General information for g8541.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Pyrimidodiazepine synthase.
Parent gene
- The parent gene g8541 is located on chr_2: 31195530..31196506 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8541 | g8541.t3 | isoform | g8541.t3 | 31194871 | 31196452 |
| chr_2 | g8541 | g8541.t3 | exon | g8541.t3.exon1 | 31194871 | 31194884 |
| chr_2 | g8541 | g8541.t3 | exon | g8541.t3.exon2 | 31195532 | 31196053 |
| chr_2 | g8541 | g8541.t3 | cds | g8541.t3.CDS1 | 31195582 | 31196053 |
| chr_2 | g8541 | g8541.t3 | exon | g8541.t3.exon3 | 31196110 | 31196452 |
| chr_2 | g8541 | g8541.t3 | cds | g8541.t3.CDS2 | 31196110 | 31196297 |
| chr_2 | g8541 | g8541.t3 | TTS | g8541.t3 | 31196549 | 31196549 |
| chr_2 | g8541 | g8541.t3 | TSS | g8541.t3 | NA | NA |
Sequences
>g8541.t3 Gene=g8541 Length=879
CAGGTAAAATTTAGGTTCAACTCAACCAACTTTTCCTGATGATCAAAAGCTTCGACTTTA
TTCAATGAGATTTTGTCCTTTCGCTCAACGTGCTCATCTCGTGCTCGATGCAAAAAATAT
TCCGTATCATACTGCCTTTATTAATTTAACAGAAAAGCCGGAATGGTTTACTAAAGTTTC
TGCTTTGTCAAAAGTTCCAGCACTTGAAATTCCAGAAAGTGGCGATGTACTCATTGAATC
TTTGATAATTGCTGACTATCTCGATGAAAAATATCCTCAAAATCCATTACACTCAAAAAA
TCCATTAGAAAAGGCTCGAGATAGAATTCTTATTGAAAGATTTAATGGATTTACTGCTCC
ATTTGCTCGAATTATATATAACCATAAGAAAGATGGAGCTCCGGGTGCGATTCATGAAAT
AGTCACAATTTTAGACTTGTTTGAAGAAGAACTTAAAAAGAGAGGAGCAAAATTCTTTGG
TGGTTCCAAACCAGGAATGCTCGATTATATGATTTGGCCTTGGTGTGAACGAACAGATAT
GTTAAAATATATGTTGGGCGATAAATATGAACTTGATAAAGTTCGATTTAGTGAACTCAT
TAAATGGAAGGAGGAAATGAAAAAAGACAAAGCCGTTAAGGAACATTATATTTCAGCCGA
AGATCATTATAAATTTATATCATTGAGACTTGCAAATACTCCTGATTATGATTTTCTGGC
GTAAGTTCCTACTTTCTGTCGAAACTTGTCCTTTCTCAAATTCTGAATTTTCTAATCACT
TTCGCTTTTATTGTCTTTTAACAAATTAATTTAAAAGTCGTTATTTTGTATTATCTTCAT
CGTAGGCCAACGGCTGCAAAAAAAATTCGCTTATCATAG
>g8541.t3 Gene=g8541 Length=219
MRFCPFAQRAHLVLDAKNIPYHTAFINLTEKPEWFTKVSALSKVPALEIPESGDVLIESL
IIADYLDEKYPQNPLHSKNPLEKARDRILIERFNGFTAPFARIIYNHKKDGAPGAIHEIV
TILDLFEEELKKRGAKFFGGSKPGMLDYMIWPWCERTDMLKYMLGDKYELDKVRFSELIK
WKEEMKKDKAVKEHYISAEDHYKFISLRLANTPDYDFLA
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g8541.t3 | Gene3D | G3DSA:3.40.30.10 | Glutaredoxin | 1 | 205 | 0.000 |
| 8 | g8541.t3 | Gene3D | G3DSA:1.20.1050.10 | - | 79 | 199 | 0.000 |
| 3 | g8541.t3 | PANTHER | PTHR43968 | - | 1 | 217 | 0.000 |
| 5 | g8541.t3 | PRINTS | PR01625 | Omega-class glutathione S-transferase signature | 70 | 84 | 0.000 |
| 4 | g8541.t3 | PRINTS | PR01625 | Omega-class glutathione S-transferase signature | 137 | 156 | 0.000 |
| 2 | g8541.t3 | Pfam | PF13417 | Glutathione S-transferase, N-terminal domain | 1 | 74 | 0.000 |
| 1 | g8541.t3 | Pfam | PF13410 | Glutathione S-transferase, C-terminal domain | 118 | 164 | 0.000 |
| 11 | g8541.t3 | ProSiteProfiles | PS50404 | Soluble glutathione S-transferase N-terminal domain profile. | 1 | 74 | 18.084 |
| 10 | g8541.t3 | ProSiteProfiles | PS50405 | Soluble glutathione S-transferase C-terminal domain profile. | 79 | 217 | 16.574 |
| 12 | g8541.t3 | SFLD | SFLDG00358 | Main (cytGST) | 1 | 187 | 0.000 |
| 13 | g8541.t3 | SFLD | SFLDS00019 | Glutathione Transferase (cytosolic) | 1 | 187 | 0.000 |
| 6 | g8541.t3 | SUPERFAMILY | SSF52833 | Thioredoxin-like | 2 | 96 | 0.000 |
| 7 | g8541.t3 | SUPERFAMILY | SSF47616 | GST C-terminal domain-like | 78 | 217 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004364 | glutathione transferase activity | MF |
| GO:0006749 | glutathione metabolic process | BP |
| GO:0005515 | protein binding | MF |
| GO:0005737 | cytoplasm | CC |
KEGG
Orthology
- This transcript has been annotated with K21888 (glutathione dehydrogenase/transferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
