General information for g8666.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Ribosomal protein S6 kinase alpha-5.
Parent gene
- The parent gene g8666 is located on chr_2: 32159358..32163998 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8666 | g8666.t1 | TSS | g8666.t1 | 32158891 | 32158891 |
| chr_2 | g8666 | g8666.t1 | isoform | g8666.t1 | 32159358 | 32163998 |
| chr_2 | g8666 | g8666.t1 | exon | g8666.t1.exon1 | 32159358 | 32159757 |
| chr_2 | g8666 | g8666.t1 | cds | g8666.t1.CDS1 | 32159358 | 32159757 |
| chr_2 | g8666 | g8666.t1 | exon | g8666.t1.exon2 | 32159884 | 32160086 |
| chr_2 | g8666 | g8666.t1 | cds | g8666.t1.CDS2 | 32159884 | 32160086 |
| chr_2 | g8666 | g8666.t1 | exon | g8666.t1.exon3 | 32160149 | 32160349 |
| chr_2 | g8666 | g8666.t1 | cds | g8666.t1.CDS3 | 32160149 | 32160349 |
| chr_2 | g8666 | g8666.t1 | exon | g8666.t1.exon4 | 32160416 | 32160566 |
| chr_2 | g8666 | g8666.t1 | cds | g8666.t1.CDS4 | 32160416 | 32160566 |
| chr_2 | g8666 | g8666.t1 | exon | g8666.t1.exon5 | 32160628 | 32160800 |
| chr_2 | g8666 | g8666.t1 | cds | g8666.t1.CDS5 | 32160628 | 32160800 |
| chr_2 | g8666 | g8666.t1 | exon | g8666.t1.exon6 | 32160862 | 32161147 |
| chr_2 | g8666 | g8666.t1 | cds | g8666.t1.CDS6 | 32160862 | 32161147 |
| chr_2 | g8666 | g8666.t1 | exon | g8666.t1.exon7 | 32161206 | 32161333 |
| chr_2 | g8666 | g8666.t1 | cds | g8666.t1.CDS7 | 32161206 | 32161333 |
| chr_2 | g8666 | g8666.t1 | exon | g8666.t1.exon8 | 32161389 | 32163998 |
| chr_2 | g8666 | g8666.t1 | cds | g8666.t1.CDS8 | 32161389 | 32163998 |
| chr_2 | g8666 | g8666.t1 | TTS | g8666.t1 | 32164300 | 32164300 |
Sequences
>g8666.t1 Gene=g8666 Length=4152
ATGGATAAGTCAAAGAAACAATTGCAAGAAAACAAAAAAGTCATGAAAAGTAAATTAGTA
GTAGCAAATAATCATCATAATTACCAGAATGGTAGAACATCAAGTGATGACGAACACAAC
TCGTCGTGGCGAGAAACAAATAGAAATCATCATCGTGCAAAAACAGAGTCTTATGAGGAG
GAAGAAGCAGAAGAGGATATCATAGAAATTGATTCTGAAGATAGTCGAGATGCCACAAGA
AACAATAAAGAGAATCGCCACCGAGCTTATGAAAATGTAAATGAACAAAATAATCACGAT
TCTGAACGGGATAGTACTTCCAGTGATATTGTTGTCGTTCGTGATGACTTCCAAGCTTTT
TATAATGATATAGAAGAGTTATCACGAATTAATACTTTAGTGCAAATACATCCGGATGAT
AAAGTTAGCATTAAAAATTTCAAGATTCTAAAACTGTTGGGAACAGGTGCATATGGAAAA
GTATTTTCAGTTGAAAAATTAGATGGTATTGATAAAGGTGAAATCTATGCTATGAAAGTT
CTTGAAAAACATAAAGTGACACAAAAGAAAAAGACAACTGAACACACGCGAACTGAACGA
GAGGTGTTGGAAAAAGTTATCGATTGTCCTTTTCTTGCTACACTTTATTATGCATTCCAG
ACATCTGAAAAACTATATTTGATAATGGAGTTTGTTCAAGGTGGCGAATTATTCTCGCAT
CTCTACAAATCGGAAAATTTCGAAGAGAATCAAGTACGATTTTATATAGCTGAAATTGTT
GTTGCTTTAGAACAGCTTCATCAGCAAAATATTATTTATCGCGATATTAAATTGGAAAAC
ATATTGTTGGATAAGGACGGTCATATAATAATTACCGATTTTGGTTTATCGAAAGAACTC
AAAGATGGTGCTCGCACTAGTAGTTACTGTGGAACTATTGAATATATGGCACCTGAAATC
GTCAATCCAAGAAATGGTCATGATCTCAATGTCGATTGGTGGAGCGTTGGTGTTCTTATC
ATTGAATTACTCAGTGGCCAATCACCATTTTCTCGCGAAAATGAAGAAAGCAATCAACAA
ATAATTTCAGAACGCATACAACATAGTGCACCAAATATCCCTGATACTGTTGGCGATGAA
GCCAGAGATTTAATATTGAAACTTCTCGAAAAAGATCCAAAAAAGCGTTTAGGATATGTA
AATGATGCAGCAGACATTAAGAATCACAGATTTTTTCGTAACATTGACTGGAATATTTTA
AAAGAGAAAAGTTATACTGCACCTATGATTCCAAAAGTGTGCGATAAGTATGATGTCAGT
CAATTCTCTGAAGATTTTACTAATCAACCTCCTGTTGATGGTCCTGCTGATAATGGTCCT
CCACAAGGTGCAAATGCATCAAAATATTTCAGAGGATATTCTTTTGTTGCGCCCAAATTT
CGACGAAAAACTGTCACTCCTGACTTACTGTCTCTTCAAATTGCTGATAAACCTGAAATG
ATGAGACCAACAATTGAAGATGTATTTAAAGCGCAATCAAAGCGAAAATCTCCATTCTTC
ATCAAGTATCGAATTTCTCAAGAAACAGCATTGATTGGTGATGGCACATATTCTCTATGT
ATGGAATGTTTATCACTCAAAGAAAAAAAGAAGTTTGCTGTGAAAATTCTTAAAATTGAT
CATGATACATCACAAGAAATCGACGCATTAAAAAAATGTCAGGGACATCCTAATATCGTC
GAATTGGTTGAAACAATTAAAGATGAAAATTTCACATATATCGTTTTTGAACTTCTTCGT
GGTGATGAATTATTTAGTCGAATTCGTGAACATGTCTATTTTACTGAAGATATTGCACGA
ACTTACTTTCGTCAAATTGTTGATGCTGTCTCATTTATGCATAAGAAGTTTATCGTTCAT
GCTGATTTGAAACCAGAGAATATAAAATTTATGAAGAACTCTCTTGATTATGAAGAAAAA
TTGAAAATTCTCGATTTTGGTTTTGCTCATACGATTACTGATGAAGAGAAACCTCCATGT
TTTACTCTTGATTATGCAGCTCCAGAGAGTCTTATTAAAGGACCATTTTCGGAAAATCGT
GATATTTGGTCATTGGGAGTAATTCTTTATACGATGCTTATTGGAAACACTCCATTTAAG
CCACAAAATACAACTCGTGAACATGACGATCGCAACTTTCGTATCCAAATTACAGAAAAT
ATTCGCAAAGGAAAGTTCAATTGTGACAATAATCGTTGGTGTGAAATTTCAGCTGATGCA
CAAGATCTGATTTGTAAATTATTAAAAGTGAATCAAGATGAACGACTCTCAATAGAAGAA
ATCTTGGTTCATAGATGGCTTCATATCCCAGAAAATAATAACGATACTACTATGATAATT
GATAATGGTGAGATTGAACGTGCTGATTCAGATGCAACTATCATTAATGATACTGATTCA
AATGAAAATGAAGATGATAAAACAAAGATGCCTTCAGACACAGAAATTAGAAAATCTAAT
GATGATAGTAGTAGTGGCATTGTAATATCAAATGAAGGATCATCTGTTAGTGAAATTGAA
GAACATTCTGTACAAATTAGTAAAAGTGAAGAAAGAGAAATTTCTGAAATTATTATTGAA
ACTAAAGATGAAGAACCAATTTTATCAAGCACAAATAATGCTGCAGAAATTTTAAATAAA
AGTCCAAGTGAGCAATTAGAACCTGAAAATTTGAGTCTCAAACCTGCAGACATTAATAAC
AAATCTGATCAAAACAATGATGATGTTGAAATGCCTGATGCTGAAACTTTAGAAAAGCTC
TTTGATATTAAAAACTACAAAGATTTTGACACAGGCCTTACATTTAAAGATTATAAAGAA
GCAAAACGTCTAAATGATATAGCAAGACTTTGCGAAAAGAACAAGGAAGTAGAACCATTT
GAAGAGGAAGAGAATAATGAAGAAATTTGCAAAGAGGAAGACAAGAATGAAGAAATTTGC
AAAGAGGAAGACAAGAATGAAGAAATTTGCAAAGAGGAAGACAAGAATGAAGAACCTTAT
AAAGTTATAGACAAAAAAGATGAACCTTGTATAAAAGAGGACAAATTAGAAGAACCTTGT
ATAGAGAACGATAAGAAAGAAAAGCTTTATAATGAAGAAGAAAATATTAAAGAACTTTAT
CAAGAAGATGATGAATTACCGTTACAAGGCTTCAATCCATTAATTGAAGTAGCTAACGAT
GTCAGAATGCCTTTTTGTGGATTTGGAAAAGAAACGATGATGAGCAAAGAAAAATCAATT
GTAACTTCTTATGGAGATTTGTTTGATTTTATACAAAAAAAGAAAATCAAACCTGAGGCA
ATTCAAATAGTTGAAAAAGTTAAACCGAAAATCAGTGTTAAGAAAGAGAAGAAGATTAAA
AAAGAACCAACGCCTCCAACGAGACGCAGTGTTCGTCAATTACAGAGTATAAATTATCGA
ATATATCCAAAAGAAGAAAAAGTTGAAATTATTGAAGTAAAATATGAGAAACCAGTACTG
AGAACACGAAAGAATAATCAGGATGTAGCTAACAAGACTGTCGAGAATTTGCCTGAAAAG
AAAGAAAATCAGAAACAAAACCGAAAAAATATCAAAAATGAGAAATTTATTGTTATTAAA
ACAGCTTCAAGTAATACGCAAAGAGGACAAAAGCGCAAGAATCAAACAAAAGATGAAAAG
TTTGTGCCCGAAAAGAAACCAAAAGTTGAAATTAAGATGCATAAGAGTGCAGAGATGCTA
TCGCCGAAGAGAAATCGCAATAAACGAAAAGTTGAGGAAATTTCAGCTTTTTCAACTCCT
TCTTTCTCTCGGCCATCACCTATTTCTCGACATTCATTTTTCCAAACACCGCAATCGACA
GGAAATATGAATTTTTCACCGTTTTATCGAGCTAAGGCTGAACCAACTCAATTAAGCGCA
ATCAGACATCTAAACTTCCCAATTCATACAAGTCCGATTCAACAATTTGCTCAGTCTTTC
AATTACATTGACAACCGAAATAGAAATTCAATGATTATCTGTCGAAAGGACTCTTTTGCA
GGTATCAAGACTGAGATGAATTTTAATAAACCATCGATAAACGTTACTTTATTAACGCCT
TCGAGACTATAA
>g8666.t1 Gene=g8666 Length=1383
MDKSKKQLQENKKVMKSKLVVANNHHNYQNGRTSSDDEHNSSWRETNRNHHRAKTESYEE
EEAEEDIIEIDSEDSRDATRNNKENRHRAYENVNEQNNHDSERDSTSSDIVVVRDDFQAF
YNDIEELSRINTLVQIHPDDKVSIKNFKILKLLGTGAYGKVFSVEKLDGIDKGEIYAMKV
LEKHKVTQKKKTTEHTRTEREVLEKVIDCPFLATLYYAFQTSEKLYLIMEFVQGGELFSH
LYKSENFEENQVRFYIAEIVVALEQLHQQNIIYRDIKLENILLDKDGHIIITDFGLSKEL
KDGARTSSYCGTIEYMAPEIVNPRNGHDLNVDWWSVGVLIIELLSGQSPFSRENEESNQQ
IISERIQHSAPNIPDTVGDEARDLILKLLEKDPKKRLGYVNDAADIKNHRFFRNIDWNIL
KEKSYTAPMIPKVCDKYDVSQFSEDFTNQPPVDGPADNGPPQGANASKYFRGYSFVAPKF
RRKTVTPDLLSLQIADKPEMMRPTIEDVFKAQSKRKSPFFIKYRISQETALIGDGTYSLC
MECLSLKEKKKFAVKILKIDHDTSQEIDALKKCQGHPNIVELVETIKDENFTYIVFELLR
GDELFSRIREHVYFTEDIARTYFRQIVDAVSFMHKKFIVHADLKPENIKFMKNSLDYEEK
LKILDFGFAHTITDEEKPPCFTLDYAAPESLIKGPFSENRDIWSLGVILYTMLIGNTPFK
PQNTTREHDDRNFRIQITENIRKGKFNCDNNRWCEISADAQDLICKLLKVNQDERLSIEE
ILVHRWLHIPENNNDTTMIIDNGEIERADSDATIINDTDSNENEDDKTKMPSDTEIRKSN
DDSSSGIVISNEGSSVSEIEEHSVQISKSEEREISEIIIETKDEEPILSSTNNAAEILNK
SPSEQLEPENLSLKPADINNKSDQNNDDVEMPDAETLEKLFDIKNYKDFDTGLTFKDYKE
AKRLNDIARLCEKNKEVEPFEEEENNEEICKEEDKNEEICKEEDKNEEICKEEDKNEEPY
KVIDKKDEPCIKEDKLEEPCIENDKKEKLYNEEENIKELYQEDDELPLQGFNPLIEVAND
VRMPFCGFGKETMMSKEKSIVTSYGDLFDFIQKKKIKPEAIQIVEKVKPKISVKKEKKIK
KEPTPPTRRSVRQLQSINYRIYPKEEKVEIIEVKYEKPVLRTRKNNQDVANKTVENLPEK
KENQKQNRKNIKNEKFIVIKTASSNTQRGQKRKNQTKDEKFVPEKKPKVEIKMHKSAEML
SPKRNRNKRKVEEISAFSTPSFSRPSPISRHSFFQTPQSTGNMNFSPFYRAKAEPTQLSA
IRHLNFPIHTSPIQQFAQSFNYIDNRNRNSMIICRKDSFAGIKTEMNFNKPSINVTLLTP
SRL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g8666.t1 | Coils | Coil | Coil | 5 | 25 | - |
| 14 | g8666.t1 | Coils | Coil | Coil | 1042 | 1063 | - |
| 13 | g8666.t1 | Coils | Coil | Coil | 1194 | 1214 | - |
| 9 | g8666.t1 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 145 | 450 | 8.0E-118 |
