General information for g8132.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cytochrome P450 4d2.
Parent gene
- The parent gene g8132 is located on chr_2: 28722381..28724549 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8132 | g8132.t1 | TSS | g8132.t1 | 28720199 | 28720199 |
| chr_2 | g8132 | g8132.t1 | isoform | g8132.t1 | 28720256 | 28722381 |
| chr_2 | g8132 | g8132.t1 | exon | g8132.t1.exon1 | 28720256 | 28720790 |
| chr_2 | g8132 | g8132.t1 | cds | g8132.t1.CDS1 | 28720256 | 28720790 |
| chr_2 | g8132 | g8132.t1 | exon | g8132.t1.exon2 | 28720849 | 28720918 |
| chr_2 | g8132 | g8132.t1 | cds | g8132.t1.CDS2 | 28720849 | 28720918 |
| chr_2 | g8132 | g8132.t1 | exon | g8132.t1.exon3 | 28720997 | 28721300 |
| chr_2 | g8132 | g8132.t1 | cds | g8132.t1.CDS3 | 28720997 | 28721300 |
| chr_2 | g8132 | g8132.t1 | exon | g8132.t1.exon4 | 28721377 | 28721392 |
| chr_2 | g8132 | g8132.t1 | cds | g8132.t1.CDS4 | 28721377 | 28721392 |
| chr_2 | g8132 | g8132.t1 | exon | g8132.t1.exon5 | 28721694 | 28721805 |
| chr_2 | g8132 | g8132.t1 | cds | g8132.t1.CDS5 | 28721694 | 28721805 |
| chr_2 | g8132 | g8132.t1 | exon | g8132.t1.exon6 | 28721872 | 28721978 |
| chr_2 | g8132 | g8132.t1 | cds | g8132.t1.CDS6 | 28721872 | 28721978 |
| chr_2 | g8132 | g8132.t1 | exon | g8132.t1.exon7 | 28722032 | 28722381 |
| chr_2 | g8132 | g8132.t1 | cds | g8132.t1.CDS7 | 28722032 | 28722381 |
| chr_2 | g8132 | g8132.t1 | TTS | g8132.t1 | 28722495 | 28722495 |
Sequences
>g8132.t1 Gene=g8132 Length=1494
ATGTTGATTGTAATTTTAACACTTACAATTGTACTTTTTTGGCTTTTAAGGGATGAATTT
AGAAAATTTTATTTAGCATTTTTTATTCCTGGTCCTCCAGGATTACCATTGGCAGGAAAT
GGATTAGATTTAATTAATAAATCACCACAAGAGTTGATTCAAATTATTTCGCGCTATTTA
AAAAATCATGGAAATTTTATTAGAATATGGCTTGGAAATCAACTCTTAATTGCAACTACA
GATCCAAAAGCCGTGCAAGCTATAATGACTGATAGCAATTTATTAACCAAATCGAGAGAA
TACGAATATATGGAAGCATGGCTTGGTACCGGTTTACTCACGTCAACAAATCAAAAGTGG
TTAAGTCGTCGTAAAATTCTTACACCTGCATTTCATTTTAAAATTCTAGATGAATTCATT
GAAGTTTTTGACAAGCAAGGCACAATTTTGATTGAAAAGCTAAAAAAATTTGATGGAAAA
GAATCATTTAATATATTTCCACTTGTTGCATTATGTGCTTTGGATGTCATTTGTGAAACT
GCTATGGGAATTTCACTCAATTCACAATCAGATTCAGAGTCGCAATATGTTAAAAATGTT
AATGAACTTGCCGATATTATACATCGTCGCACATATGATTTTATTGAACGATATGAATTT
TTGTTCAAATTCACCAAAACTTATAAACGACAGCAAAAGTTAGTTGAAGCTCTTCATCAG
TTCACGGAGTCAGTCATCAAATCACGTCGTAAAGAAATGAAGAGTAAACAAAATACTGCA
CAGGAAGACGAAGATTTTGGCATCAAGAAAAAGAAAGCTCTGCTTGATCTGTTACTAGAA
ACAACAGTTGAAGGAAAGCTATTGAGTAATGAAGACATTCGTGAGGAAATCGACACTTTT
ATGTTTGAGGGTCATGATACTTCAGCAAGTGCACTCGCTTATACACTTTATAACATTGCG
AGATATGAACACATTCAGAAAAAATGTTTTGAAGAAATTGACAGTGTGCTAGGAAATGAT
GGGAAAATTACTATGAAGTCACTTAATGAGTTGAAATATTTAGAACTTGTCTGCAAAGAA
TCATTGAGATTATATCCATCTGTTCCAATTTTTGGTCGAAGAACAGTTGAAGAAATGATG
ATAAATGATAAAATGATACCAAAAGATACAACTCTTCTAATGCTGCCTTACTTTATGGCA
AGAGATCCAGAAATTTGGCAAAATCCTGAAAAATTTATTCCTGAAAGACACGCTGTTGAA
AAGGATAACGAAGATTCGCAAGTTTTTACATACATTCCTTTCTCGGGTGGTTACCGAAAT
TGTATTGGACAAAAATTCGCTATGTTAGAACTTAAATCGACCGTCTCAAAAGTAGTATTG
AATTACAAATTAAGTGTAAAAGAAAATTTCACTCCTGAAGACGCTCTTGAACTTGTCATA
AAAAGTACTAATGGAATAATGCTCAAAATTGAAAGTAGAAAATTAAGCAATTAA
>g8132.t1 Gene=g8132 Length=497
MLIVILTLTIVLFWLLRDEFRKFYLAFFIPGPPGLPLAGNGLDLINKSPQELIQIISRYL
KNHGNFIRIWLGNQLLIATTDPKAVQAIMTDSNLLTKSREYEYMEAWLGTGLLTSTNQKW
LSRRKILTPAFHFKILDEFIEVFDKQGTILIEKLKKFDGKESFNIFPLVALCALDVICET
AMGISLNSQSDSESQYVKNVNELADIIHRRTYDFIERYEFLFKFTKTYKRQQKLVEALHQ
FTESVIKSRRKEMKSKQNTAQEDEDFGIKKKKALLDLLLETTVEGKLLSNEDIREEIDTF
MFEGHDTSASALAYTLYNIARYEHIQKKCFEEIDSVLGNDGKITMKSLNELKYLELVCKE
SLRLYPSVPIFGRRTVEEMMINDKMIPKDTTLLMLPYFMARDPEIWQNPEKFIPERHAVE
KDNEDSQVFTYIPFSGGYRNCIGQKFAMLELKSTVSKVVLNYKLSVKENFTPEDALELVI
KSTNGIMLKIESRKLSN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 16 | g8132.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 17 | 497 | 1.4E-121 |
| 2 | g8132.t1 | PANTHER | PTHR24291:SF143 | CYTOCHROME P450 4D1-RELATED | 5 | 493 | 3.8E-158 |
| 3 | g8132.t1 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 5 | 493 | 3.8E-158 |
| 8 | g8132.t1 | PRINTS | PR00463 | E-class P450 group I signature | 60 | 79 | 1.7E-18 |
| 10 | g8132.t1 | PRINTS | PR00385 | P450 superfamily signature | 303 | 320 | 4.4E-11 |
| 4 | g8132.t1 | PRINTS | PR00463 | E-class P450 group I signature | 312 | 338 | 1.7E-18 |
| 6 | g8132.t1 | PRINTS | PR00463 | E-class P450 group I signature | 355 | 373 | 1.7E-18 |
| 12 | g8132.t1 | PRINTS | PR00385 | P450 superfamily signature | 356 | 367 | 4.4E-11 |
| 5 | g8132.t1 | PRINTS | PR00463 | E-class P450 group I signature | 395 | 419 | 1.7E-18 |
| 9 | g8132.t1 | PRINTS | PR00463 | E-class P450 group I signature | 431 | 441 | 1.7E-18 |
| 11 | g8132.t1 | PRINTS | PR00385 | P450 superfamily signature | 432 | 441 | 4.4E-11 |
| 7 | g8132.t1 | PRINTS | PR00463 | E-class P450 group I signature | 441 | 464 | 1.7E-18 |
| 13 | g8132.t1 | PRINTS | PR00385 | P450 superfamily signature | 441 | 452 | 4.4E-11 |
| 1 | g8132.t1 | Pfam | PF00067 | Cytochrome P450 | 30 | 488 | 6.4E-110 |
| 18 | g8132.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 17 | - |
| 19 | g8132.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 1 | - |
| 20 | g8132.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 2 | 13 | - |
| 21 | g8132.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 14 | 17 | - |
| 17 | g8132.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 18 | 497 | - |
| 15 | g8132.t1 | ProSitePatterns | PS00086 | Cytochrome P450 cysteine heme-iron ligand signature. | 434 | 443 | - |
| 14 | g8132.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 29 | 493 | 1.26E-114 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
