General information for g836.t20
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative MAP kinase-interacting serine/threonine-protein kinase 1.
Parent gene
- The parent gene g836 is located on chr_3: 6388313..6392170 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g836 | g836.t20 | TSS | g836.t20 | 6385279 | 6385279 |
| chr_3 | g836 | g836.t20 | isoform | g836.t20 | 6385829 | 6389372 |
| chr_3 | g836 | g836.t20 | exon | g836.t20.exon1 | 6385829 | 6386342 |
| chr_3 | g836 | g836.t20 | exon | g836.t20.exon2 | 6387724 | 6387949 |
| chr_3 | g836 | g836.t20 | cds | g836.t20.CDS1 | 6387742 | 6387949 |
| chr_3 | g836 | g836.t20 | exon | g836.t20.exon3 | 6388014 | 6388215 |
| chr_3 | g836 | g836.t20 | cds | g836.t20.CDS2 | 6388014 | 6388215 |
| chr_3 | g836 | g836.t20 | exon | g836.t20.exon4 | 6388283 | 6389372 |
| chr_3 | g836 | g836.t20 | cds | g836.t20.CDS3 | 6388283 | 6388550 |
| chr_3 | g836 | g836.t20 | TTS | g836.t20 | 6389365 | 6389365 |
Sequences
>g836.t20 Gene=g836 Length=2032
AGAACTGTATAAATTGACGGGCGAAATATTGGGAGAAGGTGCCTATGCCTCAGTACAAAC
CTGTATAAATATATTCACCGATGTAGAGTGTGCCGTTAAAATTATTGATAAGATACCAGG
CCATGCACGAGCTCGAGTATTTCGTGAAGTAGAGACGTTCCATCATTGTCAGGGACATCC
GAATATTCTCCAATTAATTGAGTTCTTTGAGGATAAGGAGAAATTCTATTTAGTATTTGA
AAAGATCAATGGTGGTCCGCTATTGACGCGTATACAAGAGAACATTTGCTTCTCAGAGCA
TGATGCAGCACAAATAATTAAGGAAATAGCCTCAGGATTGAATTTTTTGCATAAGAAGGG
CATAGCTCATCGTGATTTGAAACCTGAAAATATTTTATGCGTTAATCCGGACACAATTTG
TCCCATTAAAATATGTGATTTTGATCTCGGCTCGGGAATTAATTTTACGACTGACGTTTC
TGAATCAGTGGCCACTCCGCAACTTCTGACACCGGTTGGTTCCGCTGAATTCATGGCTCC
CGAAGTTGTCGACTTGTTTGTTGGCGAGCGCGAGAGCAGCTACGATAAAAGATGTGATTT
GTGGTCAATGGGTGTCATTGCATATATCTTACTGTCTGGCTATCCGCCATTCTCCGGCAA
TTGTGGTCAAGAATGTGGCTGGAATCGCGGTGAAAATTGTCAGAAATGTCAAGAGTTACT
TTTTGAGTCAATTCGTGAAGGACGTTTTAGTTTTCCTGAAACCGAATGGCGTGACGTCAG
CGAGGAGGCAAAAGATTTGATTCGTAATTTATTGGTAAAGGAAGCATCAAAGCGACTATC
AGCTGAGGCTGTCTTAATTCATCCCTGGATCAAAGTCGCTGATGAAGATTGTGACAACGA
AAAACGCTATCGTGCGCTTAAAACTCCAGGGATCATTCGAAGCAATCAATCTGCTCGTAA
ATTATCTCAATTCGCTGAGTCGGCAATGGCTGTGAACCGAGTAATTTTACAACAATTTTC
CATGCAACTAAATTATTTGAACAACAAGGAGCGATCCAACATTTATCAACCATCAGTAAA
GACGCAACAGCATCAACAGCATCATCAACATCACAGCAGCTCCAATGACTCATTTTTAAT
GCCAGAGTTGCCACCACCACTAAATACAGTACGTCAGCTAAGCAGTAGTGACGATGATGA
ATTGCAATAAAATGCAGTGAATCATCCTCGTGTGGTTCCAATCCGATGACACCTTCAGGC
AATCATCGGATGAAGACATTCAGACCATTTTAGACAATGTGCTTTTGACCAACAACAGTG
CTGGCATGAGTAACATCATCAACAAATGTGATGACGCTGTCAACAGTCCTTTGCAGTCAC
GTCGTCGTCAAACACGACCACTTTTTATTGTATGTCCAACACCAAGTGGCTGATAAACCT
GTATGGAGAAACTACCAAGTACTTTCTTCTTCTTCTATTCTTCCTACATAACCATTCATC
CATCTTATATCTTATGGAAAATTATTGATAAAACAGAACTCTTTCTAATCTAATCTGCAC
CTATCTAATGAATGAATATTATGTAGTTCCATCTAATGAACTTACTTTAATAATTTATTC
CCAAAACAAAAAGAGAGTTACAAATTTTTAAATATATACTGCATAGTCTCGAGACCTCAG
TGCAAAAGAATTTTAATAATGATAACAATTGAATGATGAGAAACATCTTAGAATATTTCC
ATTAGCTTTGATTGAATCACAAAAAAAGTTCGATAAAACACAAATCATCTTAAAATTATT
TTTATTCTCTTCTTAATTTTTTATTAAACTATTTCTCCTGAACCAATTCAAATTTTGTAA
GCGTAGAAAATGATAAAATTACTTAAAAAACATGTTCTCAAAAAATATGAAAGAAAAATC
ATGAAAATCTTTCAGAAAAAAACACAAAAATAATTTTTCTTTTATAAGTTTTTTTATTTT
TTTTTGTCAAACATTTGCAATAAAAATCTAATTTTTTATATCTGAATGAAAA
>g836.t20 Gene=g836 Length=225
MAPEVVDLFVGERESSYDKRCDLWSMGVIAYILLSGYPPFSGNCGQECGWNRGENCQKCQ
ELLFESIREGRFSFPETEWRDVSEEAKDLIRNLLVKEASKRLSAEAVLIHPWIKVADEDC
DNEKRYRALKTPGIIRSNQSARKLSQFAESAMAVNRVILQQFSMQLNYLNNKERSNIYQP
SVKTQQHQQHHQHHSSSNDSFLMPELPPPLNTVRQLSSSDDDELQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g836.t20 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 1 | 119 | 1.3E-33 |
| 7 | g836.t20 | Gene3D | G3DSA:1.10.238.10 | - | 120 | 171 | 1.3E-33 |
| 5 | g836.t20 | MobiDBLite | mobidb-lite | consensus disorder prediction | 181 | 200 | - |
| 2 | g836.t20 | PANTHER | PTHR24349 | SERINE/THREONINE-PROTEIN KINASE | 1 | 196 | 4.4E-45 |
| 3 | g836.t20 | PANTHER | PTHR24349:SF114 | MAP KINASE-INTERACTING SERINE/THREONINE-PROTEIN KINASE 1 | 1 | 196 | 4.4E-45 |
| 1 | g836.t20 | Pfam | PF00069 | Protein kinase domain | 1 | 113 | 1.3E-18 |
| 8 | g836.t20 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 1 | 113 | 18.026 |
| 4 | g836.t20 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 1 | 114 | 3.62E-22 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
