General information for g8528.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Ubiquitin-conjugating enzyme E2-22 kDa.
Parent gene
- The parent gene g8528 is located on chr_2: 31129880..31130377 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8528 | g8528.t1 | TSS | g8528.t1 | 31111177 | 31111177 |
| chr_2 | g8528 | g8528.t1 | isoform | g8528.t1 | 31129880 | 31130377 |
| chr_2 | g8528 | g8528.t1 | exon | g8528.t1.exon1 | 31129880 | 31129971 |
| chr_2 | g8528 | g8528.t1 | cds | g8528.t1.CDS1 | 31129880 | 31129971 |
| chr_2 | g8528 | g8528.t1 | exon | g8528.t1.exon2 | 31130039 | 31130159 |
| chr_2 | g8528 | g8528.t1 | cds | g8528.t1.CDS2 | 31130039 | 31130159 |
| chr_2 | g8528 | g8528.t1 | exon | g8528.t1.exon3 | 31130219 | 31130377 |
| chr_2 | g8528 | g8528.t1 | cds | g8528.t1.CDS3 | 31130219 | 31130377 |
| chr_2 | g8528 | g8528.t1 | TTS | g8528.t1 | 31130426 | 31130426 |
Sequences
>g8528.t1 Gene=g8528 Length=372
ATGTTGGTGTGGGCTGATATGGATGATGATGGTGTTGATGAAGAAGGAGAGAATGATGAT
GAAAAAGAAAATCAAATGAATGGCATATTTTTAGAACTGTCTACACTAAGCCTTCAAGCT
CTTCTCGCCGCTGCTGAACCAGATGATCCTCAAGATGCTGTTGTTGCTAATCAATATAAG
AAAAATTACGAAATGTTTTGTAAAACTGCTAAGCATTGGACAAATGTTTATGCAAAAGGC
CCACATAAAATTCCAGAATTTGATACAAAAGTGCAGACTCTTAAAAACATAGGAGTAAAT
GAATTTGATGCAAGAGCTGCGTTAAGTCGACATAATTGGGATATTAATCTAGCAACCAAT
GATATATTCTGA
>g8528.t1 Gene=g8528 Length=123
MLVWADMDDDGVDEEGENDDEKENQMNGIFLELSTLSLQALLAAAEPDDPQDAVVANQYK
KNYEMFCKTAKHWTNVYAKGPHKIPEFDTKVQTLKNIGVNEFDARAALSRHNWDINLATN
DIF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g8528.t1 | Gene3D | G3DSA:3.10.110.10 | Ubiquitin Conjugating Enzyme | 23 | 82 | 2.4E-11 |
| 8 | g8528.t1 | Gene3D | G3DSA:1.10.8.10 | DNA helicase RuvA subunit | 87 | 123 | 4.6E-5 |
| 5 | g8528.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 26 | - |
| 6 | g8528.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 7 | 21 | - |
| 1 | g8528.t1 | PANTHER | PTHR24068:SF164 | UBIQUITIN-CONJUGATING ENZYME E2 21-RELATED | 34 | 117 | 4.5E-18 |
| 2 | g8528.t1 | PANTHER | PTHR24068 | UBIQUITIN-CONJUGATING ENZYME E2 | 34 | 117 | 4.5E-18 |
| 9 | g8528.t1 | ProSiteProfiles | PS50030 | Ubiquitin-associated domain (UBA) profile. | 86 | 123 | 8.595 |
| 3 | g8528.t1 | SUPERFAMILY | SSF54495 | UBC-like | 36 | 85 | 2.06E-7 |
| 4 | g8528.t1 | SUPERFAMILY | SSF46934 | UBA-like | 70 | 123 | 5.11E-6 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005515 | protein binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
