Gene loci information

Transcript annotation

  • This transcript has been annotated as MAP kinase-interacting serine/threonine-protein kinase 1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g836 g836.t9 TSS g836.t9 6378136 6378136
chr_3 g836 g836.t9 isoform g836.t9 6378139 6387949
chr_3 g836 g836.t9 exon g836.t9.exon1 6378139 6379121
chr_3 g836 g836.t9 exon g836.t9.exon2 6379815 6380016
chr_3 g836 g836.t9 cds g836.t9.CDS1 6379866 6380016
chr_3 g836 g836.t9 exon g836.t9.exon3 6385831 6386342
chr_3 g836 g836.t9 cds g836.t9.CDS2 6385831 6386342
chr_3 g836 g836.t9 exon g836.t9.exon4 6387724 6387949
chr_3 g836 g836.t9 cds g836.t9.CDS3 6387724 6387948
chr_3 g836 g836.t9 TTS g836.t9 NA NA

Sequences

>g836.t9 Gene=g836 Length=1923
TTTGTGAGAGAACATCGGAAAGGACAGACTGAGAAGTGAAATGGAGTGAATTGTTAAGTG
AAAAAAGAAAAACTCAAAGAAGTTTTTTTCTTTTTGATGAGAAAACATCGGCCTTTTGTG
TGTGTCTCGCTATAATAGCGTTCTAACAATCATCGGTTTTTAGTCCACACCATATTCATC
CCACTGTAAAGGAAAAGTTAAAAGTAATCGATTAAATCAGGCAGGAGAAAATAGATCTGG
AAGTTTTTTATTGATTTACAAAAAAAATCGGCCGAGAGAAGAAATACAAGGCAGTGAGAA
AGAGAAAGAAGAGCATCGAGAAAAAAAATCCAGTTTGGCTGAAAGTTTTTTTTCTCATTG
AATGTTTTTTATTTCTAAAAAGCTATAAAATTGCAAGCAAAGTGGCAAAAATTTCACTCG
GAAAGGAAAATTAAAAATTATCTTTTTTGCCCTGCAACAACAACCACAACACTCAACCCA
CAACAACAACAAATCATCTACGAATCGTTATATGCTAACTTTTTGTTAATTTAATTTTAA
CACAAATCAACCAAAAGAAAGAAAGATAGAAGGATCAACAGAAGTTGAATTGAATGAATG
CATAAAAAATCTCTTTAAGGTCACATTTTAAGAGAAGCCAGAAAATTAATTATAATTAAT
TAAATAAGCTTAAGAGAAAAGATAGCTTGAGTTAGTTTTGAAAATTTAAAAAAATTACAT
CTAATGCGAGAAGCAAGTAAAGTGAATATTCATGTGAGAGCTTAAAGTTAATCGAAAGAA
AAAAAATAATTTTTAATAAGTGAAATATTCTTCGGGAAAAGCAAGAATCTACGCAAAAGA
ATTGTTAAATTTATTCACCAAATTTGAAATAATAAAAAATAAGAAGTATTTTTTTCGTGT
TCGAGAGAGAGAGAGAAAAAAAAGATACACAAAAATGGTGGAAAAAATTTTAGAAGAAGA
GAAATCATCAGATACTAATTCAGAGCTCTGAGCCTCGAACACCAGCAGAGGTTACTCGCT
ATAGCAGCGAAGAGATGAATTCCGGCAATGAGTCAAATGAAGTGCATTTATGGACCGAGC
AGGAGCGACAAGCCGACTTTAATCGCCATAAGGAGGAATTGCTGAAACGCAAACGCCGCC
GCAAAAAACGTACTAGCTCATCAATTCAATCAAGTTGCTTTCAAGAACTGTATAAATTGA
CGGGCGAAATATTGGGAGAAGGTGCCTATGCCTCAGTACAAACCTGTATAAATATATTCA
CCGATGTAGAGTGTGCCGTTAAAATTATTGATAAGATACCAGGCCATGCACGAGCTCGAG
TATTTCGTGAAGTAGAGACGTTCCATCATTGTCAGGGACATCCGAATATTCTCCAATTAA
TTGAGTTCTTTGAGGATAAGGAGAAATTCTATTTAGTATTTGAAAAGATCAATGGTGGTC
CGCTATTGACGCGTATACAAGAGAACATTTGCTTCTCAGAGCATGATGCAGCACAAATAA
TTAAGGAAATAGCCTCAGGATTGAATTTTTTGCATAAGAAGGGCATAGCTCATCGTGATT
TGAAACCTGAAAATATTTTATGCGTTAATCCGGACACAATTTGTCCCATTAAAATATGTG
ATTTTGATCTCGGCTCGGGAATTAATTTTACGACTGACGTTTCTGAATCAGTGGCCACTC
CGCAACTTCTGACACCGGTTGGTTCCGCTGAATTCATGGCTCCCGAAGTTGTCGACTTGT
TTGTTGGCGAGCGCGAGAGCAGCTACGATAAAAGATGTGATTTGTGGTCAATGGGTGTCA
TTGCATATATCTTACTGTCTGGCTATCCGCCATTCTCCGGCAATTGTGGTCAAGAATGTG
GCTGGAATCGCGGTGAAAATTGTCAGAAATGTCAAGAGTTACTTTTTGAGTCAATTCGTG
AAG

>g836.t9 Gene=g836 Length=296
MNSGNESNEVHLWTEQERQADFNRHKEELLKRKRRRKKRTSSSIQSSCFQELYKLTGEIL
GEGAYASVQTCINIFTDVECAVKIIDKIPGHARARVFREVETFHHCQGHPNILQLIEFFE
DKEKFYLVFEKINGGPLLTRIQENICFSEHDAAQIIKEIASGLNFLHKKGIAHRDLKPEN
ILCVNPDTICPIKICDFDLGSGINFTTDVSESVATPQLLTPVGSAEFMAPEVVDLFVGER
ESSYDKRCDLWSMGVIAYILLSGYPPFSGNCGQECGWNRGENCQKCQELLFESIRE

Protein features from InterProScan

Transcript Database ID Name Start End E.value
8 g836.t9 Gene3D G3DSA:3.30.200.20 Phosphorylase Kinase; domain 1 40 131 9.9E-34
9 g836.t9 Gene3D G3DSA:1.10.510.10 Transferase(Phosphotransferase) domain 1 132 296 5.5E-42
2 g836.t9 PANTHER PTHR24349 SERINE/THREONINE-PROTEIN KINASE 31 296 5.9E-108
3 g836.t9 PANTHER PTHR24349:SF254 MAP KINASE-INTERACTING SERINE/THREONINE-PROTEIN KINASE 2 31 296 5.9E-108
1 g836.t9 Pfam PF00069 Protein kinase domain 57 288 5.0E-55
7 g836.t9 ProSitePatterns PS00107 Protein kinases ATP-binding region signature. 60 83 -
6 g836.t9 ProSitePatterns PS00108 Serine/Threonine protein kinases active-site signature. 171 183 -
10 g836.t9 ProSiteProfiles PS50011 Protein kinase domain profile. 54 296 38.435
5 g836.t9 SMART SM00220 serkin_6 54 291 7.4E-61
4 g836.t9 SUPERFAMILY SSF56112 Protein kinase-like (PK-like) 57 273 8.09E-61

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005524 ATP binding MF
GO:0004672 protein kinase activity MF
GO:0006468 protein phosphorylation BP

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values