Gene loci information

Transcript annotation

  • This transcript has been annotated as Probable cytochrome P450 12b2, mitochondrial.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g8133 g8133.t1 TTS g8133.t1 28722419 28722419
chr_2 g8133 g8133.t1 isoform g8133.t1 28722513 28724549
chr_2 g8133 g8133.t1 exon g8133.t1.exon1 28722513 28722687
chr_2 g8133 g8133.t1 cds g8133.t1.CDS1 28722513 28722687
chr_2 g8133 g8133.t1 exon g8133.t1.exon2 28722746 28723143
chr_2 g8133 g8133.t1 cds g8133.t1.CDS2 28722746 28723143
chr_2 g8133 g8133.t1 exon g8133.t1.exon3 28723197 28723353
chr_2 g8133 g8133.t1 cds g8133.t1.CDS3 28723197 28723353
chr_2 g8133 g8133.t1 exon g8133.t1.exon4 28723412 28723527
chr_2 g8133 g8133.t1 cds g8133.t1.CDS4 28723412 28723527
chr_2 g8133 g8133.t1 exon g8133.t1.exon5 28723581 28723857
chr_2 g8133 g8133.t1 cds g8133.t1.CDS5 28723581 28723857
chr_2 g8133 g8133.t1 exon g8133.t1.exon6 28723924 28724036
chr_2 g8133 g8133.t1 cds g8133.t1.CDS6 28723924 28724036
chr_2 g8133 g8133.t1 exon g8133.t1.exon7 28724108 28724204
chr_2 g8133 g8133.t1 cds g8133.t1.CDS7 28724108 28724204
chr_2 g8133 g8133.t1 exon g8133.t1.exon8 28724268 28724304
chr_2 g8133 g8133.t1 cds g8133.t1.CDS8 28724268 28724304
chr_2 g8133 g8133.t1 exon g8133.t1.exon9 28724366 28724549
chr_2 g8133 g8133.t1 cds g8133.t1.CDS9 28724366 28724549
chr_2 g8133 g8133.t1 TSS g8133.t1 28724569 28724569

Sequences

>g8133.t1 Gene=g8133 Length=1554
ATGATTTGCAACATTTTGAAGTTTAATAAATTGAGTAATTTGCAAAAGTCATTTGCTTAT
TTTTCCACACAAGTGGCCAAAAAAGAATTTGTTAATTTTCAAGATGAGTGGGAAAATGCA
AGATCATTTGATGAAATTCCTCAACCATCGAAAATTTCATTAATTCGTGCATTTTTACCT
GGAGGAAAATATTACAAACTATCGTTAATTGATATGCATAGATCAATGAACAAAGAATAT
GGAACAATGGTTAAATTTCCTGAAGTTCTTGGAAGACGTGGAATGGTTTGCAGTTATAAT
GTCGAAGATGTAGAAAAGCTGTTTAGACATGAAGGTCAAACGCCTTATCGTAGAACACTC
GAAACATTGCATCATTATAGAAAAAAATATCGACCTGAAGTTTACGAAGAATATGGAGGT
TTGTTTACAGAACAAGGCGAGTCATGGTATAAGATGCGTACGATTGCTAATCCTGTACTG
TTACAACCAAAAACAATTAAGCAGTACATTCCACAGGTTGATGATGTTTCGAAAGAATTT
ATAAAAATGATCGTTCAAAAACTTGATGCCAACAATGAATGTCCTGAAAATTTCAGTGAT
TATTTGAATTTGTGGAGTCTAGAGAGCATTGCTAATATTTCTCTCGATAGGCGTCTTGGT
ATTGTTAGTGGAAATTATCAAGACGAAATGGCAGAAAGACTCATAAAATCAATTAGACAA
TTCTTTGTTGAAGTCTATGAATTTGAAGTTAATATGTCGGTTTGGAAATATTATGAAACG
AAGGCATTTAAAAATCTGATGAACCTTTATGATGAACTTACCAACATTATATTACATTAT
GTTGAGAAAGCCATTGAAGATATTCAAAAGAAAACTATTCGAAATGACAAAGAAGAAAGT
ATTTTGGAAAAATTGTTAAAGATAAATAAAAATGCTGCCATAGTCATGACTGCTGATGCT
CTTTTAGCTGGTGTTGATACGACAAGCTCAGCAACAATAGGCATTTTATATTGCTTGGCA
AAAAATCAAGATAAACAAAACAAATTACGGGAAGAATTGAAATCAATATTAAGGAATGAA
AATGATGAATTGACTCCAAAAAATATGCAAAACATGCCTTATTTACGAGCATGCATTAAA
GAAGGATTTCGACTTTATCCACCAACAGTAGGAACTGTGAGAAAAGCTGATCAAGATTTA
GTCTTGTCTGGTTATAAAATTCCGAAAGGAACTGAAGTTGTTATGCCTATAGCTTTATTT
CAAAAAGATTGCTATACACGTGCAAATGAATTCATTCCAGAACGTTGGTTGAAAGAAATT
TTAGACACAAATTGTCCTCATGCAAAAGAAGCTTCACCATTTTCTTATCTTCCATTTGGG
TTCGGTTCACGAGTATGTATCGGAAAAAGATTGGCTAATCTTGAAATGGAAGTTCTAATA
ACTAGGCTTATTCGTGATTATAAAATAGAATGGAATTATCCTGAATTGAAATTCAAAGTT
GCTATTGTGAATATACCTGATGGTGCATTAAAGTTTAAAATTACTAAAATATAA

