General information for g8326.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Histone H4.
Parent gene
- The parent gene g8326 is located on chr_2: 29781564..29781869 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8326 | g8326.t1 | isoform | g8326.t1 | 29781398 | 29781869 |
| chr_2 | g8326 | g8326.t1 | exon | g8326.t1.exon1 | 29781398 | 29781419 |
| chr_2 | g8326 | g8326.t1 | cds | g8326.t1.CDS1 | 29781398 | 29781419 |
| chr_2 | g8326 | g8326.t1 | exon | g8326.t1.exon2 | 29781565 | 29781869 |
| chr_2 | g8326 | g8326.t1 | cds | g8326.t1.CDS2 | 29781565 | 29781869 |
| chr_2 | g8326 | g8326.t1 | TTS | g8326.t1 | 29781988 | 29781988 |
| chr_2 | g8326 | g8326.t1 | TSS | g8326.t1 | NA | NA |
Sequences
>g8326.t1 Gene=g8326 Length=327
ATGGCTCCAGGCTTAGGAAAAGGTGGCAAGGGATTAGGCAAAGGCCTAGGAAAAGGAGGT
GCAAAGCGTCATCGCAAAGTTCTTCGTGATAACATCCAAGGTATTACAAAACCAGCAATT
CGTCGCTTAGCTCGTCGTGGTGGTGTCAAGCGTATCTCAGGCTTAATTTATGAAGAAACT
CGTGGCGTTTTAAAAATCTTTTTGGAAAATGTTATTCGTGATGCAGTCACATACACTGAA
CATGCCAAACGCAAGACAGTTACAGCTATGGATGTCGTTTATGCCTTGAAACGTCAAGGA
CGCACTCTCTATGGTTTCGGAGGATAA
>g8326.t1 Gene=g8326 Length=108
MAPGLGKGGKGLGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEET
RGVLKIFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g8326.t1 | CDD | cd00076 | H4 | 22 | 106 | 2.50614E-44 |
| 11 | g8326.t1 | Gene3D | G3DSA:1.10.20.10 | Histone | 4 | 108 | 2.0E-58 |
| 16 | g8326.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 24 | - |
| 2 | g8326.t1 | PANTHER | PTHR10484 | HISTONE H4 | 4 | 108 | 2.7E-64 |
| 3 | g8326.t1 | PANTHER | PTHR10484:SF185 | HISTONE H4 | 4 | 108 | 2.7E-64 |
| 6 | g8326.t1 | PRINTS | PR00623 | Histone H4 signature | 6 | 17 | 4.5E-61 |
| 7 | g8326.t1 | PRINTS | PR00623 | Histone H4 signature | 25 | 44 | 4.5E-61 |
| 9 | g8326.t1 | PRINTS | PR00623 | Histone H4 signature | 45 | 65 | 4.5E-61 |
| 5 | g8326.t1 | PRINTS | PR00623 | Histone H4 signature | 67 | 81 | 4.5E-61 |
| 8 | g8326.t1 | PRINTS | PR00623 | Histone H4 signature | 82 | 94 | 4.5E-61 |
| 4 | g8326.t1 | PRINTS | PR00623 | Histone H4 signature | 94 | 105 | 4.5E-61 |
| 1 | g8326.t1 | Pfam | PF15511 | Centromere kinetochore component CENP-T histone fold | 41 | 101 | 3.1E-8 |
| 13 | g8326.t1 | ProSitePatterns | PS00047 | Histone H4 signature. | 20 | 24 | - |
| 15 | g8326.t1 | SMART | SM00417 | h44 | 21 | 95 | 2.6E-29 |
| 14 | g8326.t1 | SMART | SM00803 | TAF_cls | 33 | 98 | 0.0061 |
| 10 | g8326.t1 | SUPERFAMILY | SSF47113 | Histone-fold | 9 | 107 | 1.07E-31 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0003677 | DNA binding | MF |
| GO:0046982 | protein heterodimerization activity | MF |
| GO:0006352 | DNA-templated transcription, initiation | BP |
| GO:0000786 | nucleosome | CC |
KEGG
Orthology
- This transcript has been annotated with K11254 (histone H4)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
