General information for g8598.t4
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase.
Parent gene
- The parent gene g8598 is located on chr_2: 31663874..31664397 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8598 | g8598.t4 | isoform | g8598.t4 | 31663347 | 31664397 |
| chr_2 | g8598 | g8598.t4 | exon | g8598.t4.exon1 | 31663347 | 31663477 |
| chr_2 | g8598 | g8598.t4 | cds | g8598.t4.CDS1 | 31663348 | 31663477 |
| chr_2 | g8598 | g8598.t4 | exon | g8598.t4.exon2 | 31663545 | 31663623 |
| chr_2 | g8598 | g8598.t4 | cds | g8598.t4.CDS2 | 31663545 | 31663623 |
| chr_2 | g8598 | g8598.t4 | exon | g8598.t4.exon3 | 31663683 | 31663884 |
| chr_2 | g8598 | g8598.t4 | cds | g8598.t4.CDS3 | 31663683 | 31663884 |
| chr_2 | g8598 | g8598.t4 | exon | g8598.t4.exon4 | 31663945 | 31664397 |
| chr_2 | g8598 | g8598.t4 | cds | g8598.t4.CDS4 | 31663945 | 31664397 |
| chr_2 | g8598 | g8598.t4 | TSS | g8598.t4 | 31664495 | 31664495 |
| chr_2 | g8598 | g8598.t4 | TTS | g8598.t4 | NA | NA |
Sequences
>g8598.t4 Gene=g8598 Length=865
ATGAGAGCGAATAAACGTTTTCTACTTGCTCTTGGCATAATTGGTGTATGGTCGTTGCTC
ACATATCTAATTGTAATTAAAAATCACGATTATCAACAAAGTGGAAAATCAAAATTTCAC
GAGCGAATAATTTCACTAGAAAATGAAATTAGAAAAGAAACTGAAGATCGTAAAGACCTC
ATATCGCGATATCAAAATTTAATAAAAGTTCTAAGTGCACCAACAACTGCACTACCGATA
ATAAATGATAATAATATAAAAGAGAATGAAGTGGAACAGAATCAACTAAACAATAAAATA
AATTTCATTGGAAAGTATATTGATAATGATAACAATCGGCCTGTGATTCCAGTTATTGTT
ATCGCATGCAACCGCGTTTCAATTTCACGATCACTCGACCTTCTCATCAAATATCGTCCC
AATCGCGATCAATTTCCCATAATTGTGTCTCAGGATTGTGGTGATGAAGCAACACGTGAA
GTAATTTTATCATATAAGGATGAAGTGCAATTAGTACAGCAGCCAGATTTAAGTGACATT
GAAGTTCCACCAAAGGATAAAAAATTCAAAGGATATTATAAAATTTCTCGTCATTACAAT
TGGGCCTTAAATCTTGTGTTCTCTTATGGCTATGAATTTGTGATCATCGTTGAAGATGAC
CTTGATGTGGCACCCGACTTTTATGAATACTTTTTAGGAACGTTTCCACTTCTACGTGCT
GATAATACTTTATGGTGTGTTTCAGGTTGGAATGATAACGGAAAAGCTGGGTTAATTGAT
GAATCAGCTCCTGATTTGCTCTATCGTTCCGATTTCTTTCCTGGCCTCGGTTGGATGATG
AGTAAAAAATTATGGGATGAAATTG
>g8598.t4 Gene=g8598 Length=288
MRANKRFLLALGIIGVWSLLTYLIVIKNHDYQQSGKSKFHERIISLENEIRKETEDRKDL
ISRYQNLIKVLSAPTTALPIINDNNIKENEVEQNQLNNKINFIGKYIDNDNNRPVIPVIV
IACNRVSISRSLDLLIKYRPNRDQFPIIVSQDCGDEATREVILSYKDEVQLVQQPDLSDI
EVPPKDKKFKGYYKISRHYNWALNLVFSYGYEFVIIVEDDLDVAPDFYEYFLGTFPLLRA
DNTLWCVSGWNDNGKAGLIDESAPDLLYRSDFFPGLGWMMSKKLWDEI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g8598.t4 | Coils | Coil | Coil | 36 | 63 | - |
| 5 | g8598.t4 | Gene3D | G3DSA:3.90.550.10 | Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A | 114 | 288 | 7.3E-72 |
| 2 | g8598.t4 | PANTHER | PTHR10468 | PROTEIN O-LINKED-MANNOSE BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE 1/ALPHA-1,3-MANNOSYL-GLYCOPROTEIN 2-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE | 39 | 288 | 4.7E-79 |
| 1 | g8598.t4 | Pfam | PF03071 | GNT-I family | 106 | 288 | 3.6E-79 |
| 7 | g8598.t4 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 6 | - |
| 9 | g8598.t4 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 26 | - |
| 8 | g8598.t4 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 27 | 288 | - |
| 4 | g8598.t4 | SUPERFAMILY | SSF53448 | Nucleotide-diphospho-sugar transferases | 113 | 288 | 7.22E-28 |
| 3 | g8598.t4 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 26 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008375 | acetylglucosaminyltransferase activity | MF |
| GO:0006486 | protein glycosylation | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
