General information for g8567.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Dual specificity mitogen-activated protein kinase kinase hemipterous.
Parent gene
- The parent gene g8567 is located on chr_2: 31369927..31373718 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8567 | g8567.t2 | TTS | g8567.t2 | 31365390 | 31365390 |
| chr_2 | g8567 | g8567.t2 | isoform | g8567.t2 | 31365945 | 31369927 |
| chr_2 | g8567 | g8567.t2 | exon | g8567.t2.exon1 | 31365945 | 31366089 |
| chr_2 | g8567 | g8567.t2 | cds | g8567.t2.CDS1 | 31365945 | 31366089 |
| chr_2 | g8567 | g8567.t2 | exon | g8567.t2.exon2 | 31366150 | 31366462 |
| chr_2 | g8567 | g8567.t2 | cds | g8567.t2.CDS2 | 31366150 | 31366462 |
| chr_2 | g8567 | g8567.t2 | exon | g8567.t2.exon3 | 31366725 | 31367932 |
| chr_2 | g8567 | g8567.t2 | cds | g8567.t2.CDS3 | 31366725 | 31367932 |
| chr_2 | g8567 | g8567.t2 | exon | g8567.t2.exon4 | 31368501 | 31368852 |
| chr_2 | g8567 | g8567.t2 | cds | g8567.t2.CDS4 | 31368501 | 31368852 |
| chr_2 | g8567 | g8567.t2 | exon | g8567.t2.exon5 | 31368920 | 31369145 |
| chr_2 | g8567 | g8567.t2 | cds | g8567.t2.CDS5 | 31368920 | 31369145 |
| chr_2 | g8567 | g8567.t2 | exon | g8567.t2.exon6 | 31369207 | 31369700 |
| chr_2 | g8567 | g8567.t2 | cds | g8567.t2.CDS6 | 31369207 | 31369700 |
| chr_2 | g8567 | g8567.t2 | exon | g8567.t2.exon7 | 31369849 | 31369854 |
| chr_2 | g8567 | g8567.t2 | cds | g8567.t2.CDS7 | 31369849 | 31369854 |
| chr_2 | g8567 | g8567.t2 | exon | g8567.t2.exon8 | 31369906 | 31369927 |
| chr_2 | g8567 | g8567.t2 | cds | g8567.t2.CDS8 | 31369906 | 31369927 |
| chr_2 | g8567 | g8567.t2 | TSS | g8567.t2 | 31369942 | 31369942 |
Sequences
>g8567.t2 Gene=g8567 Length=2766
ATGAAAAAAAAATTTGTAAAAACGATAATGTCAAATTTGAATATAAATTTGACACCAGTC
AATAAAAAACGACCTATACCTCAGCTTCCAATTCCGACCCTCTCACAGAGACCTAGAAAT
CAGGATAATGAGAAAAAATTGCGAGCCTTGAGGACTAATACACTAAAAATAAATGGAATA
AATTATGAAACAAATATCCAAGACTTGGAATATCTTAGCGAATTAGGAAATGGCACGTCT
GGTCATGTGGTCAAAATGAAACACAAGCCTAGTGGTGCTGTGATTGCAGTGAAACAGATG
CGTAGGACAGGCAATGATGAAGAAACAAAAAGAATAACGATGGACTTGGATGTTGTGCTT
AAATCACACGACTGTCCTTTCATCGTGCAATGTTTAGGATGTTTTATAACTGAAGCAGAT
GTGTGGATTTGTATGGAACTTATGTCCACATGCTTCGATAAACTACAAAAACGAACCCAA
AAACCAATTCCCGAGTATATTCTAAAATGTGTAACTGAAGCTACTGTGAATGCATTAAAT
TATCTGAAAATTAAACATGGAGTTATTCATAGAGATGTCAAACCTAGTAATATTCTAATA
GATGAACATGGAATAATTAAACTATGTGACTTTGGAATAAGTGGTCGTTTAGTTGACTCA
AAAGCAAAAACACGCTCAGCTGGCTGCGCAGCATATATGGCTCCCGAAAGAATCGATCCA
AAAAAACCTGAGTACGATATTCGTGCAGATGTTTGGTCATTAGGGATAACATTAGTTGAA
TTGGCTACAGGAGCGTACCCTTATAAAGACTGCAAAACTGACTTCGAAGTGCTCACTAAA
GTATTAGATTCCGAACCGCCTTCACTGCCTGATGATCAAGGTTTTAGTGAAGATTTTAAG
GACTTTGTAAAGCGATGCCTAACGAAGGACTATAAACAACGACCTAAATATAATCAACTG
CTAGAACATCCTTTCCTTAATAATCCTGATCCCCAAAAATTAGTTAATGTATCCGCATGG
TTTCATGCTGTAACTAAAGAAGCTGGAATTGTCTTACCAACACAAGATTCGCCAAATCTA
ACTGCCTCGCATTTAAATAGAATTTCCTCACTTCAATCAGTCTCAGTCTCATCAACGCAA
ATACAGCAGCATCAACAGCCTTTAGTTATGGCAAAAGAAAGTAGTAGTGTAACAGATGGT
AATAACTTTTCATCTATGAATATGAATCCTTTTTCTCCTCCTTCTTCTACGACTTCATTC
ACTTCGCCTCAATTTCAAATGAATAACACAATTGAACGTGAGATGTCAACGACAAATAGA
AATATCGAAAATATCTTTCCATGTACGTCAAACTATCAACAACAACAGCAGCAGCAAGCG
GCATCGATTTATGGCAAAATAAATGATCCAATTAATAGTAGCGATATGGATTATCGTAGT
GGCAATGGTTATCAAAAGTTGACATCAAATAATTTCAATGATGGAAATATTGATTATGAG
CTGCTATCAAAATTTAATCGAATGCATACACAATCACCACAACTGCCTCGTAAGAATTAT
GCACTATTAAGAAATGTCAACTCGCCACCATATCTTCATGAAGGATCACAATCACCTCTT
ATGCATCCAAAATTACAATATGATGATAGAAATAATTTTCATTATCAACATAGTCCTTAT
AAATCAAATGTTACAGCTTCAGCTGGCACTTATTCACCAGTTGTAAAAAAACGCTATGGA
CAAGATGGTGTAATGGTAGCAGAAGATTTAGAATTTAGAATTTTACATGGAAATACTAGT
CCAATTGTGCTACAACGATTCTATCATCAACAAAATCAACTGCGAGATCAAAAAGAAGAG
CAATTAAGGGCAATGAAAAATTATAATTCATCAAGTACAAATCCTTTTGAACAACACAAC
TTTGCTCAATCAAATATTCCAATCGCGACTCATCATCATCACAATTCACCTTCATTCATG
AAATACACATCGTCATCGCCTACACCGCAGAGAAATACAAACTTTTACGAAAACAATTAC
AATAATGCTAGTCCAAGTTATATTCCTCAATATACAAAGAATACTAACGGAAATGGAACA
TATGCTCGATATCAGCCAAATATTAATCATTCGAATTTAGCTAATCGACCGCCAATTCCT
TGTCCAAATTCTCCTCAACTTGATCGACTTCGTGCAAATCTAGAAAAGCCCAATTTTTAT
GAACGACATCAACTGCCAGTTGAAGTACAATTAAAGGAAAATGTCACAACTTCTTCATCT
GGCAATCAGTCAAATGAAAAAAGTAAAGGATGGCTAAATTCTGTTTCAGGCATGGTAAAG
ACTTATATCAAAACTCGATTGCACATTGATCAAAATAATAAAGATGGTGAGAAAGTTAAA
TCATTGGCAACAATATCGCCAATACGTTCAGCGACAAATCCATTCGCTGCTCAATCGCCA
GCTGCCAATAATAAAACTACTACTAATCTTATATCATCAACTGCTTCATTCGATCGACAT
CGTAGCCCTGATCCACCAATGAGAACGCAAAGAGGCAGTCAGAGTCCATTGATTCTAAGA
AAAAAATTGGAAATGGCTAGTTCACCTATTCTACAAAGACGATATATGTCAGCATCACCG
TCACCACCACCAATAGTTCCACGCATGAGACCAACATATAATAATAATGATTTAGGAAGT
TCACCTCATCATTATCATGCAAGAATCAATTATACTCCAGAACCACAGCGACGAATATAT
CAATGA
>g8567.t2 Gene=g8567 Length=921
MKKKFVKTIMSNLNINLTPVNKKRPIPQLPIPTLSQRPRNQDNEKKLRALRTNTLKINGI
NYETNIQDLEYLSELGNGTSGHVVKMKHKPSGAVIAVKQMRRTGNDEETKRITMDLDVVL
KSHDCPFIVQCLGCFITEADVWICMELMSTCFDKLQKRTQKPIPEYILKCVTEATVNALN
YLKIKHGVIHRDVKPSNILIDEHGIIKLCDFGISGRLVDSKAKTRSAGCAAYMAPERIDP
KKPEYDIRADVWSLGITLVELATGAYPYKDCKTDFEVLTKVLDSEPPSLPDDQGFSEDFK
DFVKRCLTKDYKQRPKYNQLLEHPFLNNPDPQKLVNVSAWFHAVTKEAGIVLPTQDSPNL
TASHLNRISSLQSVSVSSTQIQQHQQPLVMAKESSSVTDGNNFSSMNMNPFSPPSSTTSF
TSPQFQMNNTIEREMSTTNRNIENIFPCTSNYQQQQQQQAASIYGKINDPINSSDMDYRS
GNGYQKLTSNNFNDGNIDYELLSKFNRMHTQSPQLPRKNYALLRNVNSPPYLHEGSQSPL
MHPKLQYDDRNNFHYQHSPYKSNVTASAGTYSPVVKKRYGQDGVMVAEDLEFRILHGNTS
PIVLQRFYHQQNQLRDQKEEQLRAMKNYNSSSTNPFEQHNFAQSNIPIATHHHHNSPSFM
KYTSSSPTPQRNTNFYENNYNNASPSYIPQYTKNTNGNGTYARYQPNINHSNLANRPPIP
CPNSPQLDRLRANLEKPNFYERHQLPVEVQLKENVTTSSSGNQSNEKSKGWLNSVSGMVK
TYIKTRLHIDQNNKDGEKVKSLATISPIRSATNPFAAQSPAANNKTTTNLISSTASFDRH
RSPDPPMRTQRGSQSPLILRKKLEMASSPILQRRYMSASPSPPPIVPRMRPTYNNNDLGS
SPHHYHARINYTPEPQRRIYQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g8567.t2 | CDD | cd06618 | PKc_MKK7 | 54 | 347 | 3.23789E-180 |
| 7 | g8567.t2 | Coils | Coil | Coil | 611 | 631 | - |
| 5 | g8567.t2 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 51 | 148 | 6.9E-38 |
| 6 | g8567.t2 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 149 | 347 | 6.2E-60 |
| 12 | g8567.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 835 | 854 | - |
| 13 | g8567.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 898 | 921 | - |
| 2 | g8567.t2 | PANTHER | PTHR47238:SF2 | DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE HEMIPTEROUS | 25 | 921 | 1.4E-194 |
| 3 | g8567.t2 | PANTHER | PTHR47238 | MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5 | 25 | 921 | 1.4E-194 |
| 1 | g8567.t2 | Pfam | PF00069 | Protein kinase domain | 71 | 326 | 4.9E-59 |
| 11 | g8567.t2 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 75 | 98 | - |
| 10 | g8567.t2 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 188 | 200 | - |
| 14 | g8567.t2 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 69 | 326 | 42.449 |
| 9 | g8567.t2 | SMART | SM00220 | serkin_6 | 69 | 326 | 7.2E-76 |
| 4 | g8567.t2 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 67 | 416 | 1.35E-71 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
- This transcript has been annotated with K04431 (mitogen-activated protein kinase kinase 7)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
