General information for g8505.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Potassium channel subfamily K member 9.
Parent gene
- The parent gene g8505 is located on chr_2: 31063928..31067728 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8505 | g8505.t1 | TSS | g8505.t1 | 31062960 | 31062960 |
| chr_2 | g8505 | g8505.t1 | isoform | g8505.t1 | 31063613 | 31064557 |
| chr_2 | g8505 | g8505.t1 | exon | g8505.t1.exon1 | 31063613 | 31063691 |
| chr_2 | g8505 | g8505.t1 | cds | g8505.t1.CDS1 | 31063613 | 31063691 |
| chr_2 | g8505 | g8505.t1 | exon | g8505.t1.exon2 | 31063749 | 31063928 |
| chr_2 | g8505 | g8505.t1 | cds | g8505.t1.CDS2 | 31063749 | 31063928 |
| chr_2 | g8505 | g8505.t1 | exon | g8505.t1.exon3 | 31064064 | 31064301 |
| chr_2 | g8505 | g8505.t1 | cds | g8505.t1.CDS3 | 31064064 | 31064301 |
| chr_2 | g8505 | g8505.t1 | exon | g8505.t1.exon4 | 31064383 | 31064557 |
| chr_2 | g8505 | g8505.t1 | cds | g8505.t1.CDS4 | 31064383 | 31064557 |
| chr_2 | g8505 | g8505.t1 | TTS | g8505.t1 | NA | NA |
Sequences
>g8505.t1 Gene=g8505 Length=672
ATGAAAAAGCAAAATATTCGTACGCTTTCTTTAATCGTGTGCACTTTTACATATTTGCTA
ATTGGGGCTGCTGTTTTTGATGCACTTGAATCAGATACAGAAAGAAAGCGTTGGGAAGCA
TTACAAGCAATTGAAGATATCATTGTCCAAAAATATAATATCACATCGGATGATTTGAAA
ATCATTGAAACAGTAATTCTAAAAACAGAGCCACATAAGGCAGGACGACAGTGGAAGTTT
ACCGGAGCATTTTATTATGTGTTAACTGTGCTAACAACAATTGGCTACGGTCATTCAACA
CCATCAACAGTTTATGGAAAATTGTTTACAATGTGCTATGCAATGATAGGAATACCTTTG
GGTAAATTTTCATTATCGTCTTATAAAACTGTTCAGTCAATAAAATTCTTCATTTCTTCT
TCATTTCTTATTCATTCGATTGTTATAGGACTTGTTATGTTTCAGAGTATAGGTGAACGA
GTCAATAGACTGAGTAGTTTTGTTATCCAGTCGGTTAAGAAATCATTAAACTGCCGAAAA
ACACAAGCTACAGAAGTGGATTTAATTTGTGTCGTTACAACTCTTAGCAGCCTAACAATT
GCAGGCGGTGCTTGGGCATTCTCAAAATTTGGTATGAAAAGAAATCTTACATTTAATGTA
TTAAAATTATAA
>g8505.t1 Gene=g8505 Length=223
MKKQNIRTLSLIVCTFTYLLIGAAVFDALESDTERKRWEALQAIEDIIVQKYNITSDDLK
IIETVILKTEPHKAGRQWKFTGAFYYVLTVLTTIGYGHSTPSTVYGKLFTMCYAMIGIPL
GKFSLSSYKTVQSIKFFISSSFLIHSIVIGLVMFQSIGERVNRLSSFVIQSVKKSLNCRK
TQATEVDLICVVTTLSSLTIAGGAWAFSKFGMKRNLTFNVLKL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g8505.t1 | Gene3D | G3DSA:1.10.287.70 | - | 1 | 219 | 5.8E-35 |
| 3 | g8505.t1 | PANTHER | PTHR11003 | POTASSIUM CHANNEL, SUBFAMILY K | 1 | 127 | 3.8E-75 |
| 5 | g8505.t1 | PANTHER | PTHR11003:SF253 | IP11374P | 1 | 127 | 3.8E-75 |
| 2 | g8505.t1 | PANTHER | PTHR11003 | POTASSIUM CHANNEL, SUBFAMILY K | 142 | 210 | 3.8E-75 |
| 4 | g8505.t1 | PANTHER | PTHR11003:SF253 | IP11374P | 142 | 210 | 3.8E-75 |
| 7 | g8505.t1 | PRINTS | PR01095 | TASK K+ channel signature | 11 | 33 | 5.6E-19 |
| 6 | g8505.t1 | PRINTS | PR01095 | TASK K+ channel signature | 151 | 167 | 5.6E-19 |
| 1 | g8505.t1 | Pfam | PF07885 | Ion channel | 75 | 120 | 1.2E-15 |
| 20 | g8505.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 5 | - |
| 24 | g8505.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 6 | 29 | - |
| 15 | g8505.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 30 | 77 | - |
| 23 | g8505.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 78 | 98 | - |
| 18 | g8505.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 99 | 103 | - |
| 22 | g8505.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 104 | 125 | - |
| 16 | g8505.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 126 | 136 | - |
| 21 | g8505.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 137 | 157 | - |
| 19 | g8505.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 158 | 185 | - |
| 25 | g8505.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 186 | 207 | - |
| 17 | g8505.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 208 | 223 | - |
| 13 | g8505.t1 | SUPERFAMILY | SSF81324 | Voltage-gated potassium channels | 8 | 125 | 9.16E-21 |
| 9 | g8505.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 29 | - |
| 8 | g8505.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 80 | 97 | - |
| 12 | g8505.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 104 | 121 | - |
| 10 | g8505.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 136 | 158 | - |
| 11 | g8505.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 188 | 207 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0005267 | potassium channel activity | MF |
| GO:0071805 | potassium ion transmembrane transport | BP |
KEGG
Orthology
- This transcript has been annotated with K04919 (potassium channel subfamily K member 9)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed