General information for g7919.t7
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Spermine synthase.
Parent gene
- The parent gene g7919 is located on chr_2: 27065139..27067191 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7919 | g7919.t7 | TTS | g7919.t7 | 27063778 | 27063778 |
| chr_2 | g7919 | g7919.t7 | isoform | g7919.t7 | 27064007 | 27066755 |
| chr_2 | g7919 | g7919.t7 | exon | g7919.t7.exon1 | 27064007 | 27064175 |
| chr_2 | g7919 | g7919.t7 | cds | g7919.t7.CDS1 | 27064007 | 27064175 |
| chr_2 | g7919 | g7919.t7 | exon | g7919.t7.exon2 | 27064240 | 27064783 |
| chr_2 | g7919 | g7919.t7 | cds | g7919.t7.CDS2 | 27064240 | 27064783 |
| chr_2 | g7919 | g7919.t7 | exon | g7919.t7.exon3 | 27066134 | 27066755 |
| chr_2 | g7919 | g7919.t7 | cds | g7919.t7.CDS3 | 27066134 | 27066212 |
| chr_2 | g7919 | g7919.t7 | TSS | g7919.t7 | 27066841 | 27066841 |
Sequences
>g7919.t7 Gene=g7919 Length=1335
ATGGCTGCTAACTCAGTTCTACTAGATTTTTCTATAGAACCATCGCGGATTGGTGATGAA
TTATCGCGAAAGGACATCGTGAAAGTGTTCAAAGAAAATTTGGAAAAATATTTTGGCAAT
TTGAAAATTGTTTATGATATGCTTATTGATGATGGTTATATGTGCATTCTAAGTGATAAT
GCTGGCACTATCTTTACTATAAGATTCTTTGGCGAAGGTCTAATTACCGTTAACATCGAG
TATTTTAAGAAGGAAAATGACTCCCAAAGAATTTCATTTGAGGTAAAAAAATCATTTCTT
TTGCTCACGAGAATTTCATTTTCCACATTGGTCCAATCTGGTTTAGAATTTACTCAATTT
TCAAAAAAAAAAAAATGAGAGAAAAATGGAAATTTTACATGTGCGTGTCATTTCACCAAC
TGGCCATCTCATAATCATTTAATATTGTTGTTGTAATTTTTCATGTCAATGATAACCAAA
TGCTATGGTTATATGAATTTGTAATAATTATAGACATTGAGATTCCTCGAAATTAACTTG
AGAATGAAGTTAGAAGCTAAGAGATCAAAGTTATTACCAGCAATTAAGCGAGGAGGCATT
GATGTATATCTTACTAGTTCAGATGAGCGGATCATTGAGTATGATATTGACAAGGTATTA
TTTGATAAACGTTCTGAATTCCAAAAGATTCAGATCGTGCATTCGAAAACTCTTGGCAAT
ATGTTGATCCTTGATGAATTGCAAAACATTGCTGAAGCTGATTTGATTTACACTCAAACA
TTGATGCAACATGGCAAAGAAAATTATGAAGGAAAAGAAATCTGCATTCTGGGTGGTGGT
GATGGTGCTTTGCTCTATGAGTTATTAAAAGAAAATCCTAAACATGTTGTTATGCTCGAA
ATTGATGATCTCGTCATGGAAGCTTGTAACAAGTACATGAATTCAATTTGTGGCGATGTT
CTTGAAACTCGCAAAGGCGATAATTATGAAATTATCGTTGGAGATTGCATGGTTTGGATT
GATAAATATATCAAAGATGGAAAAAAGTTTGACTATGTTTTCGGTGATCTCACTGATATT
CCAATCTCTGATACGCCAACTGGAGAAATTTGGGATTTTATTCGCACCATTTTGGAGAAA
TCTTTTGCTGTCTTGAAACCTGATGGTAAATTCATGACTCATGGCAATGGCGCATCATGC
CCAGAATCTCTTGAGATGTATGAAGAGCAGTTGAAAAAACTTCGACCATGCGTTAAATTT
TCACGCGACACCGCATTTGTTCCATCATTCATGGAGGATTGGATCTTTTATCAAGTCACA
TTTGCTAATCAGTGA
>g7919.t7 Gene=g7919 Length=263
MKLEAKRSKLLPAIKRGGIDVYLTSSDERIIEYDIDKVLFDKRSEFQKIQIVHSKTLGNM
LILDELQNIAEADLIYTQTLMQHGKENYEGKEICILGGGDGALLYELLKENPKHVVMLEI
DDLVMEACNKYMNSICGDVLETRKGDNYEIIVGDCMVWIDKYIKDGKKFDYVFGDLTDIP
ISDTPTGEIWDFIRTILEKSFAVLKPDGKFMTHGNGASCPESLEMYEEQLKKLRPCVKFS
RDTAFVPSFMEDWIFYQVTFANQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g7919.t7 | CDD | cd02440 | AdoMet_MTases | 96 | 213 | 0.00000 |
| 7 | g7919.t7 | Gene3D | G3DSA:2.30.140.10 | - | 17 | 71 | 0.00000 |
| 8 | g7919.t7 | Gene3D | G3DSA:3.40.50.150 | Vaccinia Virus protein VP39 | 72 | 261 | 0.00000 |
| 5 | g7919.t7 | Hamap | MF_00198 | Polyamine aminopropyltransferase [speE]. | 21 | 262 | 14.75276 |
| 3 | g7919.t7 | PANTHER | PTHR46315:SF1 | SPERMINE SYNTHASE | 4 | 262 | 0.00000 |
| 4 | g7919.t7 | PANTHER | PTHR46315 | SPERMINE SYNTHASE | 4 | 262 | 0.00000 |
| 1 | g7919.t7 | Pfam | PF17284 | Spermidine synthase tetramerisation domain | 27 | 65 | 0.00000 |
| 2 | g7919.t7 | Pfam | PF01564 | Spermine/spermidine synthase domain | 75 | 258 | 0.00000 |
| 9 | g7919.t7 | ProSiteProfiles | PS51006 | Polyamine biosynthesis (PABS) domain profile. | 20 | 261 | 36.59600 |
| 6 | g7919.t7 | SUPERFAMILY | SSF53335 | S-adenosyl-L-methionine-dependent methyltransferases | 17 | 258 | 0.00000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016768 | spermine synthase activity | MF |
| GO:0006597 | spermine biosynthetic process | BP |
| GO:0003824 | catalytic activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
