General information for g8512.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Ubiquitin-protein ligase E3B.
Parent gene
- The parent gene g8512 is located on chr_2: 31078849..31080843 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8512 | g8512.t1 | TSS | g8512.t1 | 31074777 | 31074777 |
| chr_2 | g8512 | g8512.t1 | isoform | g8512.t1 | 31074925 | 31078849 |
| chr_2 | g8512 | g8512.t1 | exon | g8512.t1.exon1 | 31074925 | 31075094 |
| chr_2 | g8512 | g8512.t1 | cds | g8512.t1.CDS1 | 31074925 | 31075094 |
| chr_2 | g8512 | g8512.t1 | exon | g8512.t1.exon2 | 31075152 | 31076206 |
| chr_2 | g8512 | g8512.t1 | cds | g8512.t1.CDS2 | 31075152 | 31076206 |
| chr_2 | g8512 | g8512.t1 | exon | g8512.t1.exon3 | 31076267 | 31077085 |
| chr_2 | g8512 | g8512.t1 | cds | g8512.t1.CDS3 | 31076267 | 31077085 |
| chr_2 | g8512 | g8512.t1 | exon | g8512.t1.exon4 | 31077160 | 31077455 |
| chr_2 | g8512 | g8512.t1 | cds | g8512.t1.CDS4 | 31077160 | 31077455 |
| chr_2 | g8512 | g8512.t1 | exon | g8512.t1.exon5 | 31077558 | 31077752 |
| chr_2 | g8512 | g8512.t1 | cds | g8512.t1.CDS5 | 31077558 | 31077752 |
| chr_2 | g8512 | g8512.t1 | exon | g8512.t1.exon6 | 31077824 | 31077939 |
| chr_2 | g8512 | g8512.t1 | cds | g8512.t1.CDS6 | 31077824 | 31077939 |
| chr_2 | g8512 | g8512.t1 | exon | g8512.t1.exon7 | 31078013 | 31078161 |
| chr_2 | g8512 | g8512.t1 | cds | g8512.t1.CDS7 | 31078013 | 31078161 |
| chr_2 | g8512 | g8512.t1 | exon | g8512.t1.exon8 | 31078218 | 31078274 |
| chr_2 | g8512 | g8512.t1 | cds | g8512.t1.CDS8 | 31078218 | 31078274 |
| chr_2 | g8512 | g8512.t1 | exon | g8512.t1.exon9 | 31078338 | 31078446 |
| chr_2 | g8512 | g8512.t1 | cds | g8512.t1.CDS9 | 31078338 | 31078446 |
| chr_2 | g8512 | g8512.t1 | exon | g8512.t1.exon10 | 31078506 | 31078691 |
| chr_2 | g8512 | g8512.t1 | cds | g8512.t1.CDS10 | 31078506 | 31078691 |
| chr_2 | g8512 | g8512.t1 | exon | g8512.t1.exon11 | 31078765 | 31078849 |
| chr_2 | g8512 | g8512.t1 | cds | g8512.t1.CDS11 | 31078765 | 31078849 |
| chr_2 | g8512 | g8512.t1 | TTS | g8512.t1 | 31079109 | 31079109 |
Sequences
>g8512.t1 Gene=g8512 Length=3237
ATGGATATTTCTGAAAATTCCAAGAATAAATTTGTCGAAAACATTCGTAAAAATCGAAGT
GATAGACATCAAGAAAAGGAAAAGGAGGAAAGAAGGCAACATGCTGCTATTCTAATTCAA
ACTATATATCGAGGCTTTATTGCGAGGAAAAAGTTTCGTGATGAAATTATCAAAAATTTC
AACGAAATTATTCCCGATTCTATTGATAAAGAAACAATTGAGCTGAAACCTGCTCTTCAA
ATTTATCATGCACTTTCACGTTTTCTATTAATATGGAAACCTAAAGAAGATGATGAAGAA
AGCAGAGAAAAATGGGAAACAATTTGTAGAATTTTGTTAGCCAGTGTTGAGTCAGAATCA
CTTAAATTTAATTACGTTTCTGTGGCATTAAATAAGGACCATAGTCTTGCATGGATAAGA
CACATGAAAAAAGTTCTTTTCTATTGTTGCACATTTATTGATTCATTAAAACCAGAAATT
CACAAGGATACAACATTACTTAATGTGTTGCTAAGGACTTTGATTGCATTCACATGTCCA
AACGGCTGGGCAATTTTAAAAACAAAGCAATTAGCACCAATGAAGCCTGCTATGCAAACA
ATTTGCAACAATATTGTGGGATTTCTTATTCAAAAAGGCTTCTATTTAACACTTCGAACG
ATATTAGTAAAAGGAACTTGCCGTACCGTGGTGACACTCAAACCAATTTCAATTAATGCT
ATAATGTCATTAGCTATGCGATCACTTGTAGCTTCAAATTTTTCAGAAAATTTACTAACA
CAATTTGTATGCCAAATACTTTCAATACCCGCTCTTGTTTATCATTTAGTTGAATTTTGT
CCAGATATTCTAAAGAATTTTCAATCACGTGATTTATTTGAAAAGTGTATTGATATGATT
TCTGACAAGGACAATTTTAAATTCGTTTCTGGTTCACTTCAAGGTGCAAAATTGCTTGCA
TTAACAGCAAATTTAATTCAACTTTATTCAATGGAACCACCTGAAAAAGCACAAAATTTA
GCTTATCCACAAATTGTAAATTGTTTGAAAATGCTATTTGAAACAATTCCAGATAAGGAA
AATCAAAGAGGAACTTGTAGTCAATGGCATGAATTGTTAGGATGGTACAATGTAAAATCA
GGCGATACAGTTCATGAAAATTTATCTTTAATAAGAAAACAAATGAATCTCTTATGGGGT
CATAAAATGACTAAAATTCTTCTTGGTGATTTTCTAAAAACAGTAGCAGAATCATTGGAA
AAAAGCAATGAAAAAGATGGAAGTATGTCACCAGGAAATAGTTCATCTAGCACAAATTCA
TTTATAAAGAAAGCTATTGAACTTAAGAGTGGTATAATTAACAAAAGTTCTAAAAATTTC
AAAAAATTCGAGTCATTTGAAATTCAACGAATATCGACAACATGTGCAATGTATTATGGA
TCTTTAATAGCACTTCCACAATTAAAACTTGACATTTTATCGGGAATTTGCTACACTGGA
ACTATTTTGCGTGATTTGTGGACATTAATAAATTCACTTGGGATGAATTCATTTCATTCT
CTCATTCGCTACGACGACATTAACAATCCACCAGGTCTTTTATTACTCTTGTTTTGTGAT
GCGATGACACATTATGTCACGATACTGGATGACATTGAAATGTATGAACAACAGATGTTA
TTTACATTACAAGACTACACTGTTTTGTCATATTTTCTCAACAATTTGCTTTTTAAGACT
GTTAATGATAACATCTATGAAGCAAAAGTTGTTTTCAATTGTCCTTTATTTGTGTCCATG
CATACACTTTTATTATGCTTATATCGACGTGATAATCGACGACGCTTTGTTGCAAGCGAT
AATCATTGGATCATTAAAGAAATAAAGCCAGCGCACTTTTTGATGGACCTTGAAAAAGGA
AAGAGCAAACACCCTCAGACACAGTTGTTATTACAAAAAATGCCTCACATAATATCCCAT
CAAGAACGAGTTGCACTTTTCCGTAAATATGTTCAAAACGAAAAGAGCGTTCTTGGTTTA
TGTGATTCTGCATATGCCTCAAGTTCTGCACTTGTAACCGTTCATCGTGATAGAATTGTC
GAAGATGGATATAGACAATTAGCTCAGTTGCCAGCTAGAGCATTGAAAGGAATTATACGA
GTTCGTTTTATAAATAGTCAAGGTCTAGATGAAGCCGGAATTGATCAGGATGGAGTTTTC
AAAGAATTCTTAGAAGAAACAATCAAAAAAGTTTTTGATCCATCGCTTAATCTCTTTAAA
ACGACTGCTGAAAATCGTCTGTATCCATCACCAACATCGTATTTGCAAGAAAATCATTTG
CAACTTTTTGAATTTGTTGGAAAAATGCTCGGTAAAATGGTTTATGAGGGAATATTAGTT
GAAGTTCCATTTGCGTCATTTTTCCTGTCTCTGGTTCTTGGTCAAACACAGCAAGCACTT
TATTCATGTTTGGATGAGCTTGGATCATTAGATAATGAGTTATATAGGAGTCTTTCGTTT
ATTAAACATTATGAGGGTGATGTGTCTGATTTAGATTTGACTTTTTCTGTTGACGAAGAT
GTTATGGGAAAGGTGGTTACACATGAGTTATTTCCAGGTGGAAAAATGACGGCAGTCACA
AATGAAAATAGAATAAATTATATACACAGCATGGCATATTTCCGAATGCATACACAAATT
TGTGAACAAACAAATGCTTTTATTCGTGGCTTCCGTAGCATCATCAATCCTGATTGGCTT
TCGTTATTTTCCACAAATGAATTACAGCGGCTCATTTCTGGTGACACGAGTCCATTTGAT
TTAAAAGATTTACGACGACACACAAGTTATTTTGGCGGCTTTCATAATGATCATCGGGTA
ATAAATTGGTTATGGGATATCCTTGCAAAAGATTTTACTGAAGAAGAACGTCGCTTGTTT
CTCAAGTTTGTCACAAGCTGTTCAAGACCGCCTCTTCTCGGATTTTCTCATATGGAACCT
CAATTTAGTATTCGTTGTGTTGAAGTAAGTGAAGAAGAAGACACTGGAGATACAATCGGC
TCAGTCATAAGAGGATTCTTTACGATTCGCAAAAAAGAGCCAGCAATACAAACACGATTA
CCAACAAGCTCAACTTGCTTTAATTTATTAAAGTTGCCGAATTATCAAAAGAAGAGTATT
CTGAGAGACAAATTGAGATATGCCATAACAAGTAATACAGGATTTGAATTGTCGTAA
>g8512.t1 Gene=g8512 Length=1078
MDISENSKNKFVENIRKNRSDRHQEKEKEERRQHAAILIQTIYRGFIARKKFRDEIIKNF
NEIIPDSIDKETIELKPALQIYHALSRFLLIWKPKEDDEESREKWETICRILLASVESES
LKFNYVSVALNKDHSLAWIRHMKKVLFYCCTFIDSLKPEIHKDTTLLNVLLRTLIAFTCP
