 | 357 | - |
| 26 | g7781.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 358 | 377 | - |
| 9 | g7781.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 378 | 388 | - |
| 29 | g7781.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 389 | 411 | - |
| 18 | g7781.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 412 | 422 | - |
| 28 | g7781.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 423 | 442 | - |
| 8 | g7781.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 443 | 461 | - |
| 47 | g7781.t1 | ProSiteProfiles | PS50850 | Major facilitator superfamily (MFS) profile. | 18 | 447 | 28.69 |
| 4 | g7781.t1 | SUPERFAMILY | SSF103473 | MFS general substrate transporter | 9 | 451 | 1.83E-78 |
| 39 | g7781.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 20 | 42 | - |
| 36 | g7781.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 73 | 92 | - |
| 45 | g7781.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 99 | 121 | - |
| 42 | g7781.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 126 | 148 | - |
| 38 | g7781.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 161 | 183 | - |
| 35 | g7781.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 188 | 210 | - |
| 46 | g7781.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 256 | 278 | - |
| 37 | g7781.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 293 | 315 | - |
| 40 | g7781.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 335 | 352 | - |
| 44 | g7781.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 356 | 378 | - |
| 41 | g7781.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 391 | 413 | - |
| 43 | g7781.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 423 | 445 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0055085 | transmembrane transport | BP |
| GO:0022857 | transmembrane transporter activity | MF |
KEGG
Orthology
- This transcript has been annotated with K12301 (MFS transporter, ACS family, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 5)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed