Gene loci information

Transcript annotation

  • This transcript has been annotated as Cytochrome P450 9e2.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g7913 g7913.t1 TTS g7913.t1 27024850 27024850
chr_2 g7913 g7913.t1 isoform g7913.t1 27025516 27027087
chr_2 g7913 g7913.t1 exon g7913.t1.exon1 27025516 27027087
chr_2 g7913 g7913.t1 cds g7913.t1.CDS1 27025516 27027087
chr_2 g7913 g7913.t1 TSS g7913.t1 27027138 27027138

Sequences

>g7913.t1 Gene=g7913 Length=1572
ATGATTTTTTGGATAATTATTTTTCTCCTCTTTCTAATTTACAAGTGGCTTACAAGCGAT
CATGACTATTTTAAAAAAATTAATGTGCCATTTGATAGGCCATTGCCACTTGTTGGCAAT
TTATTAGGTATTGTGCTTCAAAAAGAATCAATTGTTGAAATTACAACAAGAAACTACTAC
AAATATAAAGATTACAAAGTTTATGGATTTTTCAATTTCCTTCAACCTTCATTCATGATT
ACTGATCCTGAATTAATAAAGAAAATTACAATAAAAGACTTTGATCATTTTCTAAACCAT
ATAGAGGCATTCGAAATCGATCGTCTTTTCTCTCGTACAGTGTTCGCTATGCGAGATAAG
AAATGGAGAGATATGAGAACGACATTGAGTCCAATATTCACAAGTTCAAAAATGAAAATT
ATGTTTGGTCTTTTGGGAGAACAATCAAATGATTTTGTGAAATTTATGCATGAGCAAGTT
GAAAGTGGTAAAAATTTGAATGTAGAAGTAGCTGAAATTTTTAGTCGTTACACAGCAGAT
GCAATTTCTACAGCAGCACTCGGTTTCAAAGGAGAATCTGTACGGAATGAAAAATGTGAA
GCTTACACAATTTTAAAATTGATGCAAAATAATTTTAATGGGTCATTAGGTGCATTAAAA
TTTTTGCTTGCCTTCACACTTCCAAAAGTTTATAAATTCTTTGATATTCAATTAGTTGGA
AAGGAAGTTAATGACTTTTTCAAGAGAATTGTTATTGATGAAATAAATTTAAGAGAAAAG
AAGAATTTCTATCGACCTGATATGATTCAATTACTCCTGCAAGCAAGAAAAGGACAATTG
AAAAATGAAGAAATAAATGAAAATGATTTATCAAATTTTTCTGCACATACAGAATTTGAT
GTTAGTTTAAAAAATGAAAAAATGTCACATTTTGAAGATCTTGATTGGATTGCGCAAGGG
TTTGTGATGTTTGGAGCTGGTTTCGATACCTCTACAGATTTACTTCAAATAATTTGCTAC
GAGCTTTCATTTAATAAAAACATTCAAAATGATTTAATTGCAGAAATTGATAAAATTGAT
GCGGTGGAGACAATAAGTTATGAGACATTGCACAAGATGAAATTTTTGGACATGGTTGTT
TCAGAAACATTAAGAAAATGGCCTCCTGCAGGTCAACTTGATAGATGCTGTAATAAGGAA
TATAAATTAGATTTGGAAAATGGAAAATCAGTGATAATTGAAAAGGGTAAAAATGTTTTC
TTACCAGTGTATCAAATTCATCATGATCCTGATTATTATACAGAACCAGAAAAGTTTAAT
CCATATCGATTTAGTGATGAGAATAAAGAGAATTTAGTGCCAGGGACATTTTTGCCTTTT
GGCATCGGACCACGCGCTTGTTTAGCTTCTCGATTTGCTTTAATGTCGGTTAAATTGATT
TTGGTAAAAATTTTAAATGACTTTAAAATTGAGCCTTGTGAAAAGACACCAGCAAAATTG
ACATATCAAGCAAACATCAACTTCAAATTCAACGAGAAAATTTTTCTAAATTTCATCCCA
AGAAGCAAATAA

>g7913.t1 Gene=g7913 Length=523
MIFWIIIFLLFLIYKWLTSDHDYFKKINVPFDRPLPLVGNLLGIVLQKESIVEITTRNYY
KYKDYKVYGFFNFLQPSFMITDPELIKKITIKDFDHFLNHIEAFEIDRLFSRTVFAMRDK
KWRDMRTTLSPIFTSSKMKIMFGLLGEQSNDFVKFMHEQVESGKNLNVEVAEIFSRYTAD
AISTAALGFKGESVRNEKCEAYTILKLMQNNFNGSLGALKFLLAFTLPKVYKFFDIQLVG
KEVNDFFKRIVIDEINLREKKNFYRPDMIQLLLQARKGQLKNEEINENDLSNFSAHTEFD
VSLKNEKMSHFEDLDWIAQGFVMFGAGFDTSTDLLQIICYELSFNKNIQNDLIAEIDKID
AVETISYETLHKMKFLDMVVSETLRKWPPAGQLDRCCNKEYKLDLENGKSVIIEKGKNVF
LPVYQIHHDPDYYTEPEKFNPYRFSDENKENLVPGTFLPFGIGPRACLASRFALMSVKLI
LVKILNDFKIEPCEKTPAKLTYQANINFKFNEKIFLNFIPRSK

Protein features from InterProScan

Transcript Database ID Name Start End E.value
14 g7913.t1 Gene3D G3DSA:1.10.630.10 Cytochrome p450 12 523 5.1E-117
2 g7913.t1 PANTHER PTHR24292 CYTOCHROME P450 12 509 2.7E-149
3 g7913.t1 PANTHER PTHR24292:SF90 CYTOCHROME P450 317A1-RELATED 12 509 2.7E-149
6 g7913.t1 PRINTS PR00385 P450 superfamily signature 326 343 2.0E-7
8 g7913.t1 PRINTS PR00465 E-class P450 group IV signature 373 389 2.4E-14
4 g7913.t1 PRINTS PR00385 P450 superfamily signature 378 389 2.0E-7
9 g7913.t1 PRINTS PR00465 E-class P450 group IV signature 427 445 2.4E-14
11 g7913.t1 PRINTS PR00465 E-class P450 group IV signature 451 467 2.4E-14
5 g7913.t1 PRINTS PR00385 P450 superfamily signature 458 467 2.0E-7
7 g7913.t1 PRINTS PR00385 P450 superfamily signature 467 478 2.0E-7
10 g7913.t1 PRINTS PR00465 E-class P450 group IV signature 467 485 2.4E-14
1 g7913.t1 Pfam PF00067 Cytochrome P450 31 504 6.0E-74
16 g7913.t1 Phobius SIGNAL_PEPTIDE Signal peptide region 1 20 -
17 g7913.t1 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 1 -
18 g7913.t1 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 2 13 -
19 g7913.t1 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 14 20 -
15 g7913.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 21 523 -
20 g7913.t1 ProSitePatterns PS00086 Cytochrome P450 cysteine heme-iron ligand signature. 460 469 -
12 g7913.t1 SUPERFAMILY SSF48264 Cytochrome P450 30 521 2.88E-94
13 g7913.t1 SignalP_EUK SignalP-noTM SignalP-noTM 1 19 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0004497 monooxygenase activity MF
GO:0055114 NA NA

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed