Gene loci information

Transcript annotation

  • This transcript has been annotated as Serine/threonine-protein kinase N.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g8641 g8641.t2 TTS g8641.t2 31996516 31996516
chr_2 g8641 g8641.t2 isoform g8641.t2 31997312 32004049
chr_2 g8641 g8641.t2 exon g8641.t2.exon1 31997312 31997455
chr_2 g8641 g8641.t2 cds g8641.t2.CDS1 31997312 31997455
chr_2 g8641 g8641.t2 exon g8641.t2.exon2 31997515 31997655
chr_2 g8641 g8641.t2 cds g8641.t2.CDS2 31997515 31997655
chr_2 g8641 g8641.t2 exon g8641.t2.exon3 31997711 31998000
chr_2 g8641 g8641.t2 cds g8641.t2.CDS3 31997711 31998000
chr_2 g8641 g8641.t2 exon g8641.t2.exon4 31998056 31998245
chr_2 g8641 g8641.t2 cds g8641.t2.CDS4 31998056 31998245
chr_2 g8641 g8641.t2 exon g8641.t2.exon5 31998315 31998833
chr_2 g8641 g8641.t2 cds g8641.t2.CDS5 31998315 31998833
chr_2 g8641 g8641.t2 exon g8641.t2.exon6 31999199 31999507
chr_2 g8641 g8641.t2 cds g8641.t2.CDS6 31999199 31999507
chr_2 g8641 g8641.t2 exon g8641.t2.exon7 31999937 32000724
chr_2 g8641 g8641.t2 cds g8641.t2.CDS7 31999937 32000724
chr_2 g8641 g8641.t2 exon g8641.t2.exon8 32000783 32001152
chr_2 g8641 g8641.t2 cds g8641.t2.CDS8 32000783 32001152
chr_2 g8641 g8641.t2 exon g8641.t2.exon9 32001697 32002658
chr_2 g8641 g8641.t2 cds g8641.t2.CDS9 32001697 32002658
chr_2 g8641 g8641.t2 exon g8641.t2.exon10 32002721 32002997
chr_2 g8641 g8641.t2 cds g8641.t2.CDS10 32002721 32002997
chr_2 g8641 g8641.t2 exon g8641.t2.exon11 32003092 32003376
chr_2 g8641 g8641.t2 cds g8641.t2.CDS11 32003092 32003376
chr_2 g8641 g8641.t2 exon g8641.t2.exon12 32003442 32003642
chr_2 g8641 g8641.t2 cds g8641.t2.CDS12 32003442 32003642
chr_2 g8641 g8641.t2 exon g8641.t2.exon13 32003816 32004049
chr_2 g8641 g8641.t2 cds g8641.t2.CDS13 32003816 32004049
chr_2 g8641 g8641.t2 TSS g8641.t2 32004474 32004474

