General information for g8744.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as ER degradation-enhancing alpha-mannosidase-like protein 2.
Parent gene
- The parent gene g8744 is located on chr_2: 32751864..32752393 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8744 | g8744.t1 | TSS | g8744.t1 | 32749889 | 32749889 |
| chr_2 | g8744 | g8744.t1 | isoform | g8744.t1 | 32749955 | 32752393 |
| chr_2 | g8744 | g8744.t1 | exon | g8744.t1.exon1 | 32749955 | 32750040 |
| chr_2 | g8744 | g8744.t1 | cds | g8744.t1.CDS1 | 32749955 | 32750040 |
| chr_2 | g8744 | g8744.t1 | exon | g8744.t1.exon2 | 32750098 | 32750352 |
| chr_2 | g8744 | g8744.t1 | cds | g8744.t1.CDS2 | 32750098 | 32750352 |
| chr_2 | g8744 | g8744.t1 | exon | g8744.t1.exon3 | 32750409 | 32750568 |
| chr_2 | g8744 | g8744.t1 | cds | g8744.t1.CDS3 | 32750409 | 32750568 |
| chr_2 | g8744 | g8744.t1 | exon | g8744.t1.exon4 | 32750624 | 32751011 |
| chr_2 | g8744 | g8744.t1 | cds | g8744.t1.CDS4 | 32750624 | 32751011 |
| chr_2 | g8744 | g8744.t1 | exon | g8744.t1.exon5 | 32751065 | 32751190 |
| chr_2 | g8744 | g8744.t1 | cds | g8744.t1.CDS5 | 32751065 | 32751190 |
| chr_2 | g8744 | g8744.t1 | exon | g8744.t1.exon6 | 32751243 | 32752393 |
| chr_2 | g8744 | g8744.t1 | cds | g8744.t1.CDS6 | 32751243 | 32752393 |
| chr_2 | g8744 | g8744.t1 | TTS | g8744.t1 | 32752425 | 32752425 |
Sequences
>g8744.t1 Gene=g8744 Length=2166
ATGAAGTTATTCTTAATTTTAACATTAGCATTCAATTTAACAAATTCACTTCGACAATAT
AAGAAGGAAGATGTTTTAAGATTAAGAGAACAAGCTAGAAAAATGTTTTATCATGGATAT
GACAACTATCTTAAATATGCATCAAAATATGATGAATTAAAACCTCTGAGTTGTACAGGA
GTAAATACATGGAGCAGTTCTTCTTTAACATTAATTGACTCTTTAGACACATTAATTGTA
ATGGGCAATTATTCTGAATTTAAAAGAGTCTCATCATATATAGTTGAAAATTTTGATTTT
GATAAAGACATTAATGTGTCAGTTTTTGAAGTAAATATTAGGATTGTTGGCGGTCTATTA
TCAGCACACTTGCTTTCACATAAAGCAGGAATAAATTATGTAGGATTTCCTTGCAATGGA
CCACTACTTCAACTTGCGGAAAAAGTTGCAAGAAAATTGTTGCCAGCATTCGATACAAAG
ACTGGAATGCCTTATGGCACGGTAAATTTAAAATATGGAGTACCTGAGAGTGAAACTAGT
GTTACTTGTGTAGCAGCAATTGGAACATTTGTCGTAGAATTTGGAACTTTGTCACGTTTG
ACAGGTGATCCAATTTTTGAGGAAGTTGCTGTAAATGCACTTTATTCACTCTTCAATCAT
CGCTCACAAATTGGCTTACTTGGAAATCATATTGATGTTCAAACTGGAGTATGGACAGCA
CACGATAGTGGCATTGGTGCTGGAGTTGATTCATATTTTGAATACCTTGTTAAAGGAGCA
ATTTTACTAAATCGTCCTGAACTTATGGAAATGTTCAATGAAGCACGCAAATCAGTAGAA
AAATATTTAAATAAAGATGATTGGCAAGTGTGGGTCAGCATGAGTAAAGGACAAATGACA
TTACCGGTATTTCAAAGTCTTCAAGCATTCTGGGCAGGATTACTTTCACTAATTGGTGAT
ACTAATAATGCAATGAAAATTCTCTATAATATGCACAGTGTATGGAAACAATATGGTTTT
CTTCCTGAAATGTTTAATATTCCAAATGGTGAACCAAGTACAAATCGAGAATCTTATCCA
TTACGACCTGAATTAATTGAAAGTGTTATGTATTTATATCGTGCAACTGGTGATCCTTAT
TTGCTAGAAATTGGTGAAGACATTTTAACATCAATAGAACATAGTGCTAAAACAAGCTGT
GGTTATGCTACCATAAAAAATGTTAAAGATCATCGTAAACAAGACACTATGGAATCATTT
TTCCTTGCTGAAACCACAAAATATCTTTATCTCCTTTTTGATCCTGACAACTTTTTAAAT
AGTGATGGTTCATATGGTACAATTATTGATACAAAAAATGGTGAATGTGTGATTGAATCT
GCTTATATTTTTAATACTGAAGCACATCCGATAGATTTAGGAGCATTAAAATGTTGCTAT
GATATGCCACGAGAATCTTTAGTTAAAGGTTTTGACAAGAAAAAGTTTCTTGGTGATTTG
GCAATTGAAAAGGTTGATGATAATGAAAATCAAATGAAAATTCTTGATGGAATTGATGAA
AATTCCAATGCGAATGAAGATTTCAAAAAGCAATTATTAGAAGAAATTTTAAATGCACTT
TCAAATGCTAACAAAAAAATGAAAGAAAATTCTGAAGTTCAAAAGCGATTTGTTGAAATG
ACTAGTCGTGAAAAGCAACAAAATTTTGAAGAAAGCAATAAAGTTAAAATTAAAGAAGAA
GAAAGTGCAATAACATCAAATATTGATGACAATGAAAAATTAGAAACTGTTCGTTTATCT
TCTAATGCTCTCGACTCAGACGATGCAAATCAATCTAAATATGAAGAAGTCAAGAATGAA
GAAATTCTAAAAAATGAAAATTTACAAAGTATACAAAATGAACAGAAAATTGTAAAGAAA