| 11 | g8666.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 236 | 432 | 8.0E-118 |
| 8 | g8666.t1 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 531 | 598 | 3.5E-72 |
| 10 | g8666.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 562 | 787 | 3.5E-72 |
| 22 | g8666.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 107 | - |
| 23 | g8666.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 31 | 58 | - |
| 25 | g8666.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 73 | 107 | - |
| 27 | g8666.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 811 | 845 | - |
| 21 | g8666.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 820 | 840 | - |
| 26 | g8666.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 900 | 931 | - |
| 24 | g8666.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1220 | 1240 | - |
| 5 | g8666.t1 | PANTHER | PTHR24351 | RIBOSOMAL PROTEIN S6 KINASE | 38 | 479 | 1.6E-181 |
| 4 | g8666.t1 | PANTHER | PTHR24351 | RIBOSOMAL PROTEIN S6 KINASE | 530 | 790 | 1.6E-181 |
| 3 | g8666.t1 | Pfam | PF00069 | Protein kinase domain | 148 | 412 | 1.3E-69 |
| 1 | g8666.t1 | Pfam | PF00433 | Protein kinase C terminal domain | 437 | 475 | 3.6E-5 |
| 2 | g8666.t1 | Pfam | PF00069 | Protein kinase domain | 529 | 787 | 4.6E-63 |
| 17 | g8666.t1 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 153 | 179 | - |
| 15 | g8666.t1 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 271 | 283 | - |
| 16 | g8666.t1 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 532 | 555 | - |
| 30 | g8666.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 147 | 412 | 50.251 |
| 28 | g8666.t1 | ProSiteProfiles | PS51285 | AGC-kinase C-terminal domain profile. | 413 | 485 | 14.352 |
| 29 | g8666.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 526 | 787 | 39.891 |
| 20 | g8666.t1 | SMART | SM00220 | serkin_6 | 147 | 412 | 5.3E-101 |
| 18 | g8666.t1 | SMART | SM00133 | pkinase_C_6 | 413 | 477 | 8.7E-16 |
| 19 | g8666.t1 | SMART | SM00220 | serkin_6 | 526 | 787 | 3.3E-73 |
| 6 | g8666.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 144 | 473 | 6.12E-86 |
| 7 | g8666.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 530 | 788 | 1.62E-64 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004674 | protein serine/threonine kinase activity | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
- This transcript has been annotated with K04445 (ribosomal protein S6 kinase alpha-5)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