Sequences

>g8641.t2 Gene=g8641 Length=4710
ATGGAAAAATCAAAAAATGGTGATAATGAAAGTGGCAGTAGCAGTGGTAATGAAATGACT
GCTGAATGGGTTGTCAAAGCACAGTTAAAAATTACACAACCAAAGAAAAAAGCAGCATCA
TTTACGACAAGTGATTGTGGTGCAAGATCAACTGGAAGTCCATGTAGAATTATTTATAAA
TGGACATCTGATGAGAGTAAAGCACATTTGACTGGTAAAAATCAAACAGAGAAGACATTA
AAAACTAGGTCACAGCATACACATGATGGTGCTGATTCATCCGAGATAAAACATAAACAT
GACAATAGTAAAACACTTAAAAGCACTTGTAATATAATATTGACTACAGATATTTGTCCA
AGTTCATTGAATAATGGCACGAAGATCACTCAAGATGGCTGTACAACACATACTTATACT
TTTTCGCCCGATCAGACTCAAGTATTTCGTGAAGTTAGTACACATGCACAAGAACCTTCA
ATCACTGTATCGAATGAAGAAGAAGAAAAAATATTAGTATCGAGAGATGTGAGCTGTCAG
ACAAGCGATTGCGAAGTGATACCAATCAAGAAAAAGTCTGACAGCATTAAAAATAAAAAT
GGTAGTCGATCGTCAAGTGTAGAAGGACGAAGAAAAGCATATAGTGACCATAGAAGAGCA
ACTGACTCTATAATGGCATCTTCATCAGTGAGACGAAGCGTAGAAGGAAATCGTTTAACG
CCTTCAAGAACACCAAGAAGAATGTCTCCTGCTCTTTCAAATCCTTCGGCACATTCAAAA
GAAAAGAAAAAACCACCAAGAACTGTTCATATTGATGTTTATTGCACAGGCTCAGAAGTT
GATACATCAAATAGCTCAAATGCTTCATCACCAAATATTGTCGACGATATAACTAATTTA
ATGACACAGGCGGCGACAGTTATAGATGCTGATGATATGTTATTAAAACATAGAAGAATC
GTTACTAAAAATGAATTGCCTAGAAAGCTAATGAAAGCAGAACAAAAAGCTTCCTCAACA
AAACTAGAAATAACAGAGCAAAATGACAATGAACAACAAAAGCAACAGCAGCAATTGTTT
GACTTTTCACGTGTATTGCTACAAAATAATATGAATGAAAAGGAAGAAGTCAATGAAAGT
AAACAAATGCTGTTTAAAAAATATCTTGGCGATCAACGTCAAATACAAAAATTCAATGAG
TTAAAGTCGCTAATGCATTTTCGTCGTCAGCCAAGTGATGATGCAATAAGTAGTAATTAT
CCAAATTCAAGTTATTCGACTGTTCGTGATTTTACATCAAGTTCACTTACATCAGCTGCT
GCAAATTCACCTTCAATAGTGACATTCGATGAATGTGATTGGAAAGACTTGGATAATGCA
ATTCATACAGGAAATTTTCTTACTCCAGCTGAAAGTATTCATAATGTTGATAAAAATGAT
GAACTAATGAAGATGAAAGATTTCAAACGATTATGGAGTGAACCTGGAAGTGAGCTATTG
AATCAACGTAAACTCATGGAAAACTTTTCATTGCCTTCTGGCAATTTTTCAGAGTCCGAC
AATAGTTTTCGATGTCAAAACTCTGGAAATATCAGTGATTCTAGTCCATCAACTGTTATT
AGCTATTCACACAAGAAAGCTATTAGTGAGGCAAAAATAATTAAAGAAGTCACACCCGAA
AGGAAATCGATTGAAGCGAGCACACATTCATCATCATTAAAATCGGGAAACTACTCAAAA
GATCATCTACAATTGGCACAGAAATTTGGCTCTGTGATTAAATCAATGCGCAAACCAGGT
CATCATGTCGGTCCAGTACGAAATCCATCGTGTCTATGTGAAACATGCAAACGTTGGGTG
CTTGAAAGAGATCAAGTTCAAGGACGTGAAAGAGCTTATTCTTTTGGAGAGACACCAATT
ACACGAACAACATTCTGGAAAAGAAATCATCGCTATTACGCACAAGGACGATTGATTGAG
TCATCACGAAAATTAGACCTTTTACGGTATGCATTGGAAATTCGTCGCAATGATTTACCG
CCAGATTCTGAGGCGAGTAGGCAATTAAAGACTGAACTTCAAAATGTTCAAGCAGCAAGT
CCTGTACCTGTTCAGTATACATCATTGCATCCATTTCGTGCTGGCAATCAAAGTAAAACT
TCCAACTTTTTATCACTGTCACGTTGTGCTGCTGTAACTGGTAAACTTGAAGTTCGACTT
ATGGGATGTCAGGATCTTCTTGAAGATGTACCAGGACGTTCACGAAGAGATAAAGACTCA
ATTTCAAGTCCAAGTGGAGATTTAAAAAGTTTTGTAAAGGGTGTTACTAGCCGAAGTTCA
TCAAAAAGTTATAGTGTTAAGGATGACATTTCATCAGAAATAATGGCTGTTATCAAATTA
GATAATACAACAGTTGCTCAAACATCATGGAAACCTTGTTCACAACAAGCATGGGATCAG
CGTTTTTCAATTGATCTTGATAAGTCACGTGAACTTGAAATTGGGATATATTGGCGCGAT
TGGAGATCATTATGTGCTGTTAAGTTTCTTAGATTAGAAGAGTTTATCGATGACATTCGA
CATGGAATGGCTCTTCAACTTGAACCACAAGGATTACTTTTTGCTGAAATTAAATTTCTT
AATCCAATGATTTCACGAAAACCAAAATTGCAACGTCAAAAGATGATTTTCAATAAGCAA
GCTAAAAATATTCCAAGAGCGAAGCAAATGAATATAAATATTGCGACATGGGGGCGACTA
TTAAAACGAAGTGCTCCAAGTCAACAGCAAAATAATAATGCGGTTCAAACGCCAACATCA
TCGAATCATCCATATGGAAATGGCAGTTCTTCAGTCACTGGCATTCAACCTGTTCAGTTA
ATTTTCGATGAACCGCAACAACCGCTTGATCCTGTTGAAACTGCTGGTGAAATTCCGAAT
GAAAATGTTGCCGGACTCAGTGGACAAAGGCCGTTGGGCATGCACAGCAGTGGAAGTATT
AATTCGAATGATTCAATCCCACCTCAACCACAGCCTCGAAATTATCAACCACCTCCACCT
GTTAAACAAAAGAAACCGCAAATGCCTCCAGCATTGCCACCAAAAATGGATCAAGAGGAA
TATCATCAACGTGAATCATCGTTGTCCCCACAAATGCCACAACAGCAGCAGCAACAAATA
TCACTTGATGTTGATCTCTTACGTCCTGTATTATCGATGCCGCCAGTAGTTCTTATGGGA
ATGAATGCAGAACATTCTGTCAATAGTTCAGGCAATAGCAGTCCTCGTTTGAATATTCAC
TCGATACCACCGAGTCCAATTGTTGAGATGCCTCCTTCCCCAGCGCCTTATCAATTGCCT
CTTGTTGAATTTCCCACTGAAGATGATGCTTATGAATATGATGAGCATGGTGGCGAACTT
GTCGAGATTGTAACAAATTTCGAAAACTTGAGTATTCCTTCAATGCCGTCTCAACAACCA
CAGCAACCATTCCTAATGCAACAGCCAAGTACTTCTCAATATTCTCAATCTTCTCACCAT
GTTCCTCCTCATATGATACAGCAACAGCCTGTGCCACATCACGTTGAAATGCAACAAGGT
ATCAATAATAATAACAACATCAATAATCGTCAACGACAAAATGTATCTCGTGGCTTACAA
TATCGCGATAGTGCATATGAATCGAGACGACATTCGCAATCGATGGTGATGAGTTTAGAC
AGTTTTAGATTGCTCAGTGTACTTGGTCGTGGACATTTTGGAAAAGTCATTTTGTCACAG
TATAGAAATACTGGTGAATATTTTGCCATTAAAGCATTGAAAAAAGGCGACATAATTGCA
CGTGACGAGATTGAATCACTGCTAAGTGAAAAGAGAATATTTGAAGTTGCAAATACTATG
AGACATCCATTCCTGGTTAATTTATTTGCATGCTTTCAAACTGAACAACACGTTTGTTTT
GTTATGGAATATGCAGCAGGTGGAGATTTAATGATGCACATTCATACAGATGTTTTTAGT
GAACCACGTGCAGTTTTTTATGCATCATGTGTTGTATTAGGTTTACAGTATCTTCATGAA
AATAAAATCATTTATCGTGATTTAAAATTGGATAATTTATTGCTTGATACTGAAGGATAT
GTAAAAATAGCTGATTTTGGCTTGTGTAAAGAAGGAATGGGCTTTGGTGATAGAACAGGA
ACATTCTGTGGAACACCTGAGTTTCTCGCTCCAGAAGTATTAACAGAGACTTCTTATACA
CGTTCCGTCGACTGGTGGGGTCTAGGAGTTTTAATATTTGAAATGCTTGTTGGAGAAAGT
CCATTCCCTGGTGATGATGAGGAAGAAGTTTTTGATTCAATTGTCAATGATGAAGTTCGA
TATCCACGCTTTTTGTCACTTGAAGCAATTGCTATTATGAGAAGACTTTTACGTAAAAAT
CCTGAGAGACGATTGGGAAGTTCAGAACGTGATGCAGAAGATGTTAAAAGACAAGCATTC
TTTAGAGGAATTTCTTGGGATGATTTGCTACTACGTAAAGTTAAGCCACCATTTGTTCCA
ACTATTCGTTCCATGGAAGATGTATCAAACTTTGATGAAGAATTCACATCTGAAAAACCA
CAGCTGACACCACCAAAAGAACCGAGACATTTGACAGATCAAGAACAGGATTTTTTCAAA
GATTTTTCATATATGGCTGACTGGTGCTGA

