Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Pyrimidodiazepine synthase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g8541 g8541.t4 TSS g8541.t4 31195210 31195210
chr_2 g8541 g8541.t4 isoform g8541.t4 31195315 31196035
chr_2 g8541 g8541.t4 exon g8541.t4.exon1 31195315 31195339
chr_2 g8541 g8541.t4 cds g8541.t4.CDS1 31195315 31195339
chr_2 g8541 g8541.t4 exon g8541.t4.exon2 31195532 31196035
chr_2 g8541 g8541.t4 cds g8541.t4.CDS2 31195532 31196034
chr_2 g8541 g8541.t4 TTS g8541.t4 31196549 31196549

Sequences

>g8541.t4 Gene=g8541 Length=529
ATGAGTAAATTACATTTATCAAAAGGTTCAACTCAACCAACTTTTCCTGATGATCAAAAG
CTTCGACTTTATTCAATGAGATTTTGTCCTTTCGCTCAACGTGCTCATCTCGTGCTCGAT
GCAAAAAATATTCCGTATCATACTGCCTTTATTAATTTAACAGAAAAGCCGGAATGGTTT
ACTAAAGTTTCTGCTTTGTCAAAAGTTCCAGCACTTGAAATTCCAGAAAGTGGCGATGTA
CTCATTGAATCTTTGATAATTGCTGACTATCTCGATGAAAAATATCCTCAAAATCCATTA
CACTCAAAAAATCCATTAGAAAAGGCTCGAGATAGAATTCTTATTGAAAGATTTAATGGA
TTTACTGCTCCATTTGCTCGAATTATATATAACCATAAGAAAGATGGAGCTCCGGGTGCG
ATTCATGAAATAGTCACAATTTTAGACTTGTTTGAAGAAGAACTTAAAAAGAGAGGAGCA
AAATTCTTTGGTGGTTCCAAACCAGGAATGCTCGATTATATGATTTGGC

>g8541.t4 Gene=g8541 Length=176
MSKLHLSKGSTQPTFPDDQKLRLYSMRFCPFAQRAHLVLDAKNIPYHTAFINLTEKPEWF
TKVSALSKVPALEIPESGDVLIESLIIADYLDEKYPQNPLHSKNPLEKARDRILIERFNG
FTAPFARIIYNHKKDGAPGAIHEIVTILDLFEEELKKRGAKFFGGSKPGMLDYMIW

Protein features from InterProScan

Transcript Database ID Name Start End E.value
9 g8541.t4 Gene3D G3DSA:3.40.30.10 Glutaredoxin 1 96 0.000
8 g8541.t4 Gene3D G3DSA:1.20.1050.10 - 102 176 0.000
2 g8541.t4 PANTHER PTHR43968 - 1 176 0.000
4 g8541.t4 PRINTS PR01625 Omega-class glutathione S-transferase signature 19 34 0.000
3 g8541.t4 PRINTS PR01625 Omega-class glutathione S-transferase signature 95 109 0.000
5 g8541.t4 PRINTS PR01625 Omega-class glutathione S-transferase signature 162 176 0.000
1 g8541.t4 Pfam PF13417 Glutathione S-transferase, N-terminal domain 23 99 0.000
11 g8541.t4 ProSiteProfiles PS50404 Soluble glutathione S-transferase N-terminal domain profile. 19 99 20.094
10 g8541.t4 ProSiteProfiles PS50405 Soluble glutathione S-transferase C-terminal domain profile. 104 176 13.459
12 g8541.t4 SFLD SFLDG00358 Main (cytGST) 21 176 0.000
13 g8541.t4 SFLD SFLDS00019 Glutathione Transferase (cytosolic) 21 176 0.000
6 g8541.t4 SUPERFAMILY SSF52833 Thioredoxin-like 20 121 0.000
7 g8541.t4 SUPERFAMILY SSF47616 GST C-terminal domain-like 103 176 0.000

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0004364 glutathione transferase activity MF
GO:0006749 glutathione metabolic process BP
GO:0005515 protein binding MF
GO:0005737 cytoplasm CC

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values