General information for g8453.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Chymotrypsin-1.
Parent gene
- The parent gene g8453 is located on chr_2: 30789279..30789950 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8453 | g8453.t1 | TTS | g8453.t1 | 30788597 | 30788597 |
| chr_2 | g8453 | g8453.t1 | isoform | g8453.t1 | 30788615 | 30789950 |
| chr_2 | g8453 | g8453.t1 | exon | g8453.t1.exon1 | 30788615 | 30788672 |
| chr_2 | g8453 | g8453.t1 | cds | g8453.t1.CDS1 | 30788615 | 30788672 |
| chr_2 | g8453 | g8453.t1 | exon | g8453.t1.exon2 | 30788736 | 30788819 |
| chr_2 | g8453 | g8453.t1 | cds | g8453.t1.CDS2 | 30788736 | 30788819 |
| chr_2 | g8453 | g8453.t1 | exon | g8453.t1.exon3 | 30789236 | 30789408 |
| chr_2 | g8453 | g8453.t1 | cds | g8453.t1.CDS3 | 30789236 | 30789408 |
| chr_2 | g8453 | g8453.t1 | exon | g8453.t1.exon4 | 30789466 | 30789842 |
| chr_2 | g8453 | g8453.t1 | cds | g8453.t1.CDS4 | 30789466 | 30789842 |
| chr_2 | g8453 | g8453.t1 | exon | g8453.t1.exon5 | 30789896 | 30789950 |
| chr_2 | g8453 | g8453.t1 | cds | g8453.t1.CDS5 | 30789896 | 30789950 |
| chr_2 | g8453 | g8453.t1 | TSS | g8453.t1 | 30789966 | 30789966 |
Sequences
>g8453.t1 Gene=g8453 Length=747
ATGAAGAATTCAGTTGTGATTTTTTTACTCTTCAATTTTATTTTTATTTCAAATGCTTTT
TTCGATTCAAAAATCGTTGGAGGATCAACAGTGCAACTTGGTCAAATTCCCTCACAAATT
TCACTTAGAACTCAAGCAAATGTTCATTTTTGTGGAGGTGCATTGATTTCAGGTCGTTGG
GTGATCACAAGTGGTCAATGCACATCAGGTCGAGCTCATAATTCAATAAATGCTGTTGCT
GGAACTGTGACATTAAATGTAGGTGGTATAACACAAAGGAGTGTCAGAATTGTTCAACAT
CCACAGTTTCATATTATAAGTTTGGCCAATGATGTGTCACTGATTGAAACTGAAAATTCT
TTTACTATGACGCAACACATTCAAACGATTAATCTCGGAGTTGCTAATTTAGGTGAAAAT
ATTGCAGTTCAATTAAGCGGCTTTGGAGCAACACAAAGTTCTGGTCCGTTATCAAATAAT
CTACAAAGAGTCAATGTTGTTACAATCACAAATGCCGCATGCATTTCACAACTTGGCAAT
CAAAACCTTCAAGTGAATGACGCTAAATTGTGTACTTTTTTAAGTGGTCGTGGAATGTGC
ACTGGTGATCAAGGAGGACCAGTGATTGACATGAATAACCAACTTGTTGCTATCAAATCT
TGGAATATCCAATGCTTTCAAAATTTTCCAGACGGACATGAGAGAATTTTTGTACACAGA
AATTGGATTTTAAGTGTAATTTCCTAA
>g8453.t1 Gene=g8453 Length=248
MKNSVVIFLLFNFIFISNAFFDSKIVGGSTVQLGQIPSQISLRTQANVHFCGGALISGRW
VITSGQCTSGRAHNSINAVAGTVTLNVGGITQRSVRIVQHPQFHIISLANDVSLIETENS
FTMTQHIQTINLGVANLGENIAVQLSGFGATQSSGPLSNNLQRVNVVTITNAACISQLGN
QNLQVNDAKLCTFLSGRGMCTGDQGGPVIDMNNQLVAIKSWNIQCFQNFPDGHERIFVHR
NWILSVIS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 16 | g8453.t1 | CDD | cd00190 | Tryp_SPc | 25 | 243 | 3.57825E-51 |
| 9 | g8453.t1 | Gene3D | G3DSA:2.40.10.10 | - | 25 | 234 | 1.1E-42 |
| 10 | g8453.t1 | Gene3D | G3DSA:2.40.10.10 | - | 37 | 126 | 1.1E-42 |
| 2 | g8453.t1 | PANTHER | PTHR24276 | POLYSERASE-RELATED | 6 | 243 | 1.3E-41 |
| 3 | g8453.t1 | PANTHER | PTHR24276:SF78 | AT20289P-RELATED | 6 | 243 | 1.3E-41 |
| 6 | g8453.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 52 | 67 | 1.8E-9 |
| 5 | g8453.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 107 | 121 | 1.8E-9 |
| 4 | g8453.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 197 | 209 | 1.8E-9 |
| 1 | g8453.t1 | Pfam | PF00089 | Trypsin | 25 | 243 | 9.0E-37 |
| 12 | g8453.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 21 | - |
| 13 | g8453.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 4 | - |
| 14 | g8453.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 5 | 16 | - |
| 15 | g8453.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 17 | 21 | - |
| 11 | g8453.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 22 | 248 | - |
| 18 | g8453.t1 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 25 | 248 | 22.571 |
| 17 | g8453.t1 | SMART | SM00020 | trypsin_2 | 24 | 243 | 3.8E-43 |
| 7 | g8453.t1 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 18 | 247 | 6.49E-53 |
| 8 | g8453.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 19 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed