Gene loci information

Transcript annotation

  • This transcript has been annotated as Long-chain-fatty-acid–CoA ligase 3.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g7969 g7969.t2 TTS g7969.t2 27328362 27328362
chr_2 g7969 g7969.t2 isoform g7969.t2 27328555 27330501
chr_2 g7969 g7969.t2 exon g7969.t2.exon1 27328555 27328985
chr_2 g7969 g7969.t2 cds g7969.t2.CDS1 27328555 27328985
chr_2 g7969 g7969.t2 exon g7969.t2.exon2 27329043 27329351
chr_2 g7969 g7969.t2 cds g7969.t2.CDS2 27329043 27329351
chr_2 g7969 g7969.t2 exon g7969.t2.exon3 27329420 27329485
chr_2 g7969 g7969.t2 cds g7969.t2.CDS3 27329420 27329485
chr_2 g7969 g7969.t2 exon g7969.t2.exon4 27329551 27330087
chr_2 g7969 g7969.t2 cds g7969.t2.CDS4 27329551 27330087
chr_2 g7969 g7969.t2 exon g7969.t2.exon5 27330164 27330367
chr_2 g7969 g7969.t2 cds g7969.t2.CDS5 27330164 27330320
chr_2 g7969 g7969.t2 exon g7969.t2.exon6 27330439 27330501
chr_2 g7969 g7969.t2 TSS g7969.t2 27331165 27331165

Sequences

>g7969.t2 Gene=g7969 Length=1610
TTAAACATAGTTGCACCATTGTAACAATTTATGCCACACTTGGTGAAGAAGGAATCATTC
ATGGTGTTAATGAGACAGAAGTATCAGTGATAATCACATCACATGAATTGATGCCAAAAC
TCCGAAGCATTCTCGATAAAATTCCAAAGGTTACAACTATCATCTTCTTTGAAGATCAAT
TACAGAAAACCGATACAAAGGGATTCGAGCGAATCAAAACAATTTCATATTCCAAAGTCA
TTGAGGCGGGCATTAAAAATAAAGTTGAACCGGTTGCTCCGTCAACAAATGATACCGCCA
TAATTATGTATACCTCTGGAAGTACTGGAACTCCAAAAGGTGTGCTTATTTCACATGGCA
ATTGTATTGCAACTTTAAAATGCTTCTGTGATGTTGTCGAGATTCATCCCGATGATATCC
TTCTTGGCTTTTTGCCACTGGCTCATGTCTTCGAATTGCTTGCCGAGAGTGTATGTTTGC
TTACTGGCGTTCCAATTGGCTACTCTTCGCCTAATACCCTCATTGATAGTAGCACAAAAA
TCATGAAGGGCAGTAAGGGCGACGCATCGGTCCTTAGACCCACTTGCATCACGGCCGTTC
CACTAATTCTAGATAGAATTTGTAAGGGTATCACAGATAAAATTAACAAGGGATCTCCAC
TACAAAAGGCTATTTTTAAGTATTGCTACGACTATAAGGTTTTATGGTGTAGACGAGGAT
TTAACACTCCACTGTTGGACTTCATTATTTTTAAGAAAATCACAAAGCTTATGGGCGGTC
GCTTAAGATTGATGATTTCAGGAGGCGCTCCACTTAGTGCAGAAACTCATGAACAAATTC
GTTTATGTCTCTGTGTCGATGTCTGTCAAGGCTATGGGTTGACAGAAACAACAACAGCTT
CATGTGTAATGGATAAACATGATATGACTTATGGACGTGTTGGTGCACCATCATCATTGG
TTGATTTGCGCTTAATTAATTGGGAAGAAGGAAATTATCGTGTGACAAATAAGCCCTATC
CTCAGGGAGAAATTGTTATTGGCTCAAAGAGTGTTGCAATGGGTTATTATAAAATGCCAG
AATTATCAAAAGAAAGCTTCTTTGAAGAAGATGGCAAAAGATGGTTTAGAACTGGTGACA
TTGGTGAAATTTATCCTGATGGTGTTTTAAAAATCATTGATCGTAAAAAAGATTTAGTGA
AGCTTCAAGCCGGTGAATATGTGTCGCTCGGACGTGTCGAAGCTGAAATGAAGACATGTC
CATTAGTTGAAAATATTTGTGTTTATGGTGATCCTTCAAAACATTATTGTGTAGCTTTGG
TTGTACCAGCTGAAAAAATGCTAATGGAATTAGCTGAGAATTTAAATATTCATAATAAAA
ATTTTGAAGAGCTCTGTGCATCGCCAATAATTGAAAAAGCAGTGGCAAGGGAATTAATTG
AGCATGCTAAAAAATGCAAATTAGAAAAGTTTGAAATTCCTGCAGCCATAACATTATGCA
AAGACACATGGACGACTGAAACTGGAATGGTCACAGCAGCTTTTAAGATTAAACGAAAGG
TCATTCAGGAACGTTATAAAGAGGAAATCAAGAGGATGTATGCTTCATAA

>g7969.t2 Gene=g7969 Length=499
MPKLRSILDKIPKVTTIIFFEDQLQKTDTKGFERIKTISYSKVIEAGIKNKVEPVAPSTN
DTAIIMYTSGSTGTPKGVLISHGNCIATLKCFCDVVEIHPDDILLGFLPLAHVFELLAES
VCLLTGVPIGYSSPNTLIDSSTKIMKGSKGDASVLRPTCITAVPLILDRICKGITDKINK
GSPLQKAIFKYCYDYKVLWCRRGFNTPLLDFIIFKKITKLMGGRLRLMISGGAPLSAETH
EQIRLCLCVDVCQGYGLTETTTASCVMDKHDMTYGRVGAPSSLVDLRLINWEEGNYRVTN
KPYPQGEIVIGSKSVAMGYYKMPELSKESFFEEDGKRWFRTGDIGEIYPDGVLKIIDRKK
DLVKLQAGEYVSLGRVEAEMKTCPLVENICVYGDPSKHYCVALVVPAEKMLMELAENLNI
HNKNFEELCASPIIEKAVARELIEHAKKCKLEKFEIPAAITLCKDTWTTETGMVTAAFKI
KRKVIQERYKEEIKRMYAS

Protein features from InterProScan

Transcript Database ID Name Start End E.value
7 g7969.t2 Gene3D G3DSA:3.40.50.12780 - 3 192 2.6E-25
8 g7969.t2 Gene3D G3DSA:3.40.50.12780 - 206 361 1.1E-32
9 g7969.t2 Gene3D G3DSA:3.30.300.30 - 362 497 8.4E-8
3 g7969.t2 PANTHER PTHR43272:SF5 ACYL-COA SYNTHETASE LONG-CHAIN, ISOFORM J 3 497 1.4E-176
4 g7969.t2 PANTHER PTHR43272 LONG-CHAIN-FATTY-ACID–COA LIGASE 3 497 1.4E-176
2 g7969.t2 Pfam PF00501 AMP-binding enzyme 3 366 1.3E-72
1 g7969.t2 Pfam PF13193 AMP-binding enzyme C-terminal domain 376 469 8.5E-7
6 g7969.t2 ProSitePatterns PS00455 Putative AMP-binding domain signature. 65 76 -
5 g7969.t2 SUPERFAMILY SSF56801 Acetyl-CoA synthetase-like 2 497 2.49E-89

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

There are no GO annotations for this transcript.

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values