Gene loci information

Transcript annotation

  • This transcript has been annotated as MAP kinase-interacting serine/threonine-protein kinase 1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g836 g836.t2 TSS g836.t2 6378136 6378136
chr_3 g836 g836.t2 isoform g836.t2 6379073 6388550
chr_3 g836 g836.t2 exon g836.t2.exon1 6379073 6379121
chr_3 g836 g836.t2 cds g836.t2.CDS1 6379073 6379121
chr_3 g836 g836.t2 exon g836.t2.exon2 6379810 6380016
chr_3 g836 g836.t2 cds g836.t2.CDS2 6379810 6380016
chr_3 g836 g836.t2 exon g836.t2.exon3 6385362 6385457
chr_3 g836 g836.t2 cds g836.t2.CDS3 6385362 6385457
chr_3 g836 g836.t2 exon g836.t2.exon4 6385831 6386342
chr_3 g836 g836.t2 cds g836.t2.CDS4 6385831 6386342
chr_3 g836 g836.t2 exon g836.t2.exon5 6387724 6387949
chr_3 g836 g836.t2 cds g836.t2.CDS5 6387724 6387949
chr_3 g836 g836.t2 exon g836.t2.exon6 6388014 6388215
chr_3 g836 g836.t2 cds g836.t2.CDS6 6388014 6388215
chr_3 g836 g836.t2 exon g836.t2.exon7 6388283 6388550
chr_3 g836 g836.t2 cds g836.t2.CDS7 6388283 6388550
chr_3 g836 g836.t2 TTS g836.t2 6389365 6389365

Sequences

>g836.t2 Gene=g836 Length=1560
ATGGTGGAAAAAATTTTAGAAGAAGAGAAATCATCAGATACTAATTCAGAGAAGAGCTCT
GAGCCTCGAACACCAGCAGAGGTTACTCGCTATAGCAGCGAAGAGATGAATTCCGGCAAT
GAGTCAAATGAAGTGCATTTATGGACCGAGCAGGAGCGACAAGCCGACTTTAATCGCCAT
AAGGAGGAATTGCTGAAACGCAAACGCCGCCGCAAAAAACGTACTAGCTCATCAATTCAA
TCAAGTTGCTTTCAAGTAAATTTTAATAAAGGAGCAGAAGTGATTGTGCGAAAGTCACAG
CCAATTCGACGAAGAGTACGACAACGAAGGCCATTGATAACAAATTTCTCTGAACTGTAT
AAATTGACGGGCGAAATATTGGGAGAAGGTGCCTATGCCTCAGTACAAACCTGTATAAAT
ATATTCACCGATGTAGAGTGTGCCGTTAAAATTATTGATAAGATACCAGGCCATGCACGA
GCTCGAGTATTTCGTGAAGTAGAGACGTTCCATCATTGTCAGGGACATCCGAATATTCTC
CAATTAATTGAGTTCTTTGAGGATAAGGAGAAATTCTATTTAGTATTTGAAAAGATCAAT
GGTGGTCCGCTATTGACGCGTATACAAGAGAACATTTGCTTCTCAGAGCATGATGCAGCA
CAAATAATTAAGGAAATAGCCTCAGGATTGAATTTTTTGCATAAGAAGGGCATAGCTCAT
CGTGATTTGAAACCTGAAAATATTTTATGCGTTAATCCGGACACAATTTGTCCCATTAAA
ATATGTGATTTTGATCTCGGCTCGGGAATTAATTTTACGACTGACGTTTCTGAATCAGTG
GCCACTCCGCAACTTCTGACACCGGTTGGTTCCGCTGAATTCATGGCTCCCGAAGTTGTC
GACTTGTTTGTTGGCGAGCGCGAGAGCAGCTACGATAAAAGATGTGATTTGTGGTCAATG
GGTGTCATTGCATATATCTTACTGTCTGGCTATCCGCCATTCTCCGGCAATTGTGGTCAA
GAATGTGGCTGGAATCGCGGTGAAAATTGTCAGAAATGTCAAGAGTTACTTTTTGAGTCA
ATTCGTGAAGGACGTTTTAGTTTTCCTGAAACCGAATGGCGTGACGTCAGCGAGGAGGCA
AAAGATTTGATTCGTAATTTATTGGTAAAGGAAGCATCAAAGCGACTATCAGCTGAGGCT
GTCTTAATTCATCCCTGGATCAAAGTCGCTGATGAAGATTGTGACAACGAAAAACGCTAT
CGTGCGCTTAAAACTCCAGGGATCATTCGAAGCAATCAATCTGCTCGTAAATTATCTCAA
TTCGCTGAGTCGGCAATGGCTGTGAACCGAGTAATTTTACAACAATTTTCCATGCAACTA
AATTATTTGAACAACAAGGAGCGATCCAACATTTATCAACCATCAGTAAAGACGCAACAG
CATCAACAGCATCATCAACATCACAGCAGCTCCAATGACTCATTTTTAATGCCAGAGTTG
CCACCACCACTAAATACAGTACGTCAGCTAAGCAGTAGTGACGATGATGAATTGCAATAA

>g836.t2 Gene=g836 Length=519
MVEKILEEEKSSDTNSEKSSEPRTPAEVTRYSSEEMNSGNESNEVHLWTEQERQADFNRH
KEELLKRKRRRKKRTSSSIQSSCFQVNFNKGAEVIVRKSQPIRRRVRQRRPLITNFSELY
KLTGEILGEGAYASVQTCINIFTDVECAVKIIDKIPGHARARVFREVETFHHCQGHPNIL
QLIEFFEDKEKFYLVFEKINGGPLLTRIQENICFSEHDAAQIIKEIASGLNFLHKKGIAH
RDLKPENILCVNPDTICPIKICDFDLGSGINFTTDVSESVATPQLLTPVGSAEFMAPEVV
DLFVGERESSYDKRCDLWSMGVIAYILLSGYPPFSGNCGQECGWNRGENCQKCQELLFES
IREGRFSFPETEWRDVSEEAKDLIRNLLVKEASKRLSAEAVLIHPWIKVADEDCDNEKRY
RALKTPGIIRSNQSARKLSQFAESAMAVNRVILQQFSMQLNYLNNKERSNIYQPSVKTQQ
HQQHHQHHSSSNDSFLMPELPPPLNTVRQLSSSDDDELQ

Protein features from InterProScan

Transcript Database ID Name Start End E.value
5 g836.t2 Gene3D G3DSA:3.30.200.20 Phosphorylase Kinase; domain 1 110 198 4.1E-33
6 g836.t2 Gene3D G3DSA:1.10.510.10 Transferase(Phosphotransferase) domain 1 199 407 2.7E-58
11 g836.t2 MobiDBLite mobidb-lite consensus disorder prediction 1 46 -
14 g836.t2 MobiDBLite mobidb-lite consensus disorder prediction 1 17 -
12 g836.t2 MobiDBLite mobidb-lite consensus disorder prediction 18 45 -
13 g836.t2 MobiDBLite mobidb-lite consensus disorder prediction 59 78 -
10 g836.t2 MobiDBLite mobidb-lite consensus disorder prediction 475 494 -
2 g836.t2 PANTHER PTHR24349 SERINE/THREONINE-PROTEIN KINASE 103 459 4.8E-131
3 g836.t2 PANTHER PTHR24349:SF254 MAP KINASE-INTERACTING SERINE/THREONINE-PROTEIN KINASE 2 103 459 4.8E-131
1 g836.t2 Pfam PF00069 Protein kinase domain 124 407 9.6E-65
8 g836.t2 ProSitePatterns PS00107 Protein kinases ATP-binding region signature. 127 150 -
7 g836.t2 ProSitePatterns PS00108 Serine/Threonine protein kinases active-site signature. 238 250 -
15 g836.t2 ProSiteProfiles PS50011 Protein kinase domain profile. 121 407 43.693
9 g836.t2 SMART SM00220 serkin_6 121 407 1.6E-91
4 g836.t2 SUPERFAMILY SSF56112 Protein kinase-like (PK-like) 124 408 4.05E-72

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005524 ATP binding MF
GO:0004672 protein kinase activity MF
GO:0006468 protein phosphorylation BP

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values