General information for g8105.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable cytochrome P450 12a4, mitochondrial.
Parent gene
- The parent gene g8105 is located on chr_2: 28582518..28583171 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8105 | g8105.t1 | TTS | g8105.t1 | 28580827 | 28580827 |
| chr_2 | g8105 | g8105.t1 | isoform | g8105.t1 | 28581196 | 28583171 |
| chr_2 | g8105 | g8105.t1 | exon | g8105.t1.exon1 | 28581196 | 28581516 |
| chr_2 | g8105 | g8105.t1 | cds | g8105.t1.CDS1 | 28581196 | 28581516 |
| chr_2 | g8105 | g8105.t1 | exon | g8105.t1.exon2 | 28581571 | 28582086 |
| chr_2 | g8105 | g8105.t1 | cds | g8105.t1.CDS2 | 28581571 | 28582086 |
| chr_2 | g8105 | g8105.t1 | exon | g8105.t1.exon3 | 28582146 | 28582299 |
| chr_2 | g8105 | g8105.t1 | cds | g8105.t1.CDS3 | 28582146 | 28582299 |
| chr_2 | g8105 | g8105.t1 | exon | g8105.t1.exon4 | 28582709 | 28582825 |
| chr_2 | g8105 | g8105.t1 | cds | g8105.t1.CDS4 | 28582709 | 28582825 |
| chr_2 | g8105 | g8105.t1 | exon | g8105.t1.exon5 | 28582897 | 28583171 |
| chr_2 | g8105 | g8105.t1 | cds | g8105.t1.CDS5 | 28582897 | 28583171 |
| chr_2 | g8105 | g8105.t1 | TSS | g8105.t1 | 28583368 | 28583368 |
Sequences
>g8105.t1 Gene=g8105 Length=1383
ATGATTAGACATTTTGCAATACCTGGGGGAAAGTTTTACAATAAACCACTTAACGAATTA
CTTCAATTGTTAAGAAAGGAATATGGCGACATTTTTTACTTTCCGGGAATACTTGGTCAA
AAGCCGCTTATTATGACATTCAAACCAGAAGATGCAGAGAAAGTTTTTAGAGTTGAAGGT
CGCTGGCCGTACAGACGTCCTTTAGAAACCTTTACACATTACAGACGCAATTATAGACAA
GATATTTTTGAAAGTAGTGGAGGCTTAACAGTTGAACATGGTGAAAAATGGCATGATTTT
CGAACAAAAGTAAACCAAATCATGATGCAACCAAAAAACACGAAGATGTACATCCCAAAA
ATTGATGAAGTAACAATGGATTTCATTGATAAAATGCGTGCAATAAGGAACGAGAATAAT
GAAATGCCTGCTGATTTTTTTGAAACATTAAATGAATGGTCGCTTGAAAGTATAGCGCTT
ATAGCTCTAGATACGAGATTAGGGCTATTAAAAGGAAATAATCCTGAAGCACAAAAGCTC
TATGAATTGGTGAAAGAAGTTTTTACAGCCTCTTATGAATATGACATACAGCCCAGTATT
TGGAGATATTATCAAACACCAGGATTTAAGAAAGCAATGAAGTCTTTTGACGCTGTAACA
GATACGATTTATAAATATATCGAAAAAGCTCTAGAAAAATATAAAAAGAATCCAAATGAG
AATGAAAGTGGTGTTCTAGAAAAACTTTTAAAAATAGATAAAAATATTGCTATTGTCATG
GCAATGGATATGCTTGGAGCTGGAATCGATACAACATCTTCAAGTGTCGTTGCAAATCTG
TATTGCTTAGCAAAAAATCAAGATAAACAAGAAATTTTGCGTCAAGAAATTCTTAAAGTC
TTGCCTACAAAAGAAACCAAATTGGATACAAAATCCTTAGATAATGTGCCTTATTTACGT
GCAGTGATCAAAGAATCACTTCGAATGCATCCAATATTTAGTGGAAATTCTAGAGCAGTT