>g8641.t2 Gene=g8641 Length=1569
MEKSKNGDNESGSSSGNEMTAEWVVKAQLKITQPKKKAASFTTSDCGARSTGSPCRIIYK
WTSDESKAHLTGKNQTEKTLKTRSQHTHDGADSSEIKHKHDNSKTLKSTCNIILTTDICP
SSLNNGTKITQDGCTTHTYTFSPDQTQVFREVSTHAQEPSITVSNEEEEKILVSRDVSCQ
TSDCEVIPIKKKSDSIKNKNGSRSSSVEGRRKAYSDHRRATDSIMASSSVRRSVEGNRLT
PSRTPRRMSPALSNPSAHSKEKKKPPRTVHIDVYCTGSEVDTSNSSNASSPNIVDDITNL
MTQAATVIDADDMLLKHRRIVTKNELPRKLMKAEQKASSTKLEITEQNDNEQQKQQQQLF
DFSRVLLQNNMNEKEEVNESKQMLFKKYLGDQRQIQKFNELKSLMHFRRQPSDDAISSNY
PNSSYSTVRDFTSSSLTSAAANSPSIVTFDECDWKDLDNAIHTGNFLTPAESIHNVDKND
ELMKMKDFKRLWSEPGSELLNQRKLMENFSLPSGNFSESDNSFRCQNSGNISDSSPSTVI
SYSHKKAISEAKIIKEVTPERKSIEASTHSSSLKSGNYSKDHLQLAQKFGSVIKSMRKPG
HHVGPVRNPSCLCETCKRWVLERDQVQGRERAYSFGETPITRTTFWKRNHRYYAQGRLIE
SSRKLDLLRYALEIRRNDLPPDSEASRQLKTELQNVQAASPVPVQYTSLHPFRAGNQSKT
SNFLSLSRCAAVTGKLEVRLMGCQDLLEDVPGRSRRDKDSISSPSGDLKSFVKGVTSRSS
SKSYSVKDDISSEIMAVIKLDNTTVAQTSWKPCSQQAWDQRFSIDLDKSRELEIGIYWRD
WRSLCAVKFLRLEEFIDDIRHGMALQLEPQGLLFAEIKFLNPMISRKPKLQRQKMIFNKQ
AKNIPRAKQMNINIATWGRLLKRSAPSQQQNNNAVQTPTSSNHPYGNGSSSVTGIQPVQL
IFDEPQQPLDPVETAGEIPNENVAGLSGQRPLGMHSSGSINSNDSIPPQPQPRNYQPPPP
VKQKKPQMPPALPPKMDQEEYHQRESSLSPQMPQQQQQQISLDVDLLRPVLSMPPVVLMG
MNAEHSVNSSGNSSPRLNIHSIPPSPIVEMPPSPAPYQLPLVEFPTEDDAYEYDEHGGEL
VEIVTNFENLSIPSMPSQQPQQPFLMQQPSTSQYSQSSHHVPPHMIQQQPVPHHVEMQQG
INNNNNINNRQRQNVSRGLQYRDSAYESRRHSQSMVMSLDSFRLLSVLGRGHFGKVILSQ
YRNTGEYFAIKALKKGDIIARDEIESLLSEKRIFEVANTMRHPFLVNLFACFQTEQHVCF
VMEYAAGGDLMMHIHTDVFSEPRAVFYASCVVLGLQYLHENKIIYRDLKLDNLLLDTEGY
VKIADFGLCKEGMGFGDRTGTFCGTPEFLAPEVLTETSYTRSVDWWGLGVLIFEMLVGES
PFPGDDEEEVFDSIVNDEVRYPRFLSLEAIAIMRRLLRKNPERRLGSSERDAEDVKRQAF
FRGISWDDLLLRKVKPPFVPTIRSMEDVSNFDEEFTSEKPQLTPPKEPRHLTDQEQDFFK
DFSYMADWC

Protein features from InterProScan

Transcript Database ID Name Start End E.value
10 g8641.t2 CDD cd05589 STKc_PKN 1242 1567 0.0
9 g8641.t2 Coils Coil Coil 330 350 -
7 g8641.t2 Gene3D G3DSA:3.30.200.20 Phosphorylase Kinase; domain 1 1240 1542 6.5E-129
8 g8641.t2 Gene3D G3DSA:1.10.510.10 Transferase(Phosphotransferase) domain 1 1329 1521 6.5E-129
24 g8641.t2 MobiDBLite mobidb-lite consensus disorder prediction 1 21 -
25 g8641.t2 MobiDBLite mobidb-lite consensus disorder prediction 69 100 -
17 g8641.t2 MobiDBLite mobidb-lite consensus disorder prediction 193 219 -
19 g8641.t2 MobiDBLite mobidb-lite consensus disorder prediction 193 269 -
21 g8641.t2 MobiDBLite mobidb-lite consensus disorder prediction 221 256 -
23 g8641.t2 MobiDBLite mobidb-lite consensus disorder prediction 330 356 -
26 g8641.t2 MobiDBLite mobidb-lite consensus disorder prediction 338 356 -
18 g8641.t2 MobiDBLite mobidb-lite consensus disorder prediction 925 949 -
20 g8641.t2 MobiDBLite mobidb-lite consensus disorder prediction 1007 1060 -
22 g8641.t2 MobiDBLite mobidb-lite consensus disorder prediction 1010 1033 -
16 g8641.t2 MobiDBLite mobidb-lite consensus disorder prediction 1045 1060 -
3 g8641.t2 PANTHER PTHR24356 SERINE/THREONINE-PROTEIN KINASE 555 1569 0.0
4 g8641.t2 PANTHER PTHR24356:SF210 SERINE/THREONINE-PROTEIN KINASE N 555 1569 0.0
2 g8641.t2 Pfam PF00069 Protein kinase domain 1244 1495 5.3E-62
1 g8641.t2 Pfam PF00433 Protein kinase C terminal domain 1522 1564 1.9E-10
15 g8641.t2 ProSitePatterns PS00107 Protein kinases ATP-binding region signature. 1248 1271 -
14 g8641.t2 ProSitePatterns PS00108 Serine/Threonine protein kinases active-site signature. 1363 1375 -
28 g8641.t2 ProSiteProfiles PS50011 Protein kinase domain profile. 1242 1501 47.99
27 g8641.t2 ProSiteProfiles PS51285 AGC-kinase C-terminal domain profile. 1502 1569 17.754
11 g8641.t2 SMART SM00239 C2_3c 735 864 0.0041
13 g8641.t2 SMART SM00220 serkin_6 1242 1501 1.3E-95
12 g8641.t2 SMART SM00133 pkinase_C_6 1502 1566 2.1E-19
6 g8641.t2 SUPERFAMILY SSF49562 C2 domain (Calcium/lipid-binding domain, CaLB) 791 881 2.91E-8
5 g8641.t2 SUPERFAMILY SSF56112 Protein kinase-like (PK-like) 1239 1553 3.42E-90

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005524 ATP binding MF
GO:0004674 protein serine/threonine kinase activity MF
GO:0004672 protein kinase activity MF
GO:0006468 protein phosphorylation BP

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values