General information for g8359.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Dynactin subunit 2.
Parent gene
- The parent gene g8359 is located on chr_2: 30026640..30027967 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8359 | g8359.t2 | isoform | g8359.t2 | 30026184 | 30027967 |
| chr_2 | g8359 | g8359.t2 | exon | g8359.t2.exon1 | 30026184 | 30026330 |
| chr_2 | g8359 | g8359.t2 | exon | g8359.t2.exon2 | 30026588 | 30026675 |
| chr_2 | g8359 | g8359.t2 | exon | g8359.t2.exon3 | 30026745 | 30026943 |
| chr_2 | g8359 | g8359.t2 | exon | g8359.t2.exon4 | 30027002 | 30027726 |
| chr_2 | g8359 | g8359.t2 | cds | g8359.t2.CDS1 | 30027078 | 30027726 |
| chr_2 | g8359 | g8359.t2 | exon | g8359.t2.exon5 | 30027783 | 30027967 |
| chr_2 | g8359 | g8359.t2 | cds | g8359.t2.CDS2 | 30027783 | 30027967 |
| chr_2 | g8359 | g8359.t2 | TTS | g8359.t2 | 30028080 | 30028080 |
| chr_2 | g8359 | g8359.t2 | TSS | g8359.t2 | NA | NA |
Sequences
>g8359.t2 Gene=g8359 Length=1344
CCGTGTAAACGATGTACTGCAATTCTATCTTGGTATTAAAATCATATATTTTTATTCTCT
GATTTATCAAAACCAATACTTTTTATTTTATAAAATTATTCACGAGAATTACATTCTAAT
TTAAAAGTGCTACAAATTTTGACAAAAATTTTTCAAGATTTTGTTTCATCGAAACTACTT
AAAATAACTCATTTTAATGATGTTGGACCCAAAGTTTAAAAATCTTCCATTCTTGATGAG
AAAGATGTATATGAAACTGATGATCAATTATCCAGTGAAACTGATTATTACGAAGAGGAA
ACTGAGAATGAATCAATTGATCGCTTAAAATTAAATGTGAATGATGCTTTTTCAAAATTC
AAGGGAAAATATTTAATTGGAAATGTTGATTTCTCTGATAGCATAAGCAAGAGAAATTTT
GGCTATAATGCTGTCTCTGGAATCTATGAGATTGTTGGTGAAGGTGAGAAAGAAACACCC
GTTCAAAAACTTCAACGATTGCAATTTGAGATGAATGAATTGATGGAGGAAGTGACATCT
CTTGAGAATGATAAGAGCATTTCAAAAGAAGAGCATGAAGCATACTTTAAAATGTCGAAA
GTTGTGCAAAATTCTAAAAAGATTCTTGATAGCTTACACATTGAAGAAGCTTTAGGTGGC
CAAAACGGTGGTCAACCAGCTGAGAAAGCTGTTAAGAATCTAATTACTCAAGTTGATAGC
TATAAAAAAGGTGCACCAGAGATGTCTGCTGAATTAATTAAATTGAAAACGCAGAGTGAT
ATCACTTTTTCCACTCGCATTGCTGAGATGGAACATAAGTTACACAAAATTGAACAAACT
GTTGGTATGAAACCAGACAAGTTGTCTCGAATCAATAGTTCTTTAGATACTAAGAATCTC
CTTGAAGCTGTTCAACAATTATCAACGAGATCCGCTTTGATTCAACCGAGTCAATTGGAT
ATTATCGAACAGAGACTTACAATTTTATCATCGAAAATGGACCAATTTAAAGATAAAGCA
ATTGCTGCCGGAACTGACCGTGAACGTGAACAGAAAATTACTGAATTATATGATTTGGCA
AAATCAACTGAACCAATAACGAAAATACTTCCTGATATGCTCGAACGAATGAAAACTTTG
GAAGCACTCCATTCATATGCTGCAAACTTTAGTAAATTATTTGCTGAGTTAGAAGCAACA
CAGAATATTATTTTGAAGGGAATCGCTGGTAACAAGGAACTTTTGCAAGGTGTTCAAAAA
GCCTTTGTTGAAAATGATGAAAATGCTAAGAAAGAACTAAAAAAATTAGAAGAGCGTGTC
ATCGCTATAACAAATAAGAAGTAA
>g8359.t2 Gene=g8359 Length=277
MNELMEEVTSLENDKSISKEEHEAYFKMSKVVQNSKKILDSLHIEEALGGQNGGQPAEKA
VKNLITQVDSYKKGAPEMSAELIKLKTQSDITFSTRIAEMEHKLHKIEQTVGMKPDKLSR
INSSLDTKNLLEAVQQLSTRSALIQPSQLDIIEQRLTILSSKMDQFKDKAIAAGTDRERE
QKITELYDLAKSTEPITKILPDMLERMKTLEALHSYAANFSKLFAELEATQNIILKGIAG
NKELLQGVQKAFVENDENAKKELKKLEERVIAITNKK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 4 | g8359.t2 | Coils | Coil | Coil | 1 | 21 | - |
| 3 | g8359.t2 | Coils | Coil | Coil | 149 | 169 | - |
| 5 | g8359.t2 | Coils | Coil | Coil | 249 | 276 | - |
| 2 | g8359.t2 | PANTHER | PTHR15346 | DYNACTIN SUBUNIT | 2 | 274 | 2.5E-37 |
| 1 | g8359.t2 | Pfam | PF04912 | Dynamitin | 1 | 271 | 1.2E-55 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005869 | dynactin complex | CC |
| GO:0007017 | microtubule-based process | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