GATTGTGATTTAGTTTTACAAGGATATCAAATACCTAAAGGGATCGATATACTTCTTGTC
GCCAATTCACATAATGAGCATTTTGATCAAGCTGATAAATTTATTCCAGAGAGATGGCTA
AAAGATAATACCGATCCAAAATGTCCACATGCAAAAGATTCACATCCTTTTGCATTTTTG
CCTTTTGGATTCGCTTCACGTGCTTGTATCGGCAAAAGAATGGCAAATATGGAAATGGAA
GTTCTGATTGCAAGAGTGCTTCGGGAATTTAAAATTGAATGGAATCATTCAGATATAAAA
TGGAAAAGCGTTATTATCAACTATCCTGATTCACCTTTAAAATTTAAAGCAATTGATATT
TAA
>g8105.t1 Gene=g8105 Length=460
MIRHFAIPGGKFYNKPLNELLQLLRKEYGDIFYFPGILGQKPLIMTFKPEDAEKVFRVEG
RWPYRRPLETFTHYRRNYRQDIFESSGGLTVEHGEKWHDFRTKVNQIMMQPKNTKMYIPK
IDEVTMDFIDKMRAIRNENNEMPADFFETLNEWSLESIALIALDTRLGLLKGNNPEAQKL
YELVKEVFTASYEYDIQPSIWRYYQTPGFKKAMKSFDAVTDTIYKYIEKALEKYKKNPNE
NESGVLEKLLKIDKNIAIVMAMDMLGAGIDTTSSSVVANLYCLAKNQDKQEILRQEILKV
LPTKETKLDTKSLDNVPYLRAVIKESLRMHPIFSGNSRAVDCDLVLQGYQIPKGIDILLV
ANSHNEHFDQADKFIPERWLKDNTDPKCPHAKDSHPFAFLPFGFASRACIGKRMANMEME
VLIARVLREFKIEWNHSDIKWKSVIINYPDSPLKFKAIDI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g8105.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 4 | 459 | 0 |
| 2 | g8105.t1 | PANTHER | PTHR24305:SF194 | CYTOCHROME P450 12A4, MITOCHONDRIAL-RELATED | 8 | 456 | 0 |
| 3 | g8105.t1 | PANTHER | PTHR24305 | CYTOCHROME P450 | 8 | 456 | 0 |
| 8 | g8105.t1 | PRINTS | PR00463 | E-class P450 group I signature | 256 | 273 | 0 |
| 10 | g8105.t1 | PRINTS | PR00385 | P450 superfamily signature | 267 | 284 | 0 |
| 6 | g8105.t1 | PRINTS | PR00463 | E-class P450 group I signature | 320 | 338 | 0 |
| 9 | g8105.t1 | PRINTS | PR00385 | P450 superfamily signature | 321 | 332 | 0 |
| 4 | g8105.t1 | PRINTS | PR00463 | E-class P450 group I signature | 357 | 381 | 0 |
| 7 | g8105.t1 | PRINTS | PR00463 | E-class P450 group I signature | 399 | 409 | 0 |
| 11 | g8105.t1 | PRINTS | PR00385 | P450 superfamily signature | 400 | 409 | 0 |
| 5 | g8105.t1 | PRINTS | PR00463 | E-class P450 group I signature | 409 | 432 | 0 |
| 12 | g8105.t1 | PRINTS | PR00385 | P450 superfamily signature | 409 | 420 | 0 |
| 1 | g8105.t1 | Pfam | PF00067 | Cytochrome P450 | 15 | 439 | 0 |
| 13 | g8105.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 15 | 457 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
- This transcript has been annotated with K15004 (cytochrome P450 family 12 [EC:1.14.-.-])
Pathway
This gene does not belong to any pathways.
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
