General information for g8273.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha.
Parent gene
- The parent gene g8273 is located on chr_2: 29434780..29435490 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8273 | g8273.t1 | TSS | g8273.t1 | 29428873 | 29428873 |
| chr_2 | g8273 | g8273.t1 | isoform | g8273.t1 | 29429047 | 29435408 |
| chr_2 | g8273 | g8273.t1 | exon | g8273.t1.exon1 | 29429047 | 29429167 |
| chr_2 | g8273 | g8273.t1 | cds | g8273.t1.CDS1 | 29429047 | 29429167 |
| chr_2 | g8273 | g8273.t1 | exon | g8273.t1.exon2 | 29429897 | 29429931 |
| chr_2 | g8273 | g8273.t1 | cds | g8273.t1.CDS2 | 29429897 | 29429931 |
| chr_2 | g8273 | g8273.t1 | exon | g8273.t1.exon3 | 29429990 | 29430169 |
| chr_2 | g8273 | g8273.t1 | cds | g8273.t1.CDS3 | 29429990 | 29430169 |
| chr_2 | g8273 | g8273.t1 | exon | g8273.t1.exon4 | 29430350 | 29430547 |
| chr_2 | g8273 | g8273.t1 | cds | g8273.t1.CDS4 | 29430350 | 29430547 |
| chr_2 | g8273 | g8273.t1 | exon | g8273.t1.exon5 | 29430611 | 29431266 |
| chr_2 | g8273 | g8273.t1 | cds | g8273.t1.CDS5 | 29430611 | 29431266 |
| chr_2 | g8273 | g8273.t1 | exon | g8273.t1.exon6 | 29431346 | 29431450 |
| chr_2 | g8273 | g8273.t1 | cds | g8273.t1.CDS6 | 29431346 | 29431450 |
| chr_2 | g8273 | g8273.t1 | exon | g8273.t1.exon7 | 29431519 | 29431651 |
| chr_2 | g8273 | g8273.t1 | cds | g8273.t1.CDS7 | 29431519 | 29431651 |
| chr_2 | g8273 | g8273.t1 | exon | g8273.t1.exon8 | 29431721 | 29431808 |
| chr_2 | g8273 | g8273.t1 | cds | g8273.t1.CDS8 | 29431721 | 29431808 |
| chr_2 | g8273 | g8273.t1 | exon | g8273.t1.exon9 | 29434780 | 29434863 |
| chr_2 | g8273 | g8273.t1 | cds | g8273.t1.CDS9 | 29434780 | 29434863 |
| chr_2 | g8273 | g8273.t1 | exon | g8273.t1.exon10 | 29435356 | 29435408 |
| chr_2 | g8273 | g8273.t1 | cds | g8273.t1.CDS10 | 29435356 | 29435408 |
| chr_2 | g8273 | g8273.t1 | TTS | g8273.t1 | NA | NA |
Sequences
>g8273.t1 Gene=g8273 Length=1653
ATGGCTACAGTAGAAAAAATTGATAAAATTGAAAAGATTCAGCCTCACGACGATGTTTCA
ACTTCAAGAGCCTCAGAACAAACTGGAACAACAAAAGAGTCCGAAACAGCATCGGAAAGT
GCGAGAACTGCTGGAGATAGGAGTGCCATAAATGAGACGTTATCAGATGGCACCAGAGGA
AGTGGTGTCAACAGCTCGATGGCTGCAAGGACAGCAAAATCTGACAAAGAAAAGAAAATT
GGTCATCGAAGGGTTGGTGAAGATGGTGAAATCACTTATAAAAAGATCCAAACGAGCACT
ATTATGGGCTCAATTCAATTAGGCATACAACACACGGTTGGAAGTTTAGCCTCCAAACCG
AAACGTGATTTGCTAATGATGGACTTCTGGGAAATGGAAACAATTTCATTTCCGCCTGAA
GGATCAAGTATGACACCCGCTCATCATTATAGTGAATTTAGATTCAAAATTTATGCTCCT
ATCGCCTTCCGGTATTTTCGAGATTTATTTGGAATACAACCAGACGATTTTATGATGTCT
ATGTGTTCAGCACCACTTCGTGAACTCTCAAATCCAGGTGCATCTGGTTCTATATTTTAT
CTCACTGACGACGATGAGTTTATAATCAAAACAGTTCAACATAAAGAGGGAGAATTTTTG
CAAAAACTGCTTCCAGGCTACTATATGAATCTTAATCAAAATCCTCGTACACTATTACCA
AAATTTTTCGGTTTATATTGTTATCAATGTAATAGTAAAAATGTCCGTCTTGTTGCAATG
AATAATTTGTTACCGTCATCAATAAAGCTGCATTTGAAATATGATTTAAAAGGATCAACT
TATAAGCGAAAAGCAAATAAATCTGAACGTCAGAAAAAATCTCCCACTTATAAGGATTTA
GATTTTATGGAACATCATCCGAATGGTATTAGTTTGGAAGCTGACACGTATGCAGCATTG
ATAAAGACAATACAGAGGGATTGTCGTGTGTTAGAATCATTTAAAATTATGGATTATTCA
CTGCTCGTTGGAGTACACAATATGGATCAAGCTCAAAAGGAAAAGCAGGAAGAAAAACTA
AAGAACCCTCGCAAAGATTCACATGGTGATGATTCTGATATGGAAGACGCACCTGAAGCA
