Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative MAP kinase-interacting serine/threonine-protein kinase 1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g836 g836.t26 TSS g836.t26 6387723 6387723
chr_3 g836 g836.t26 isoform g836.t26 6387793 6392170
chr_3 g836 g836.t26 exon g836.t26.exon1 6387793 6387949
chr_3 g836 g836.t26 cds g836.t26.CDS1 6387817 6387949
chr_3 g836 g836.t26 exon g836.t26.exon2 6388014 6388215
chr_3 g836 g836.t26 cds g836.t26.CDS2 6388014 6388215
chr_3 g836 g836.t26 exon g836.t26.exon3 6388283 6389392
chr_3 g836 g836.t26 cds g836.t26.CDS3 6388283 6388550
chr_3 g836 g836.t26 exon g836.t26.exon4 6392107 6392170
chr_3 g836 g836.t26 TTS g836.t26 6392157 6392157

Sequences

>g836.t26 Gene=g836 Length=1533
GATAAAAGATGTGATTTGTGGTCAATGGGTGTCATTGCATATATCTTACTGTCTGGCTAT
CCGCCATTCTCCGGCAATTGTGGTCAAGAATGTGGCTGGAATCGCGGTGAAAATTGTCAG
AAATGTCAAGAGTTACTTTTTGAGTCAATTCGTGAAGGACGTTTTAGTTTTCCTGAAACC
GAATGGCGTGACGTCAGCGAGGAGGCAAAAGATTTGATTCGTAATTTATTGGTAAAGGAA
GCATCAAAGCGACTATCAGCTGAGGCTGTCTTAATTCATCCCTGGATCAAAGTCGCTGAT
GAAGATTGTGACAACGAAAAACGCTATCGTGCGCTTAAAACTCCAGGGATCATTCGAAGC
AATCAATCTGCTCGTAAATTATCTCAATTCGCTGAGTCGGCAATGGCTGTGAACCGAGTA
ATTTTACAACAATTTTCCATGCAACTAAATTATTTGAACAACAAGGAGCGATCCAACATT
TATCAACCATCAGTAAAGACGCAACAGCATCAACAGCATCATCAACATCACAGCAGCTCC
AATGACTCATTTTTAATGCCAGAGTTGCCACCACCACTAAATACAGTACGTCAGCTAAGC
AGTAGTGACGATGATGAATTGCAATAAAATGCAGTGAATCATCCTCGTGTGGTTCCAATC
CGATGACACCTTCAGGCAATCATCGGATGAAGACATTCAGACCATTTTAGACAATGTGCT
TTTGACCAACAACAGTGCTGGCATGAGTAACATCATCAACAAATGTGATGACGCTGTCAA
CAGTCCTTTGCAGTCACGTCGTCGTCAAACACGACCACTTTTTATTGTATGTCCAACACC
AAGTGGCTGATAAACCTGTATGGAGAAACTACCAAGTACTTTCTTCTTCTTCTATTCTTC
CTACATAACCATTCATCCATCTTATATCTTATGGAAAATTATTGATAAAACAGAACTCTT
TCTAATCTAATCTGCACCTATCTAATGAATGAATATTATGTAGTTCCATCTAATGAACTT
ACTTTAATAATTTATTCCCAAAACAAAAAGAGAGTTACAAATTTTTAAATATATACTGCA
TAGTCTCGAGACCTCAGTGCAAAAGAATTTTAATAATGATAACAATTGAATGATGAGAAA
CATCTTAGAATATTTCCATTAGCTTTGATTGAATCACAAAAAAAGTTCGATAAAACACAA
ATCATCTTAAAATTATTTTTATTCTCTTCTTAATTTTTTATTAAACTATTTCTCCTGAAC
CAATTCAAATTTTGTAAGCGTAGAAAATGATAAAATTACTTAAAAAACATGTTCTCAAAA
AATATGAAAGAAAAATCATGAAAATCTTTCAGAAAAAAACACAAAAATAATTTTTCTTTT
ATAAGTTTTTTTATTTTTTTTTGTCAAACATTTGCAATAAAAATCTAATTTTTTATATCT
GAATGAAAATAGATTAGCATTATTATTATGAAAAACTGTGAGTAAAAAAGTACATTGAAT
TTATCATGAGCGTAAAAAAGTAAAAAAAAAAAA

>g836.t26 Gene=g836 Length=200
MGVIAYILLSGYPPFSGNCGQECGWNRGENCQKCQELLFESIREGRFSFPETEWRDVSEE
AKDLIRNLLVKEASKRLSAEAVLIHPWIKVADEDCDNEKRYRALKTPGIIRSNQSARKLS
QFAESAMAVNRVILQQFSMQLNYLNNKERSNIYQPSVKTQQHQQHHQHHSSSNDSFLMPE
LPPPLNTVRQLSSSDDDELQ

Protein features from InterProScan

Transcript Database ID Name Start End E.value
6 g836.t26 Gene3D G3DSA:1.10.510.10 Transferase(Phosphotransferase) domain 1 1 94 8.6E-25
7 g836.t26 Gene3D G3DSA:1.10.238.10 - 95 147 8.6E-25
5 g836.t26 MobiDBLite mobidb-lite consensus disorder prediction 156 175 -
2 g836.t26 PANTHER PTHR24349 SERINE/THREONINE-PROTEIN KINASE 1 171 2.4E-32
3 g836.t26 PANTHER PTHR24349:SF114 MAP KINASE-INTERACTING SERINE/THREONINE-PROTEIN KINASE 1 1 171 2.4E-32
1 g836.t26 Pfam PF00069 Protein kinase domain 1 88 2.2E-10
8 g836.t26 ProSiteProfiles PS50011 Protein kinase domain profile. 1 88 11.694
4 g836.t26 SUPERFAMILY SSF56112 Protein kinase-like (PK-like) 1 89 2.41E-14

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005524 ATP binding MF
GO:0004672 protein kinase activity MF
GO:0006468 protein phosphorylation BP

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values