General information for g8055.t6
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Vacuolar protein sorting-associated protein 35.
Parent gene
- The parent gene g8055 is located on chr_2: 28279164..28281357 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8055 | g8055.t6 | TSS | g8055.t6 | 28278423 | 28278423 |
| chr_2 | g8055 | g8055.t6 | isoform | g8055.t6 | 28278425 | 28279164 |
| chr_2 | g8055 | g8055.t6 | exon | g8055.t6.exon1 | 28278425 | 28279164 |
| chr_2 | g8055 | g8055.t6 | cds | g8055.t6.CDS1 | 28278505 | 28279164 |
| chr_2 | g8055 | g8055.t6 | TTS | g8055.t6 | 28279038 | 28279038 |
Sequences
>g8055.t6 Gene=g8055 Length=740
CTCAATCACCGACTCAGCTGGATGAGCAAGATAAGCTCTTAAGCGAGGCACTAGGTGTCG
TTCGCAATAATGCAATTCTTATGAAGCGAAATCTTGATAAAAATCGCCTAATGGATGGCC
TTAAGTGTGCTAGTACAATGTTGGGTGAATTACGAACAAGTTTGTTATCACCAAAAAGCT
ATTATGAGTTGTACATGGCGGTGACTGATGAATTGCGTTTTCTTGAAGTTTATTTACTGG
ATGAATTTCAAAAAGGTCATAAAATCACTGATTTGTATGAATTAGTACAATATGCGGGCA
ATATTGTTCCACGCCTCTATTTGCTGATAACAGTTGGATTAGTTTACATCAAATCAAATC
CTATGACTAAAAAGAGCATTTTAAAAGATTTAGTTGAGATGTGTCGTGGAGTTCAAAATC
CATTGAGAGGATTATTTCTACGTAATTATCTTCTCACCCTACGCAACATTTTACCCGATA
CTCCTGTTGGAAAAGATGACGAAACTGGCAACGTGCATGATTCGATTGACTTTATTTTAA
CAAATTTCGCTGAAATGAACAAATTGTGGGTGAGAATGCAACATCAAGGACATTCAAGTG
AAAAATCAAGACGTGAAAAAGAAAGAGAAGAATTGAAAATTCTTGTTGGTACGAATTTAG
TGCGTTTATCGCAACTGGACTCTGTCAATCTTGAAATTTATCAAAAAATCATTCTACCGA
GTATTCTTGAACAAGTTGTA
>g8055.t6 Gene=g8055 Length=220
MKRNLDKNRLMDGLKCASTMLGELRTSLLSPKSYYELYMAVTDELRFLEVYLLDEFQKGH
KITDLYELVQYAGNIVPRLYLLITVGLVYIKSNPMTKKSILKDLVEMCRGVQNPLRGLFL
RNYLLTLRNILPDTPVGKDDETGNVHDSIDFILTNFAEMNKLWVRMQHQGHSSEKSRREK
EREELKILVGTNLVRLSQLDSVNLEIYQKIILPSILEQVV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 2 | g8055.t6 | PANTHER | PTHR11099 | VACUOLAR SORTING PROTEIN 35 | 1 | 220 | 6.8E-117 |
| 3 | g8055.t6 | PANTHER | PTHR11099:SF0 | VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 35 | 1 | 220 | 6.8E-117 |
| 1 | g8055.t6 | Pfam | PF03635 | Vacuolar protein sorting-associated protein 35 | 1 | 220 | 1.4E-114 |
| 5 | g8055.t6 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 70 | - |
| 6 | g8055.t6 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 71 | 90 | - |
| 4 | g8055.t6 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 91 | 220 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0042147 | retrograde transport, endosome to Golgi | BP |
| GO:0030906 | retromer, cargo-selective complex | CC |
| GO:0015031 | protein transport | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed