General information for g836.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as MAP kinase-interacting serine/threonine-protein kinase 1.
Parent gene
- The parent gene g836 is located on chr_3: 6388313..6392170 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g836 | g836.t1 | TSS | g836.t1 | 6378136 | 6378136 |
| chr_3 | g836 | g836.t1 | isoform | g836.t1 | 6379073 | 6388550 |
| chr_3 | g836 | g836.t1 | exon | g836.t1.exon1 | 6379073 | 6379121 |
| chr_3 | g836 | g836.t1 | cds | g836.t1.CDS1 | 6379073 | 6379121 |
| chr_3 | g836 | g836.t1 | exon | g836.t1.exon2 | 6379810 | 6380016 |
| chr_3 | g836 | g836.t1 | cds | g836.t1.CDS2 | 6379810 | 6380016 |
| chr_3 | g836 | g836.t1 | exon | g836.t1.exon3 | 6385831 | 6386342 |
| chr_3 | g836 | g836.t1 | cds | g836.t1.CDS3 | 6385831 | 6386342 |
| chr_3 | g836 | g836.t1 | exon | g836.t1.exon4 | 6387724 | 6387949 |
| chr_3 | g836 | g836.t1 | cds | g836.t1.CDS4 | 6387724 | 6387949 |
| chr_3 | g836 | g836.t1 | exon | g836.t1.exon5 | 6388014 | 6388215 |
| chr_3 | g836 | g836.t1 | cds | g836.t1.CDS5 | 6388014 | 6388215 |
| chr_3 | g836 | g836.t1 | exon | g836.t1.exon6 | 6388283 | 6388550 |
| chr_3 | g836 | g836.t1 | cds | g836.t1.CDS6 | 6388283 | 6388550 |
| chr_3 | g836 | g836.t1 | TTS | g836.t1 | 6389365 | 6389365 |
Sequences
>g836.t1 Gene=g836 Length=1464
ATGGTGGAAAAAATTTTAGAAGAAGAGAAATCATCAGATACTAATTCAGAGAAGAGCTCT
GAGCCTCGAACACCAGCAGAGGTTACTCGCTATAGCAGCGAAGAGATGAATTCCGGCAAT
GAGTCAAATGAAGTGCATTTATGGACCGAGCAGGAGCGACAAGCCGACTTTAATCGCCAT
AAGGAGGAATTGCTGAAACGCAAACGCCGCCGCAAAAAACGTACTAGCTCATCAATTCAA
TCAAGTTGCTTTCAAGAACTGTATAAATTGACGGGCGAAATATTGGGAGAAGGTGCCTAT
GCCTCAGTACAAACCTGTATAAATATATTCACCGATGTAGAGTGTGCCGTTAAAATTATT
GATAAGATACCAGGCCATGCACGAGCTCGAGTATTTCGTGAAGTAGAGACGTTCCATCAT
TGTCAGGGACATCCGAATATTCTCCAATTAATTGAGTTCTTTGAGGATAAGGAGAAATTC
TATTTAGTATTTGAAAAGATCAATGGTGGTCCGCTATTGACGCGTATACAAGAGAACATT
TGCTTCTCAGAGCATGATGCAGCACAAATAATTAAGGAAATAGCCTCAGGATTGAATTTT
TTGCATAAGAAGGGCATAGCTCATCGTGATTTGAAACCTGAAAATATTTTATGCGTTAAT
CCGGACACAATTTGTCCCATTAAAATATGTGATTTTGATCTCGGCTCGGGAATTAATTTT
ACGACTGACGTTTCTGAATCAGTGGCCACTCCGCAACTTCTGACACCGGTTGGTTCCGCT
GAATTCATGGCTCCCGAAGTTGTCGACTTGTTTGTTGGCGAGCGCGAGAGCAGCTACGAT
AAAAGATGTGATTTGTGGTCAATGGGTGTCATTGCATATATCTTACTGTCTGGCTATCCG
CCATTCTCCGGCAATTGTGGTCAAGAATGTGGCTGGAATCGCGGTGAAAATTGTCAGAAA
TGTCAAGAGTTACTTTTTGAGTCAATTCGTGAAGGACGTTTTAGTTTTCCTGAAACCGAA
TGGCGTGACGTCAGCGAGGAGGCAAAAGATTTGATTCGTAATTTATTGGTAAAGGAAGCA
TCAAAGCGACTATCAGCTGAGGCTGTCTTAATTCATCCCTGGATCAAAGTCGCTGATGAA
GATTGTGACAACGAAAAACGCTATCGTGCGCTTAAAACTCCAGGGATCATTCGAAGCAAT
CAATCTGCTCGTAAATTATCTCAATTCGCTGAGTCGGCAATGGCTGTGAACCGAGTAATT
TTACAACAATTTTCCATGCAACTAAATTATTTGAACAACAAGGAGCGATCCAACATTTAT
CAACCATCAGTAAAGACGCAACAGCATCAACAGCATCATCAACATCACAGCAGCTCCAAT
GACTCATTTTTAATGCCAGAGTTGCCACCACCACTAAATACAGTACGTCAGCTAAGCAGT
AGTGACGATGATGAATTGCAATAA
>g836.t1 Gene=g836 Length=487
MVEKILEEEKSSDTNSEKSSEPRTPAEVTRYSSEEMNSGNESNEVHLWTEQERQADFNRH
KEELLKRKRRRKKRTSSSIQSSCFQELYKLTGEILGEGAYASVQTCINIFTDVECAVKII
DKIPGHARARVFREVETFHHCQGHPNILQLIEFFEDKEKFYLVFEKINGGPLLTRIQENI
CFSEHDAAQIIKEIASGLNFLHKKGIAHRDLKPENILCVNPDTICPIKICDFDLGSGINF
TTDVSESVATPQLLTPVGSAEFMAPEVVDLFVGERESSYDKRCDLWSMGVIAYILLSGYP
PFSGNCGQECGWNRGENCQKCQELLFESIREGRFSFPETEWRDVSEEAKDLIRNLLVKEA
SKRLSAEAVLIHPWIKVADEDCDNEKRYRALKTPGIIRSNQSARKLSQFAESAMAVNRVI
LQQFSMQLNYLNNKERSNIYQPSVKTQQHQQHHQHHSSSNDSFLMPELPPPLNTVRQLSS
SDDDELQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g836.t1 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 75 | 166 | 2.4E-33 |
| 6 | g836.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 167 | 375 | 2.3E-58 |
| 11 | g836.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 46 | - |
| 13 | g836.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 17 | - |
| 12 | g836.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 18 | 45 | - |
| 10 | g836.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 443 | 462 | - |
| 2 | g836.t1 | PANTHER | PTHR24349 | SERINE/THREONINE-PROTEIN KINASE | 66 | 427 | 6.8E-133 |
| 3 | g836.t1 | PANTHER | PTHR24349:SF254 | MAP KINASE-INTERACTING SERINE/THREONINE-PROTEIN KINASE 2 | 66 | 427 | 6.8E-133 |
| 1 | g836.t1 | Pfam | PF00069 | Protein kinase domain | 92 | 375 | 8.0E-65 |
| 8 | g836.t1 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 95 | 118 | - |
| 7 | g836.t1 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 206 | 218 | - |
| 14 | g836.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 89 | 375 | 43.693 |
| 9 | g836.t1 | SMART | SM00220 | serkin_6 | 89 | 375 | 1.6E-91 |
| 4 | g836.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 92 | 376 | 4.18E-72 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
- This transcript has been annotated with K04372 (MAP kinase interacting serine/threonine kinase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
