General information for g8209.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative eIF-2-alpha kinase GCN2.
Parent gene
- The parent gene g8209 is located on chr_2: 29068433..29069206 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8209 | g8209.t3 | isoform | g8209.t3 | 29068433 | 29069206 |
| chr_2 | g8209 | g8209.t3 | exon | g8209.t3.exon1 | 29068433 | 29069206 |
| chr_2 | g8209 | g8209.t3 | cds | g8209.t3.CDS1 | 29068434 | 29069036 |
| chr_2 | g8209 | g8209.t3 | TSS | g8209.t3 | 29069314 | 29069314 |
| chr_2 | g8209 | g8209.t3 | TTS | g8209.t3 | NA | NA |
Sequences
>g8209.t3 Gene=g8209 Length=774
ATGACAACTAAAGAAAGCTATAAAGACAGACAAGAAAATGAATTAGAAGTGTTGAAGGTA
TTTGTGAGCAATTATGTGAAAAAATTTCGTCACTTAAAATATTTTTTGTGGGTTTTTAGT
CAATATTTGGTGATGACGTTGTTGATTTGCGGAAGAAAGAGGAAAAATGGATGCCACTTG
ATTTAACAATAAAGCTTAAACCTCAGAGAGATTCAAAAATATCTCAGGCCTATTGTCATA
TTGAACTTCATGTAATATGCCCGAAGAATTATCCAAAAGTTTGTCCCAAGCTTTCTTTAG
AAAATGCAAAAGGTTTAGCAAATTCTGATGTTGTTACACTCCTCAATCAAATCAATAAGG
AGGCATATGGTTTAAAAGGAGAAGTTATGATTTTCGAGATATGTCAATTTATTCAGTCAT
TTTTGCATGAACGCAATGTGCCGCCTAAAGGTTCATTTTATGATGAAATGCTAGCTACAA
AGCAGAAAAGAGATCAAGATGAACTTACACAGCAAAAAGAAATAGAAGAAAAGAAACGAC
AACACATACAGGATGAAATTCTCAAGAGAAAAGAGCAATTGAGAAAAGAAAATCGAATAC
GAAAAGGTACTACTACTGATAGTCCGTTACATCATATTTCTTCAGAAAGTTCCAATTCCG
ATTCTAAATTTGTCAACGAGATTTGTGATGAACATAACTGTTCGGAGAATCTCTATTTTC
CAAATGATGGTAGAAAGATATTAAAGGGGACATGTATAGGACATTCAATGAAAG
>g8209.t3 Gene=g8209 Length=201
MPLDLTIKLKPQRDSKISQAYCHIELHVICPKNYPKVCPKLSLENAKGLANSDVVTLLNQ
INKEAYGLKGEVMIFEICQFIQSFLHERNVPPKGSFYDEMLATKQKRDQDELTQQKEIEE
KKRQHIQDEILKRKEQLRKENRIRKGTTTDSPLHHISSESSNSDSKFVNEICDEHNCSEN
LYFPNDGRKILKGTCIGHSMK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g8209.t3 | Coils | Coil | Coil | 102 | 129 | - |
| 5 | g8209.t3 | Gene3D | G3DSA:3.10.110.10 | Ubiquitin Conjugating Enzyme | 1 | 97 | 1.4E-24 |
| 8 | g8209.t3 | MobiDBLite | mobidb-lite | consensus disorder prediction | 138 | 165 | - |
| 9 | g8209.t3 | MobiDBLite | mobidb-lite | consensus disorder prediction | 150 | 165 | - |
| 2 | g8209.t3 | PANTHER | PTHR11042 | EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED | 28 | 155 | 5.3E-17 |
| 3 | g8209.t3 | PANTHER | PTHR11042:SF164 | EIF-2-ALPHA KINASE GCN2 | 28 | 155 | 5.3E-17 |
| 1 | g8209.t3 | Pfam | PF05773 | RWD domain | 4 | 84 | 2.6E-14 |
| 10 | g8209.t3 | ProSiteProfiles | PS50908 | RWD domain profile. | 1 | 88 | 17.401 |
| 7 | g8209.t3 | SMART | SM00591 | RWD2001b | 1 | 88 | 3.8E-10 |
| 4 | g8209.t3 | SUPERFAMILY | SSF54495 | UBC-like | 2 | 94 | 3.87E-19 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005515 | protein binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