GTTTCTGAAAAAGTTAATCTTAATAATAACAATAATTCTATAATAACTGATTTTGTAAAA
AATATTCTAAAGTCTGCAACAACTAAACAAAAGAAGAAATTTGATGCTCAAAATTTGTTG
TCAAAAATTAAACTCGAGCAATTTGGATTTACCAGAAATTCTTCATGGACTGAGAAATAT
GAATTACTTTCTTGCAAAGCTCAGGCTTATGGAAATAGAATATCTGCAATGGGCGAATTT
TATTAA
>g8744.t1 Gene=g8744 Length=721
MKLFLILTLAFNLTNSLRQYKKEDVLRLREQARKMFYHGYDNYLKYASKYDELKPLSCTG
VNTWSSSSLTLIDSLDTLIVMGNYSEFKRVSSYIVENFDFDKDINVSVFEVNIRIVGGLL
SAHLLSHKAGINYVGFPCNGPLLQLAEKVARKLLPAFDTKTGMPYGTVNLKYGVPESETS
VTCVAAIGTFVVEFGTLSRLTGDPIFEEVAVNALYSLFNHRSQIGLLGNHIDVQTGVWTA
HDSGIGAGVDSYFEYLVKGAILLNRPELMEMFNEARKSVEKYLNKDDWQVWVSMSKGQMT
LPVFQSLQAFWAGLLSLIGDTNNAMKILYNMHSVWKQYGFLPEMFNIPNGEPSTNRESYP
LRPELIESVMYLYRATGDPYLLEIGEDILTSIEHSAKTSCGYATIKNVKDHRKQDTMESF
FLAETTKYLYLLFDPDNFLNSDGSYGTIIDTKNGECVIESAYIFNTEAHPIDLGALKCCY
DMPRESLVKGFDKKKFLGDLAIEKVDDNENQMKILDGIDENSNANEDFKKQLLEEILNAL
SNANKKMKENSEVQKRFVEMTSREKQQNFEESNKVKIKEEESAITSNIDDNEKLETVRLS
SNALDSDDANQSKYEEVKNEEILKNENLQSIQNEQKIVKKVSEKVNLNNNNNSIITDFVK
NILKSATTKQKKKFDAQNLLSKIKLEQFGFTRNSSWTEKYELLSCKAQAYGNRISAMGEF
Y
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 16 | g8744.t1 | Coils | Coil | Coil | 529 | 553 | - |
| 15 | g8744.t1 | Coils | Coil | Coil | 614 | 634 | - |
| 14 | g8744.t1 | Gene3D | G3DSA:1.50.10.10 | - | 17 | 480 | 6.0E-151 |
| 2 | g8744.t1 | PANTHER | PTHR45679 | ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2 | 15 | 615 | 1.2E-229 |
| 3 | g8744.t1 | PANTHER | PTHR45679:SF6 | ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2 | 15 | 615 | 1.2E-229 |
| 8 | g8744.t1 | PRINTS | PR00747 | Glycosyl hydrolase family 47 signature | 35 | 55 | 3.0E-63 |
| 10 | g8744.t1 | PRINTS | PR00747 | Glycosyl hydrolase family 47 signature | 68 | 82 | 3.0E-63 |
| 5 | g8744.t1 | PRINTS | PR00747 | Glycosyl hydrolase family 47 signature | 105 | 123 | 3.0E-63 |
| 7 | g8744.t1 | PRINTS | PR00747 | Glycosyl hydrolase family 47 signature | 155 | 174 | 3.0E-63 |
| 4 | g8744.t1 | PRINTS | PR00747 | Glycosyl hydrolase family 47 signature | 246 | 263 | 3.0E-63 |
| 11 | g8744.t1 | PRINTS | PR00747 | Glycosyl hydrolase family 47 signature | 303 | 319 | 3.0E-63 |
| 6 | g8744.t1 | PRINTS | PR00747 | Glycosyl hydrolase family 47 signature | 360 | 384 | 3.0E-63 |
| 9 | g8744.t1 | PRINTS | PR00747 | Glycosyl hydrolase family 47 signature | 419 | 439 | 3.0E-63 |
| 1 | g8744.t1 | Pfam | PF01532 | Glycosyl hydrolase family 47 | 35 | 471 | 7.0E-116 |
| 18 | g8744.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 16 | - |
| 19 | g8744.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 20 | g8744.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 11 | - |
| 21 | g8744.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 12 | 16 | - |
| 17 | g8744.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 17 | 721 | - |
| 12 | g8744.t1 | SUPERFAMILY | SSF48225 | Seven-hairpin glycosidases | 23 | 472 | 4.58E-144 |
| 13 | g8744.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 16 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0005509 | calcium ion binding | MF |
| GO:0004571 | mannosyl-oligosaccharide 1,2-alpha-mannosidase activity | MF |
| GO:0005975 | carbohydrate metabolic process | BP |
KEGG
Orthology
- This transcript has been annotated with K10085 (ER degradation enhancer, mannosidase alpha-like 2)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
