Gene loci information

Transcript annotation

  • This transcript has been annotated as Cytochrome P450 9e2.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g7913 g7913.t2 TTS g7913.t2 27024850 27024850
chr_2 g7913 g7913.t2 isoform g7913.t2 27024996 27027125
chr_2 g7913 g7913.t2 exon g7913.t2.exon1 27024996 27027042
chr_2 g7913 g7913.t2 cds g7913.t2.CDS1 27025516 27026853
chr_2 g7913 g7913.t2 exon g7913.t2.exon2 27027108 27027125
chr_2 g7913 g7913.t2 TSS g7913.t2 27027138 27027138

Sequences

>g7913.t2 Gene=g7913 Length=2065
GCTCGCTTGCGATAAAACTGGCTTACAAGCGATCATGACTATTTTAAAAAAATTAATGTG
CCATTTGATAGGCCATTGCCACTTGTTGGCAATTTATTAGGTATTGTGCTTCAAAAAGAA
TCAATTGTTGAAATTACAACAAGAAACTACTACAAATATAAAGATTACAAAGTTTATGGA
TTTTTCAATTTCCTTCAACCTTCATTCATGATTACTGATCCTGAATTAATAAAGAAAATT
ACAATAAAAGACTTTGATCATTTTCTAAACCATATAGAGGCATTCGAAATCGATCGTCTT
TTCTCTCGTACAGTGTTCGCTATGCGAGATAAGAAATGGAGAGATATGAGAACGACATTG
AGTCCAATATTCACAAGTTCAAAAATGAAAATTATGTTTGGTCTTTTGGGAGAACAATCA
AATGATTTTGTGAAATTTATGCATGAGCAAGTTGAAAGTGGTAAAAATTTGAATGTAGAA
GTAGCTGAAATTTTTAGTCGTTACACAGCAGATGCAATTTCTACAGCAGCACTCGGTTTC
AAAGGAGAATCTGTACGGAATGAAAAATGTGAAGCTTACACAATTTTAAAATTGATGCAA
AATAATTTTAATGGGTCATTAGGTGCATTAAAATTTTTGCTTGCCTTCACACTTCCAAAA
GTTTATAAATTCTTTGATATTCAATTAGTTGGAAAGGAAGTTAATGACTTTTTCAAGAGA
ATTGTTATTGATGAAATAAATTTAAGAGAAAAGAAGAATTTCTATCGACCTGATATGATT
CAATTACTCCTGCAAGCAAGAAAAGGACAATTGAAAAATGAAGAAATAAATGAAAATGAT
TTATCAAATTTTTCTGCACATACAGAATTTGATGTTAGTTTAAAAAATGAAAAAATGTCA
CATTTTGAAGATCTTGATTGGATTGCGCAAGGGTTTGTGATGTTTGGAGCTGGTTTCGAT
ACCTCTACAGATTTACTTCAAATAATTTGCTACGAGCTTTCATTTAATAAAAACATTCAA
AATGATTTAATTGCAGAAATTGATAAAATTGATGCGGTGGAGACAATAAGTTATGAGACA
TTGCACAAGATGAAATTTTTGGACATGGTTGTTTCAGAAACATTAAGAAAATGGCCTCCT
GCAGGTCAACTTGATAGATGCTGTAATAAGGAATATAAATTAGATTTGGAAAATGGAAAA
TCAGTGATAATTGAAAAGGGTAAAAATGTTTTCTTACCAGTGTATCAAATTCATCATGAT
CCTGATTATTATACAGAACCAGAAAAGTTTAATCCATATCGATTTAGTGATGAGAATAAA
GAGAATTTAGTGCCAGGGACATTTTTGCCTTTTGGCATCGGACCACGCGCTTGTTTAGCT
TCTCGATTTGCTTTAATGTCGGTTAAATTGATTTTGGTAAAAATTTTAAATGACTTTAAA
ATTGAGCCTTGTGAAAAGACACCAGCAAAATTGACATATCAAGCAAACATCAACTTCAAA
TTCAACGAGAAAATTTTTCTAAATTTCATCCCAAGAAGCAAATAAGGATGAGAGACAACT
AAAAATTAATTATATACATCCGAGAATCATTTTTTAAAAAAGGAATCTTTAACGTCACTC
AATGGATCATTTTCAACTTTAAATATTTGTTTTATCTTTTTCCACACCCAATTCAATACT
TATCTTACCTTCATTCTACTGGCTAAAGTGTACCCATTTTCACATTAATAACACATCTCC
TCTCTCTACCTTGTTACTTATATGTCTCCTTTTTCATGTCCATCCGACACACAAATCCAT
TGATAATTATCTAGTTTTTTGTAAAATCATCCTCTCATCTTTCTCCGGATGCGAAATGCA
ATTACCTGTTTTCTTGCATTCCACAACAGAACACACTTTACTAAAATGTGTAATCACAGT
CACTAAAATTGTTTTATTGTTTTTAAAAGAAAAGTTAAGATAGATAGAAGAAGAGCATTT
TTAGTGTTTGCAAAGAAGTTCTAAAAAAATTGACATAATAAGTGAAATAAATTTTTTTTG
TTTATAACTTCCGATGCATAAAATT

>g7913.t2 Gene=g7913 Length=445
MITDPELIKKITIKDFDHFLNHIEAFEIDRLFSRTVFAMRDKKWRDMRTTLSPIFTSSKM
KIMFGLLGEQSNDFVKFMHEQVESGKNLNVEVAEIFSRYTADAISTAALGFKGESVRNEK
CEAYTILKLMQNNFNGSLGALKFLLAFTLPKVYKFFDIQLVGKEVNDFFKRIVIDEINLR
EKKNFYRPDMIQLLLQARKGQLKNEEINENDLSNFSAHTEFDVSLKNEKMSHFEDLDWIA
QGFVMFGAGFDTSTDLLQIICYELSFNKNIQNDLIAEIDKIDAVETISYETLHKMKFLDM
VVSETLRKWPPAGQLDRCCNKEYKLDLENGKSVIIEKGKNVFLPVYQIHHDPDYYTEPEK
FNPYRFSDENKENLVPGTFLPFGIGPRACLASRFALMSVKLILVKILNDFKIEPCEKTPA
KLTYQANINFKFNEKIFLNFIPRSK

Protein features from InterProScan

Transcript Database ID Name Start End E.value
14 g7913.t2 Gene3D G3DSA:1.10.630.10 Cytochrome p450 1 445 5.6E-103
2 g7913.t2 PANTHER PTHR24292 CYTOCHROME P450 1 431 5.3E-138
3 g7913.t2 PANTHER PTHR24292:SF90 CYTOCHROME P450 317A1-RELATED 1 431 5.3E-138
5 g7913.t2 PRINTS PR00385 P450 superfamily signature 248 265 1.1E-7
10 g7913.t2 PRINTS PR00465 E-class P450 group IV signature 295 311 1.4E-14
7 g7913.t2 PRINTS PR00385 P450 superfamily signature 300 311 1.1E-7
9 g7913.t2 PRINTS PR00465 E-class P450 group IV signature 349 367 1.4E-14
8 g7913.t2 PRINTS PR00465 E-class P450 group IV signature 373 389 1.4E-14
4 g7913.t2 PRINTS PR00385 P450 superfamily signature 380 389 1.1E-7
6 g7913.t2 PRINTS PR00385 P450 superfamily signature 389 400 1.1E-7
11 g7913.t2 PRINTS PR00465 E-class P450 group IV signature 389 407 1.4E-14
1 g7913.t2 Pfam PF00067 Cytochrome P450 1 426 5.1E-68
13 g7913.t2 ProSitePatterns PS00086 Cytochrome P450 cysteine heme-iron ligand signature. 382 391 -
12 g7913.t2 SUPERFAMILY SSF48264 Cytochrome P450 1 443 1.44E-86

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0004497 monooxygenase activity MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed