General information for g8590.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable beta-hexosaminidase fdl.
Parent gene
- The parent gene g8590 is located on chr_2: 31613577..31619467 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8590 | g8590.t1 | isoform | g8590.t1 | 31610577 | 31613577 |
| chr_2 | g8590 | g8590.t1 | exon | g8590.t1.exon1 | 31610577 | 31610734 |
| chr_2 | g8590 | g8590.t1 | cds | g8590.t1.CDS1 | 31610577 | 31610734 |
| chr_2 | g8590 | g8590.t1 | TSS | g8590.t1 | 31610606 | 31610606 |
| chr_2 | g8590 | g8590.t1 | exon | g8590.t1.exon2 | 31611361 | 31611839 |
| chr_2 | g8590 | g8590.t1 | cds | g8590.t1.CDS2 | 31611361 | 31611839 |
| chr_2 | g8590 | g8590.t1 | exon | g8590.t1.exon3 | 31611907 | 31612063 |
| chr_2 | g8590 | g8590.t1 | cds | g8590.t1.CDS3 | 31611907 | 31612063 |
| chr_2 | g8590 | g8590.t1 | exon | g8590.t1.exon4 | 31612121 | 31612404 |
| chr_2 | g8590 | g8590.t1 | cds | g8590.t1.CDS4 | 31612121 | 31612404 |
| chr_2 | g8590 | g8590.t1 | exon | g8590.t1.exon5 | 31612465 | 31612553 |
| chr_2 | g8590 | g8590.t1 | cds | g8590.t1.CDS5 | 31612465 | 31612553 |
| chr_2 | g8590 | g8590.t1 | exon | g8590.t1.exon6 | 31612613 | 31612742 |
| chr_2 | g8590 | g8590.t1 | cds | g8590.t1.CDS6 | 31612613 | 31612742 |
| chr_2 | g8590 | g8590.t1 | exon | g8590.t1.exon7 | 31612804 | 31612861 |
| chr_2 | g8590 | g8590.t1 | cds | g8590.t1.CDS7 | 31612804 | 31612861 |
| chr_2 | g8590 | g8590.t1 | exon | g8590.t1.exon8 | 31612924 | 31613260 |
| chr_2 | g8590 | g8590.t1 | cds | g8590.t1.CDS8 | 31612924 | 31613260 |
| chr_2 | g8590 | g8590.t1 | exon | g8590.t1.exon9 | 31613326 | 31613577 |
| chr_2 | g8590 | g8590.t1 | cds | g8590.t1.CDS9 | 31613326 | 31613577 |
| chr_2 | g8590 | g8590.t1 | TTS | g8590.t1 | NA | NA |
Sequences
>g8590.t1 Gene=g8590 Length=1944
ATGGCATTTACAACATCACTCAGAAGGCTTCTGATAGGTCTTTTATTTGTAATGGCATTA
TGTTTGATGTTATTATATTTTAATGAAAGTCAGAGTCTTCTGAAACCTGCGTCATCCTTT
CTCATCAATAGCTTTGATACAAGACACAATAAACTCAATCCATCAGAAAAAGCGTGGACG
TACAAATGTGTTAATCAAAAATGCGTTAGACAGCATTATTCAAATAAGAATGAAAAAAGA
GTTCCATTTACGTCATGTAGTATGCTATGTGGAAGTTACTCAAAAATTTGGCCAGAGCCA
ACAAAATCTTACATTGGAACAAGTGCCAATAGTTTTAATTTAAATGATGTTCAATTTAAA
ATCAAAACTCCTTTTAAGAATGTTGAGAATTTATTAGAAAGTGCATTTTCAATTTTTATG
GAAGAGATTAAGGATATTTTTCATTCGTCATCGGATCGAAAGACTGAAGAAGTTAGTAAG
AGCAGCACAACTGCTAATTATAAGGAGCGTTCGCCATTAGACACTAAACATCGACGTCAT
AATCTTACTAATGTTAATATTTTTCTATATGTCATCAAAACTTCTGAAATTCATCTTTCA
TTAACTACAGACGAATGTTATAATTTAACTATGATAAATGAACATCAAACAGTTGAAGTA
AGAATTACTGCCAATTCATTTTTTGGTGCTCGCAATGGCCTCGCAACTTTGCAGCAATTA
ATTTGGTTTGATGATGAAGATGAGTCATTGAAAATTATAGGAAGTGCAAATATTGCTGAT
TGTCCCAAATTTAACTATCGAGGTCTAATGCTTGACACGTCACGGCATTATTTTTCAGTT
GAATCAATTAAGAGAACATTAGTCGCTATGTCACATTCAAAATTAAATCGATTTCATTGG
CATCTCACTGATTCACAGAGTTTTCCATTTGTATCAAAATATTATCCTGAGCTTGCACAG
TATGGAGCTTACTCATCTAATGAGATTTATACGCGTGATGATGTTAGAGATATTGCTAAT
TTTGCTCAAGTTAGAGGAATACAAATATTAGCAGAAATCGATGCACCTGCTCATGCTGGT
AATGGTTGGCAGTGGGGTCCCGAAAAAGGTTTAGGTGAATTGAGCTTATGTGTTAATCAA
CAGCCTTGGAGTAGTTATTGTGGAGAACCACCATGCGGTCAATTAAATCCAAAAAATAAC
AACACTTTTATAATTTTACAAAGACTTTATCAAGAACTTTTAGAATTGACTGGTCCAACT
GATATGTTTCATTTAGGTGGAGATGAAGTTAATCTCGAATGCTGGGCACAATATTTTAAC
GATACAGATCTTCGTTCATTATGGTGTGATTTTATGCAGCATGCATATCATCGATTAACA
ATTGCCAATGGTGGACAATCAATTAAGATGGCAAATGTTTGGTCGAGCGGTCTTACTAGC
ATGCCATGTCTATCAAATAAGAATTTTGCTGTACAAGTTTGGGGTGGAAGTCAATGGTCT
GAAAATTATCAGTTGATTGATGCCGGATATAATTTAATTTTATCGCATGTCGATGCTTGG
TATCTCGATTGTGGTTTTGGCAATTGGAGAGCGACAGGAGAGGCTGCATGTTCGCCTTAT
AGAACGTGGCAGCAAGTTTATAAACATAAACCTTGGCAAGCTATGAGACTTAATAAACAG
CAAATGAAACAGATTCTTGGCGGAGAGGTTTGCATGTGGAGTGAGCAAGTCGATGAAAGT
TCATTGGATTCTCGTGTCTGGCCTAGAGCTGCAGCTTTAGCTGAACGCTTATGGAGTGAT
CCACAAGATGAAAATGAATATGCAATTTCCAAAGAAACATTTAATCGCTTTTCAATGTTT
CGCAATAGATTAGTGCAATTGGGTTTAAAAGCAGAACCAATATTTCCCAAATATTGCGCT
CAAAATCAGGATGAATGTGTATGA
>g8590.t1 Gene=g8590 Length=647
MAFTTSLRRLLIGLLFVMALCLMLLYFNESQSLLKPASSFLINSFDTRHNKLNPSEKAWT
YKCVNQKCVRQHYSNKNEKRVPFTSCSMLCGSYSKIWPEPTKSYIGTSANSFNLNDVQFK
IKTPFKNVENLLESAFSIFMEEIKDIFHSSSDRKTEEVSKSSTTANYKERSPLDTKHRRH
NLTNVNIFLYVIKTSEIHLSLTTDECYNLTMINEHQTVEVRITANSFFGARNGLATLQQL
IWFDDEDESLKIIGSANIADCPKFNYRGLMLDTSRHYFSVESIKRTLVAMSHSKLNRFHW
HLTDSQSFPFVSKYYPELAQYGAYSSNEIYTRDDVRDIANFAQVRGIQILAEIDAPAHAG
NGWQWGPEKGLGELSLCVNQQPWSSYCGEPPCGQLNPKNNNTFIILQRLYQELLELTGPT
DMFHLGGDEVNLECWAQYFNDTDLRSLWCDFMQHAYHRLTIANGGQSIKMANVWSSGLTS
MPCLSNKNFAVQVWGGSQWSENYQLIDAGYNLILSHVDAWYLDCGFGNWRATGEAACSPY
RTWQQVYKHKPWQAMRLNKQQMKQILGGEVCMWSEQVDESSLDSRVWPRAAALAERLWSD
PQDENEYAISKETFNRFSMFRNRLVQLGLKAEPIFPKYCAQNQDECV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 23 | g8590.t1 | CDD | cd06562 | GH20_HexA_HexB-like | 264 | 628 | 6.21744E-165 |
| 15 | g8590.t1 | Gene3D | G3DSA:3.30.379.10 | Chitobiase | 55 | 261 | 2.8E-41 |
| 16 | g8590.t1 | Gene3D | G3DSA:3.20.20.80 | Glycosidases | 262 | 647 | 1.0E-125 |
| 25 | g8590.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 154 | 175 | - |
| 3 | g8590.t1 | PANTHER | PTHR22600 | BETA-HEXOSAMINIDASE | 6 | 646 | 2.5E-259 |
| 4 | g8590.t1 | PANTHER | PTHR22600:SF3 | BETA-HEXOSAMINIDASE FDL-RELATED | 6 | 646 | 2.5E-259 |
| 22 | g8590.t1 | PIRSF | PIRSF001093 | B-hxosamndse_ab_euk_ | 11 | 647 | 2.5E-150 |
| 11 | g8590.t1 | PRINTS | PR00738 | Glycosyl hydrolase family 20 signature | 220 | 240 | 1.0E-52 |
| 5 | g8590.t1 | PRINTS | PR00738 | Glycosyl hydrolase family 20 signature | 258 | 275 | 1.0E-52 |
| 12 | g8590.t1 | PRINTS | PR00738 | Glycosyl hydrolase family 20 signature | 287 | 308 | 1.0E-52 |
| 8 | g8590.t1 | PRINTS | PR00738 | Glycosyl hydrolase family 20 signature | 341 | 358 | 1.0E-52 |
| 9 | g8590.t1 | PRINTS | PR00738 | Glycosyl hydrolase family 20 signature | 395 | 413 | 1.0E-52 |
| 7 | g8590.t1 | PRINTS | PR00738 | Glycosyl hydrolase family 20 signature | 418 | 431 | 1.0E-52 |
| 10 | g8590.t1 | PRINTS | PR00738 | Glycosyl hydrolase family 20 signature | 565 | 581 | 1.0E-52 |
| 6 | g8590.t1 | PRINTS | PR00738 | Glycosyl hydrolase family 20 signature | 582 | 599 | 1.0E-52 |
| 1 | g8590.t1 | Pfam | PF14845 | beta-acetyl hexosaminidase like | 96 | 240 | 1.5E-18 |
| 2 | g8590.t1 | Pfam | PF00728 | Glycosyl hydrolase family 20, catalytic domain | 264 | 600 | 4.1E-88 |
| 18 | g8590.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 30 | - |
| 19 | g8590.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 9 | - |
| 20 | g8590.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 10 | 25 | - |
| 21 | g8590.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 26 | 30 | - |
| 17 | g8590.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 31 | 647 | - |
| 13 | g8590.t1 | SUPERFAMILY | SSF55545 | beta-N-acetylhexosaminidase-like domain | 95 | 263 | 8.82E-19 |
| 14 | g8590.t1 | SUPERFAMILY | SSF51445 | (Trans)glycosidases | 264 | 608 | 7.16E-102 |
| 24 | g8590.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 26 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004563 | beta-N-acetylhexosaminidase activity | MF |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds | MF |
| GO:0005975 | carbohydrate metabolic process | BP |
KEGG
Orthology
- This transcript has been annotated with K20730 (beta-hexosaminidase Fdl)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