GATCAAGCGATGCATGAAGATAGGGCAGGATCTGCATTGAATCGTACAAGTGCATTTCAC
AGATCGATTAATCGTCAAAGATTAGTTGCCCATTCCACCGCAATGGAAAGCATTCAAGCG
GAAAGCGAACCAATTGATGAAGAGGAGGACGTTCCTCCCGGCGGCATTCCAGCACGAAAT
GATAAAGGAGAACGATTGCTTTTATTCATTGGTATTATTGATATTTTACAATCGTATCGT
TTAAAAAAGAAATTAGAGCATACATTCAAGAGCATAATTCACGATGGTGATACTGTTTCG
GTGCATCGTCCATCTTTTTATGCTCAACGTTTTCAAGATTTCATGGCCAAGACTGTATTT
CGGAAAATTCCATCGCCTCTCAAACATTCACCGTCGAAACGAAAAAGTTTATCAAAAGTC
GCACATCAAAGATCAACAGAATCTGAAGCTGACAGTCATGCGGCAAGCAGTTCACATAAT
CAGCCTCATAGTAGCTCTTCAAAAGCAGGTTAG
>g8273.t1 Gene=g8273 Length=550
MATVEKIDKIEKIQPHDDVSTSRASEQTGTTKESETASESARTAGDRSAINETLSDGTRG
SGVNSSMAARTAKSDKEKKIGHRRVGEDGEITYKKIQTSTIMGSIQLGIQHTVGSLASKP
KRDLLMMDFWEMETISFPPEGSSMTPAHHYSEFRFKIYAPIAFRYFRDLFGIQPDDFMMS
MCSAPLRELSNPGASGSIFYLTDDDEFIIKTVQHKEGEFLQKLLPGYYMNLNQNPRTLLP
KFFGLYCYQCNSKNVRLVAMNNLLPSSIKLHLKYDLKGSTYKRKANKSERQKKSPTYKDL
DFMEHHPNGISLEADTYAALIKTIQRDCRVLESFKIMDYSLLVGVHNMDQAQKEKQEEKL
KNPRKDSHGDDSDMEDAPEADQAMHEDRAGSALNRTSAFHRSINRQRLVAHSTAMESIQA
ESEPIDEEEDVPPGGIPARNDKGERLLLFIGIIDILQSYRLKKKLEHTFKSIIHDGDTVS
VHRPSFYAQRFQDFMAKTVFRKIPSPLKHSPSKRKSLSKVAHQRSTESEADSHAASSSHN
QPHSSSSKAG
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g8273.t1 | CDD | cd17301 | PIPKc_PIP5KI | 99 | 503 | 0.0 |
| 7 | g8273.t1 | Gene3D | G3DSA:3.30.800.10 | Phosphatidylinositol Phosphate Kinase II Beta | 95 | 262 | 2.2E-67 |
| 6 | g8273.t1 | Gene3D | G3DSA:3.30.810.10 | - | 263 | 372 | 1.6E-33 |
| 5 | g8273.t1 | Gene3D | G3DSA:3.30.810.10 | - | 373 | 503 | 3.3E-26 |
| 13 | g8273.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 19 | - |
| 17 | g8273.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 83 | - |
| 12 | g8273.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 20 | 70 | - |
| 14 | g8273.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 350 | 396 | - |
| 15 | g8273.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 350 | 388 | - |
| 11 | g8273.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 414 | 438 | - |
| 10 | g8273.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 505 | 550 | - |
| 16 | g8273.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 530 | 550 | - |
| 2 | g8273.t1 | PANTHER | PTHR23086:SF101 | LP03320P-RELATED | 32 | 514 | 7.6E-258 |
| 3 | g8273.t1 | PANTHER | PTHR23086 | PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE | 32 | 514 | 7.6E-258 |
| 1 | g8273.t1 | Pfam | PF01504 | Phosphatidylinositol-4-phosphate 5-Kinase | 184 | 497 | 3.6E-94 |
| 18 | g8273.t1 | ProSiteProfiles | PS51455 | Phosphatidylinositol phosphate kinase (PIPK) domain profile. | 97 | 499 | 68.216 |
| 9 | g8273.t1 | SMART | SM00330 | PIPK_2 | 125 | 500 | 7.5E-167 |
| 4 | g8273.t1 | SUPERFAMILY | SSF56104 | SAICAR synthase-like | 94 | 499 | 1.01E-118 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016307 | phosphatidylinositol phosphate kinase activity | MF |
| GO:0046488 | phosphatidylinositol metabolic process | BP |
KEGG
Orthology
- This transcript has been annotated with K00889 (1-phosphatidylinositol-4-phosphate 5-kinase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