NGWAILKTKQLAPMKPAMQTICNNIVGFLIQKGFYLTLRTILVKGTCRTVVTLKPISINA
IMSLAMRSLVASNFSENLLTQFVCQILSIPALVYHLVEFCPDILKNFQSRDLFEKCIDMI
SDKDNFKFVSGSLQGAKLLALTANLIQLYSMEPPEKAQNLAYPQIVNCLKMLFETIPDKE
NQRGTCSQWHELLGWYNVKSGDTVHENLSLIRKQMNLLWGHKMTKILLGDFLKTVAESLE
KSNEKDGSMSPGNSSSSTNSFIKKAIELKSGIINKSSKNFKKFESFEIQRISTTCAMYYG
SLIALPQLKLDILSGICYTGTILRDLWTLINSLGMNSFHSLIRYDDINNPPGLLLLLFCD
AMTHYVTILDDIEMYEQQMLFTLQDYTVLSYFLNNLLFKTVNDNIYEAKVVFNCPLFVSM
HTLLLCLYRRDNRRRFVASDNHWIIKEIKPAHFLMDLEKGKSKHPQTQLLLQKMPHIISH
QERVALFRKYVQNEKSVLGLCDSAYASSSALVTVHRDRIVEDGYRQLAQLPARALKGIIR
VRFINSQGLDEAGIDQDGVFKEFLEETIKKVFDPSLNLFKTTAENRLYPSPTSYLQENHL
QLFEFVGKMLGKMVYEGILVEVPFASFFLSLVLGQTQQALYSCLDELGSLDNELYRSLSF
IKHYEGDVSDLDLTFSVDEDVMGKVVTHELFPGGKMTAVTNENRINYIHSMAYFRMHTQI
CEQTNAFIRGFRSIINPDWLSLFSTNELQRLISGDTSPFDLKDLRRHTSYFGGFHNDHRV
INWLWDILAKDFTEEERRLFLKFVTSCSRPPLLGFSHMEPQFSIRCVEVSEEEDTGDTIG
SVIRGFFTIRKKEPAIQTRLPTSSTCFNLLKLPNYQKKSILRDKLRYAITSNTGFELS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g8512.t1 | CDD | cd00078 | HECTc | 692 | 1076 | 2.26127E-145 |
| 6 | g8512.t1 | Gene3D | G3DSA:3.90.1750.10 | Hect | 692 | 934 | 5.2E-78 |
| 7 | g8512.t1 | Gene3D | G3DSA:3.30.2160.10 | Hect | 815 | 899 | 5.2E-78 |
| 8 | g8512.t1 | Gene3D | G3DSA:3.30.2410.10 | Hect | 937 | 1078 | 6.9E-36 |
| 17 | g8512.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 12 | 32 | - |
| 3 | g8512.t1 | PANTHER | PTHR45700:SF3 | UBIQUITIN-PROTEIN LIGASE E3B | 4 | 1078 | 0.0 |
| 4 | g8512.t1 | PANTHER | PTHR45700 | UBIQUITIN-PROTEIN LIGASE E3C | 4 | 1078 | 0.0 |
| 1 | g8512.t1 | Pfam | PF00612 | IQ calmodulin-binding motif | 34 | 53 | 1.7E-4 |
| 2 | g8512.t1 | Pfam | PF00632 | HECT-domain (ubiquitin-transferase) | 748 | 1078 | 3.1E-91 |
| 11 | g8512.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 589 | - |
| 13 | g8512.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 590 | 608 | - |
| 9 | g8512.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 609 | 792 | - |
| 12 | g8512.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 793 | 813 | - |
| 10 | g8512.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 814 | 1078 | - |
| 18 | g8512.t1 | ProSiteProfiles | PS50096 | IQ motif profile. | 32 | 61 | 9.633 |
| 19 | g8512.t1 | ProSiteProfiles | PS50237 | HECT domain profile. | 711 | 1078 | 77.137 |
| 16 | g8512.t1 | SMART | SM00015 | iq_5 | 31 | 53 | 2.4E-4 |
| 15 | g8512.t1 | SMART | SM00119 | hect_3 | 704 | 1078 | 1.7E-106 |
| 5 | g8512.t1 | SUPERFAMILY | SSF56204 | Hect, E3 ligase catalytic domain | 687 | 1071 | 1.11E-104 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005515 | protein binding | MF |
| GO:0004842 | ubiquitin-protein transferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K10588 (ubiquitin-protein ligase E3 B)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
