Gene loci information

Transcript annotation

  • This transcript has been annotated as Vacuolar protein sorting-associated protein 35.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g8055 g8055.t7 TSS g8055.t7 28278423 28278423
chr_2 g8055 g8055.t7 isoform g8055.t7 28279164 28279978
chr_2 g8055 g8055.t7 exon g8055.t7.exon1 28279164 28279978
chr_2 g8055 g8055.t7 cds g8055.t7.CDS1 28279198 28279977
chr_2 g8055 g8055.t7 TTS g8055.t7 NA NA

Sequences

>g8055.t7 Gene=g8055 Length=815
AAGCTGTAGAGATGCCATCGCACAAGAATATTTAATGGAATGCATTATTCAAGTGTTTCC
AGATGAATTTCATTTACAGACGCTTGATCCATTCCTCAAATCTTGTGCTCAACTACAATC
AGGAGTGAATGTTAAATCAATTATAATTTCACTTATCGACAGACTTGCTGCATATCATCA
ACGCAATGAAAAAGCTCACATTGATGCTGCTGATGGTTCAATCATACCAATTGATGTACA
GCTTTTTGATGTTTTTAGTATTCAGATTGCAAACATTGTGCAGATCAGAACTGACATGCC
GTTAGAAGACACAATCTCACTACAAGTTGCTCTCGTAAATCTTGCACAAAAGGTTTATCC
CGATCAAATCGATTATGTTGACAAAGTACTTGAGACAACAACACAAATTTTGGAGAATTT
AAATATGTCAAGCATCAACCATTCATTGGCTGTTAATCAAGAACTTTCTCGATTATTGAG
AGTTTGTATTGATTTCTATAATAACATTCTGACAATTTTAGATCTGAAATTTTTCCCAAA
ATTGCTTTCAAAATTCGACTACACATCACGAAAAGCACTTGCTCTGTATTTGGTCATGAA
TATTTTAGATAATGAGACTTTTATTCCAACTGATGAGCAAGTTGACGCTGTTCTTGAAAT
GTTATCACCATTGATTAAAGATCAAGAGGATCAGCCTGCTGATACATTTGACCCAGAAGA
TTTTGCAGAAGAGCAAGGAATTGTTGGACGTTTTATACATTTGCTCAAGTCTGAACATCT
TGATATGCAATATAAAATTTTAACTGTTGCGAAAA

>g8055.t7 Gene=g8055 Length=260
MECIIQVFPDEFHLQTLDPFLKSCAQLQSGVNVKSIIISLIDRLAAYHQRNEKAHIDAAD
GSIIPIDVQLFDVFSIQIANIVQIRTDMPLEDTISLQVALVNLAQKVYPDQIDYVDKVLE
TTTQILENLNMSSINHSLAVNQELSRLLRVCIDFYNNILTILDLKFFPKLLSKFDYTSRK
ALALYLVMNILDNETFIPTDEQVDAVLEMLSPLIKDQEDQPADTFDPEDFAEEQGIVGRF
IHLLKSEHLDMQYKILTVAK

Protein features from InterProScan

Transcript Database ID Name Start End E.value
2 g8055.t7 PANTHER PTHR11099 VACUOLAR SORTING PROTEIN 35 1 259 0
3 g8055.t7 PANTHER PTHR11099:SF0 VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 35 1 259 0
1 g8055.t7 Pfam PF03635 Vacuolar protein sorting-associated protein 35 1 260 0

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0042147 retrograde transport, endosome to Golgi BP
GO:0030906 retromer, cargo-selective complex CC
GO:0015031 protein transport BP

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values