General information for g8463.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Serine/threonine-protein kinase PAK mbt.
Parent gene
- The parent gene g8463 is located on chr_2: 30843435..30843991 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8463 | g8463.t3 | isoform | g8463.t3 | 30843435 | 30843991 |
| chr_2 | g8463 | g8463.t3 | exon | g8463.t3.exon1 | 30843435 | 30843991 |
| chr_2 | g8463 | g8463.t3 | cds | g8463.t3.CDS1 | 30843437 | 30843991 |
| chr_2 | g8463 | g8463.t3 | TSS | g8463.t3 | 30844256 | 30844256 |
| chr_2 | g8463 | g8463.t3 | TTS | g8463.t3 | NA | NA |
Sequences
>g8463.t3 Gene=g8463 Length=557
ATGTTTTCTAAAAAGAAAAAGAAACCGCAAATTTCACTTCCGTCCAATTTTGAACATCGT
GTACATACAGGATTTGACAAAAAGGAGGGAAAATATATTGGATTACCGCTACAATGGGCA
TCAATTGTGGGAAATAATCAAATTTTAAAAAGCAGCAATCGGCCGCTGCCATTAGTTGAT
CCATCTGCAATTACTCCCACTGAAATTCTAGATTTAAAGACAATTGTACGACCTCAAAGT
AGTTTAGCAACATCAATTGGTGACAGTTCTATGTCGTCTTCCTCACTCGTTGGTCCAGAA
TTACACAACAATTCTTATAATGGCTTTAATCTACCGAAAATTTCACATGTAGCACGCTCA
AATTCATTACGATGTTCAAGTCCGCCTCGTCTTTATCAACGTGAACCTCAAATTCCTAAT
TCAGTGCAAGAAGAAACAACACAACAACAGCAACCTCATCCTATGATACCTAATCATCAT
CAACAGCAGCAGCAACAACAACAATATCCTTTAGTCCATCAAATTGATCCAAATATCAGA
AAACAATTACAGCCTCA
>g8463.t3 Gene=g8463 Length=185
MFSKKKKKPQISLPSNFEHRVHTGFDKKEGKYIGLPLQWASIVGNNQILKSSNRPLPLVD
PSAITPTEILDLKTIVRPQSSLATSIGDSSMSSSSLVGPELHNNSYNGFNLPKISHVARS
NSLRCSSPPRLYQREPQIPNSVQEETTQQQQPHPMIPNHHQQQQQQQQYPLVHQIDPNIR
KQLQP
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g8463.t3 | CDD | cd01093 | CRIB_PAK_like | 9 | 54 | 2.0199E-16 |
| 6 | g8463.t3 | Gene3D | G3DSA:3.90.810.10 | - | 4 | 53 | 3.7E-16 |
| 5 | g8463.t3 | MobiDBLite | mobidb-lite | consensus disorder prediction | 120 | 185 | - |
| 2 | g8463.t3 | PANTHER | PTHR48015 | SERINE/THREONINE-PROTEIN KINASE TAO | 1 | 167 | 4.0E-30 |
| 3 | g8463.t3 | PANTHER | PTHR48015:SF4 | SERINE/THREONINE-PROTEIN KINASE PAK MBT | 1 | 167 | 4.0E-30 |
| 1 | g8463.t3 | Pfam | PF00786 | P21-Rho-binding domain | 10 | 48 | 2.3E-11 |
| 7 | g8463.t3 | ProSiteProfiles | PS50108 | CRIB domain profile. | 11 | 24 | 8.855 |
| 4 | g8463.t3 | SMART | SM00285 | PBD_5 | 11 | 46 | 1.4E-11 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004674 | protein serine/threonine kinase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
