General information for g8216.t6
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as V-type proton ATPase catalytic subunit A isoform 2.
Parent gene
- The parent gene g8216 is located on chr_2: 29095694..29099327 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8216 | g8216.t6 | isoform | g8216.t6 | 29093613 | 29096122 |
| chr_2 | g8216 | g8216.t6 | exon | g8216.t6.exon1 | 29093613 | 29093764 |
| chr_2 | g8216 | g8216.t6 | TSS | g8216.t6 | 29093634 | 29093634 |
| chr_2 | g8216 | g8216.t6 | exon | g8216.t6.exon2 | 29094052 | 29094134 |
| chr_2 | g8216 | g8216.t6 | exon | g8216.t6.exon3 | 29094215 | 29094409 |
| chr_2 | g8216 | g8216.t6 | exon | g8216.t6.exon4 | 29094472 | 29095559 |
| chr_2 | g8216 | g8216.t6 | cds | g8216.t6.CDS1 | 29095213 | 29095559 |
| chr_2 | g8216 | g8216.t6 | exon | g8216.t6.exon5 | 29095633 | 29096122 |
| chr_2 | g8216 | g8216.t6 | cds | g8216.t6.CDS2 | 29095633 | 29096122 |
| chr_2 | g8216 | g8216.t6 | TTS | g8216.t6 | 29096588 | 29096588 |
Sequences
>g8216.t6 Gene=g8216 Length=2008
TGTATGATACTAAAACATCAGATAAGGTTGTCAAGTGACCGAGTGAGCGACAGTGGAGCA
ACATTCTGAAAAATTATTTTGAAAAGGAAAAAGAATAGTTTTTCAAGATATTTTAAAGAG
AATATTTATTGTGAGCATCAACATTAACCAATCGAGAAGATGTCTAATTTAAAGAAGATA
GCAAATGAGGACCGTGAGTCAAAATTCGGCTATGTATTTGCCGTTTCAGGACCTGTCGTC
ACTGCTGAACGAATGGCTGGATCAGCTATGTACGAGTTGGTGCGTGTAGGCTATTATGAA
TTGGTTGGTGAAATTATTCGTTTAGAAGGTGATATGGCAACAATTCAAGTCTATGAAGAA
ACATCAGGTGTAACTGTTGGTGATCCTGTCTTGAGAACTGGCAAACCTTTATCAGTCGAA
TTGGGTCCAGGTATTATGGGTAGTATTTTTGACGGTATTCAACGTCCATTAAAAGACATT
AATGAATTGACACAATCAATTTATATCCCAAAGGGAATTAATATTCCTTCACTTTCTCGT
ACTCAATCATGGGATTTTAATCCATTAAATGTAAAAGTCGGATCGCACATAACTGGAGGT
GATTTGTATGGTGTAGTTCATGAAAATACTTTAGTCAAACATAAATTGATTGTACCACCG
AGAGCGAAAGGAACAGTTCGTTATATTGCACCACCAGGCAATTATACAGTGGATGATATC
ATTCTTGAGACAGAATTTGATGGTGAAATCAATAAATATTCCATGTTGCAAGTGTGGCCT
GTTCGTCAGCCACGTCCAGTCACTGAAAAATTGCCAGCAAATCATCCCTTGCTGACTGGT
CAGCGTGTCTTGGATTCATTGTTTCCATGTGTTCAAGGAGGAACAACTGCTATACCTGGT
GCTTTCGGTTGTGAAAAAAACTGTTATTTTCGCAAGCCCTTTCAAAATATTCCAATTCAG
ATGTCATTATTTATGTCGGTTGCGGAGAACGTGGAAATGAAATGTCTGAAGTACTTCGTG
ATTTCCCAAGATTGTCTGTTGAAATTGATGGCGTAACTGAGTCTATCATGAAGCGTACAG
CTCTTGTCGCTAACACATCAAACATGCCTGTTGCTCTGCTCGTGAAGCTTCCATTTATAC
TGGTATTACACTTTCTGAATACTTCCGTGATATGGGTTATAATGTCTCTATGATGGCTGA
TTCGACTTCTCGTTGGGCTGAGGCTCTTCGTGAAATTTCAGGTCGTCTCGCTGAAATGCC
TGCTGATTCAGGTTATCCAGCTTATTTGGGAGCTCGTCTTGCCTCATTTTACGAACGTGC
TGGTCGTGTTAAATGTTTGGGTAATCCAGAACGTGAAGGTTCAGTATCAATTGTCGGCGC
TGTATCGCCACCTGGCGGTGACTTCTCAGATCCTGTTACATCTGCTACTTTAGGTATTGT
TCAAGTGTTTTGGGGTTTGGATAAAAAATTGGCCCAACGTAAACATTTTCCATCTATCAA
TTGGCTCATTTCATACAGTAAATATATGCGTGCACTTGATGATTTTTATGATAAAAACTT
CCCCGAATTCGTTCCATTACGTACAAAAGTGAAGGAAATTCTTCAAGAAGAAGAAGACTT
GTCAGAAATTGTGCAACTCGTTGGTAAAGCATCATTGGCTGAAACTGATAAGATCACATT
AGAAGTTGCTAAGCTTCTTAAGGATGACTTTTTACAACAAAATTCTTATTCATCATATGA
TCGTTTCTGTCCATTCTATAAAACTGTCGGCATGTTAAGAAATTTTATTGCATTCTACGA
TATGGCTCGTCACGCTGTTGAATCAACAGCACAATCAGAAAATAAAATTACATGGAACAT
CATTCGTGATTCAATGGGCAACATGCTTTATCAATTATCATCTATGAAATTTAAAGATCC
AGTCAAAGACGGTGAAGCAAAAATCAAAGCTGATTTCGATCAATTGCATGAAGAATTACA
ACAAGCTTTCCGCAATCTAGAGGATTAA
>g8216.t6 Gene=g8216 Length=278
MGYNVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPE
REGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSINWLISYSKYMR
ALDDFYDKNFPEFVPLRTKVKEILQEEEDLSEIVQLVGKASLAETDKITLEVAKLLKDDF
LQQNSYSSYDRFCPFYKTVGMLRNFIAFYDMARHAVESTAQSENKITWNIIRDSMGNMLY
QLSSMKFKDPVKDGEAKIKADFDQLHEELQQAFRNLED
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g8216.t6 | CDD | cd18111 | ATP-synt_V_A-type_alpha_C | 133 | 238 | 3.1999E-52 |
| 9 | g8216.t6 | Coils | Coil | Coil | 259 | 278 | - |
| 7 | g8216.t6 | Gene3D | G3DSA:3.40.50.300 | - | 1 | 116 | 4.1E-50 |
| 8 | g8216.t6 | Gene3D | G3DSA:1.10.1140.10 | - | 117 | 276 | 2.8E-52 |
| 2 | g8216.t6 | PANTHER | PTHR43607:SF1 | V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A | 1 | 276 | 2.6E-145 |
| 3 | g8216.t6 | PANTHER | PTHR43607 | V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A | 1 | 276 | 2.6E-145 |
| 1 | g8216.t6 | Pfam | PF00006 | ATP synthase alpha/beta family, nucleotide-binding domain | 1 | 116 | 7.1E-55 |
| 6 | g8216.t6 | ProSitePatterns | PS00152 | ATP synthase alpha and beta subunits signature. | 107 | 116 | - |
| 4 | g8216.t6 | SUPERFAMILY | SSF52540 | P-loop containing nucleoside triphosphate hydrolases | 1 | 121 | 5.01E-37 |
| 5 | g8216.t6 | SUPERFAMILY | SSF47917 | C-terminal domain of alpha and beta subunits of F1 ATP synthase | 131 | 233 | 8.89E-27 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0046034 | ATP metabolic process | BP |
| GO:0005524 | ATP binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