>g8133.t1 Gene=g8133 Length=517
MICNILKFNKLSNLQKSFAYFSTQVAKKEFVNFQDEWENARSFDEIPQPSKISLIRAFLP
GGKYYKLSLIDMHRSMNKEYGTMVKFPEVLGRRGMVCSYNVEDVEKLFRHEGQTPYRRTL
ETLHHYRKKYRPEVYEEYGGLFTEQGESWYKMRTIANPVLLQPKTIKQYIPQVDDVSKEF
IKMIVQKLDANNECPENFSDYLNLWSLESIANISLDRRLGIVSGNYQDEMAERLIKSIRQ
FFVEVYEFEVNMSVWKYYETKAFKNLMNLYDELTNIILHYVEKAIEDIQKKTIRNDKEES
ILEKLLKINKNAAIVMTADALLAGVDTTSSATIGILYCLAKNQDKQNKLREELKSILRNE
NDELTPKNMQNMPYLRACIKEGFRLYPPTVGTVRKADQDLVLSGYKIPKGTEVVMPIALF
QKDCYTRANEFIPERWLKEILDTNCPHAKEASPFSYLPFGFGSRVCIGKRLANLEMEVLI
TRLIRDYKIEWNYPELKFKVAIVNIPDGALKFKITKI

Protein features from InterProScan

Transcript Database ID Name Start End E.value
15 g8133.t1 Coils Coil Coil 339 359 -
14 g8133.t1 Gene3D G3DSA:1.10.630.10 Cytochrome p450 35 517 1.2E-118
2 g8133.t1 PANTHER PTHR24291:SF155 CYTOCHROME P450 301B1 38 500 5.1E-112
3 g8133.t1 PANTHER PTHR24291 CYTOCHROME P450 FAMILY 4 38 500 5.1E-112
7 g8133.t1 PRINTS PR00385 P450 superfamily signature 323 340 7.9E-11
11 g8133.t1 PRINTS PR00465 E-class P450 group IV signature 372 388 6.4E-10
4 g8133.t1 PRINTS PR00385 P450 superfamily signature 377 388 7.9E-11
10 g8133.t1 PRINTS PR00465 E-class P450 group IV signature 419 437 6.4E-10
9 g8133.t1 PRINTS PR00465 E-class P450 group IV signature 450 466 6.4E-10
6 g8133.t1 PRINTS PR00385 P450 superfamily signature 457 466 7.9E-11
5 g8133.t1 PRINTS PR00385 P450 superfamily signature 466 477 7.9E-11
8 g8133.t1 PRINTS PR00465 E-class P450 group IV signature 466 484 6.4E-10
1 g8133.t1 Pfam PF00067 Cytochrome P450 71 491 1.0E-76
13 g8133.t1 ProSitePatterns PS00086 Cytochrome P450 cysteine heme-iron ligand signature. 459 468 -
12 g8133.t1 SUPERFAMILY SSF48264 Cytochrome P450 47 515 2.75E-82

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0004497 monooxygenase activity MF
GO:0055114 NA NA

KEGG

Orthology

Pathway

This gene does not belong to any pathways.

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values