General information for g8547.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Glutathione hydrolase 1 proenzyme.
Parent gene
- The parent gene g8547 is located on chr_2: 31227719..31228289 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8547 | g8547.t1 | TTS | g8547.t1 | 31225788 | 31225788 |
| chr_2 | g8547 | g8547.t1 | isoform | g8547.t1 | 31225841 | 31228289 |
| chr_2 | g8547 | g8547.t1 | exon | g8547.t1.exon1 | 31225841 | 31226151 |
| chr_2 | g8547 | g8547.t1 | cds | g8547.t1.CDS1 | 31225841 | 31226151 |
| chr_2 | g8547 | g8547.t1 | exon | g8547.t1.exon2 | 31226282 | 31226484 |
| chr_2 | g8547 | g8547.t1 | cds | g8547.t1.CDS2 | 31226282 | 31226484 |
| chr_2 | g8547 | g8547.t1 | exon | g8547.t1.exon3 | 31226536 | 31227183 |
| chr_2 | g8547 | g8547.t1 | cds | g8547.t1.CDS3 | 31226536 | 31227183 |
| chr_2 | g8547 | g8547.t1 | exon | g8547.t1.exon4 | 31227706 | 31228289 |
| chr_2 | g8547 | g8547.t1 | cds | g8547.t1.CDS4 | 31227706 | 31228289 |
| chr_2 | g8547 | g8547.t1 | TSS | g8547.t1 | 31228322 | 31228322 |
Sequences
>g8547.t1 Gene=g8547 Length=1746
ATGAAGCATCATATGAGTAAAACGACAGCTATTGCTACTGCCGTTCTTATCGTCTTTATC
ATTAGTTTAGGCATTCTGTTATGGTTTGCTTATCATCAAGGTCTAATTGATGATGGTTTG
TCAACAGCTGCTGTAGCTACAACGTCCGATGAGTGTACAGATGCTGCTGTATTAATTTTA
AAGCAAGGTGGTTCGGCTGCCGATGCTGCAATATGCGCATCATTATGCCAAGGTATAGTT
GTACCGCAGGCTAGCGGTATAGGAGGTGGATTTCTTGCAACCATATATACAAAAAACACT
GCCGAGATAGTAACATTGAATTCAAGAGAAGTTGCACCATTGAAAGCCACACAAGAACTT
TATTTAGATAACGAACGTGCTACCGTTGAAGGCGGACTTTCAATTGCTGTTCCTACTGAA
GTAAAAGGCTTACACGAACTCCATCGACGTTTCGGGAAATTGAGTTGGAGTCGTGTGCTT
GAACCGGCAATTCATGTTGCAGAAAAAGGCTTTAAAGTACCAAATTATTTGGCACGAGTG
TTGAGCCAATATGAAGAAAAGATAAGAAGGACTGACGGTTTCAAAGATTTCTATGTAAAT
CCAAAAACAAATGAAATTTATCGTGAGGGTGACACACTTAAAAATCCTCAATTGGCAGAA
ACATTAAAAATAATTGCGCGCGAGGGTGCGAATGCGATTTACAGCACTGAGGGTTCATTA
GGACAAAAACTTGTGAATGAAATTCAAGCAAATGGAGGCATTGTGACGATGAATGATTTG
CGAATTTATCAACCGAAATTCGCACCATCTGTCGCGGTGAAGCTTTTTCAAGGCGATACT
TTGCACACCACAAATCTTCCCTCATCCGGCAGCATTTTCGCATTTATTTTGAATATCCTC
GAGGGTTACAAATTTCACGAAAATTCTTTCGACTTTCATCGTGGTAATAAATTAATTTAC
CATCGCATTGTTGAAGCATTCAAATTTGGCTTTGGCTTTCGAACATCGCTTGGTGATGAA
ACGAGTGAAGAAGTAGTTGAAACATTACTGAATGTACTCGATGTTGATTTTGCTAACCAC
ATTAGAGCATTTATTAATGATGAACAAACATTCAATAGCAGCGAATATTATCGTGCAAAT
ACCTCAGTCACGATTGACCACGGAACAGGTCACATTTCTATCCTAGCACCAAATGGTGAT
GCAGTGGCATTAACATCAACAATTAATTCAATATTTGGTTCATGGGTCATGTCAAATAGC
ACTGGAATAATCTTTAATAATGAAATGGACGATTTTTCACTTCCAACAGCATCAGATGGT
TTAATTCAATCTACAGCGAATTCAATTAAAGCAGGAAAAAGTCCGATGTCATCTATGATA
CCAATGATACTTGTAAATGAGAAACACGATGCTACACTGATTGTAGGTGGTGCAGGTGGA
ATCCGTATAATGACCTCATTAGTTCTTTTTCTAATTAACCACTTGTATCTAAAGTTATCA
ATGGAAGAAGCTTTAGAAATGAAAAGATTTCATCATCAATTGCAGCCAATGCGTGTTCAA
TTTGAAGATGGTTATGATCAACCAGATATTGTCGAGTATTTACGTCAAAAAGGTCATGCA
ACTGTCGAAGCAGGTCCATTACTTTCAGCATTTGCATCAATTATTGCGATTTCAAATCGA
AACGGTCGAGTTGAGTTTGCTATAGACCCTAGACGAGGAGGTAAAGGTGTAATTTTTGAT
CCGTAA
>g8547.t1 Gene=g8547 Length=581
MKHHMSKTTAIATAVLIVFIISLGILLWFAYHQGLIDDGLSTAAVATTSDECTDAAVLIL
KQGGSAADAAICASLCQGIVVPQASGIGGGFLATIYTKNTAEIVTLNSREVAPLKATQEL
YLDNERATVEGGLSIAVPTEVKGLHELHRRFGKLSWSRVLEPAIHVAEKGFKVPNYLARV
LSQYEEKIRRTDGFKDFYVNPKTNEIYREGDTLKNPQLAETLKIIAREGANAIYSTEGSL
GQKLVNEIQANGGIVTMNDLRIYQPKFAPSVAVKLFQGDTLHTTNLPSSGSIFAFILNIL
EGYKFHENSFDFHRGNKLIYHRIVEAFKFGFGFRTSLGDETSEEVVETLLNVLDVDFANH
IRAFINDEQTFNSSEYYRANTSVTIDHGTGHISILAPNGDAVALTSTINSIFGSWVMSNS
TGIIFNNEMDDFSLPTASDGLIQSTANSIKAGKSPMSSMIPMILVNEKHDATLIVGGAGG
IRIMTSLVLFLINHLYLKLSMEEALEMKRFHHQLQPMRVQFEDGYDQPDIVEYLRQKGHA
TVEAGPLLSAFASIIAISNRNGRVEFAIDPRRGGKGVIFDP
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 16 | g8547.t1 | Gene3D | G3DSA:1.10.246.130 | - | 271 | 380 | 3.6E-29 |
| 15 | g8547.t1 | Gene3D | G3DSA:3.60.20.40 | - | 384 | 578 | 3.4E-59 |
| 2 | g8547.t1 | PANTHER | PTHR11686 | GAMMA GLUTAMYL TRANSPEPTIDASE | 7 | 574 | 1.1E-179 |
| 3 | g8547.t1 | PANTHER | PTHR11686:SF51 | GAMMA-GLUTAMYL TRANSPEPTIDASE, ISOFORM A | 7 | 574 | 1.1E-179 |
| 7 | g8547.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 65 | 90 | 7.3E-64 |
| 10 | g8547.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 134 | 152 | 7.3E-64 |
| 9 | g8547.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 152 | 171 | 7.3E-64 |
| 8 | g8547.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 254 | 270 | 7.3E-64 |
| 11 | g8547.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 284 | 303 | 7.3E-64 |
| 4 | g8547.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 389 | 407 | 7.3E-64 |
| 12 | g8547.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 413 | 431 | 7.3E-64 |
| 5 | g8547.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 451 | 466 | 7.3E-64 |
| 6 | g8547.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 476 | 493 | 7.3E-64 |
| 1 | g8547.t1 | Pfam | PF01019 | Gamma-glutamyltranspeptidase | 59 | 574 | 2.2E-153 |
| 17 | g8547.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 11 | - |
| 21 | g8547.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 12 | 31 | - |
| 19 | g8547.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 32 | 470 | - |
| 20 | g8547.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 471 | 492 | - |
| 18 | g8547.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 493 | 581 | - |
| 14 | g8547.t1 | SUPERFAMILY | SSF56235 | N-terminal nucleophile aminohydrolases (Ntn hydrolases) | 42 | 576 | 8.1E-138 |
| 13 | g8547.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 9 | 31 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006751 | glutathione catabolic process | BP |
| GO:0036374 | glutathione hydrolase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K18592 (gamma-glutamyltranspeptidase / glutathione hydrolase / leukotriene-C4 hydrolase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed