General information for g8746.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Serine protease 2.
Parent gene
- The parent gene g8746 is located on chr_2: 32757910..32760752 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8746 | g8746.t1 | isoform | g8746.t1 | 32756973 | 32757910 |
| chr_2 | g8746 | g8746.t1 | exon | g8746.t1.exon1 | 32756973 | 32757261 |
| chr_2 | g8746 | g8746.t1 | cds | g8746.t1.CDS1 | 32756973 | 32757261 |
| chr_2 | g8746 | g8746.t1 | exon | g8746.t1.exon2 | 32757315 | 32757910 |
| chr_2 | g8746 | g8746.t1 | cds | g8746.t1.CDS2 | 32757315 | 32757910 |
| chr_2 | g8746 | g8746.t1 | TSS | g8746.t1 | NA | NA |
| chr_2 | g8746 | g8746.t1 | TTS | g8746.t1 | NA | NA |
Sequences
>g8746.t1 Gene=g8746 Length=885
ATGCATTCATTAGGAATCACTTTATTACTTTTCAATTTATTTTTCTTCACATCAAGTTTT
TCAAATTTTAATATAAAAAATAAAGAAATTTTAGCTCAAAAACCAAAATTTGAAAGTAAA
AAATTAATTGAAAGTTTTAGAAAAAAAGGAAGAATTACAAATGGCTTTATTGCCAATAAT
GGTTCAAATTTTCTTCCATTTTTTACTTTACTTTATGTAAGAAAAGTTACAAATATTTAC
AACATGTGTGCTGGTTCAATCATTTCTGAAAAATTTCTTATTAGTGCTGGACATTGTTTT
GAAAGTTCAGCTGAAGTTTTCATCTTAGCTGGTTTTAACAACAGTAATTTGGAATCTTAT
ACATTTTTCGATCAAGTCGCATCAAAAAATGTCATTGTGCATCCATCTTATAATTCTACA
ACAATTTCCAATGATATTGCACTCATAAAATTTTCACAAAATATCATTTTTAATCTTGTT
TTACAAAAAATTAAACTTCCATTAACCAATTCAAATTTTATGAACAAATCAGGAATTGTA
AGTGGTTTTGGGATTTTTAGTGATGTAAATGCAACAACTTCGAATGTTCTTCGTTATACT
TTTGTGACTGTCATCAATGGAAGCACTTGTAATTTGTCTTATGGTTCAAATTTCTTTAGC
AATGATAGGCAACTTTGTATTGATACTAAAAGTGGTCATTCTTCAACATGCAGTGGTGAT
AGTGGAGCAAGTTTGGGAATTTTTGAGAATAATATTTTGACTTTAGCTGGAATTGTTAGT
TATGGAAGCACTCAAGGTTGTGAATTGGGAAATCCAGAGGTTTATACAAAAGTCAGTTCT
TATCTGCAATGGATTGGAAATAAAACTGGAATTGTAGTTTTATAA
>g8746.t1 Gene=g8746 Length=294
MHSLGITLLLFNLFFFTSSFSNFNIKNKEILAQKPKFESKKLIESFRKKGRITNGFIANN
GSNFLPFFTLLYVRKVTNIYNMCAGSIISEKFLISAGHCFESSAEVFILAGFNNSNLESY
TFFDQVASKNVIVHPSYNSTTISNDIALIKFSQNIIFNLVLQKIKLPLTNSNFMNKSGIV
SGFGIFSDVNATTSNVLRYTFVTVINGSTCNLSYGSNFFSNDRQLCIDTKSGHSSTCSGD
SGASLGIFENNILTLAGIVSYGSTQGCELGNPEVYTKVSSYLQWIGNKTGIVVL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g8746.t1 | CDD | cd00190 | Tryp_SPc | 52 | 285 | 1.01108E-53 |
| 9 | g8746.t1 | Gene3D | G3DSA:2.40.10.10 | - | 45 | 290 | 3.5E-51 |
| 2 | g8746.t1 | PANTHER | PTHR24250:SF56 | SERINE PROTEASE P96 | 43 | 288 | 8.5E-54 |
| 3 | g8746.t1 | PANTHER | PTHR24250 | CHYMOTRYPSIN-RELATED | 43 | 288 | 8.5E-54 |
| 6 | g8746.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 84 | 99 | 3.3E-9 |
| 4 | g8746.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 141 | 155 | 3.3E-9 |
| 5 | g8746.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 234 | 246 | 3.3E-9 |
| 1 | g8746.t1 | Pfam | PF00089 | Trypsin | 57 | 285 | 7.6E-39 |
| 11 | g8746.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 21 | - |
| 12 | g8746.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 13 | g8746.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 16 | - |
| 14 | g8746.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 17 | 21 | - |
| 10 | g8746.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 22 | 294 | - |
| 17 | g8746.t1 | ProSitePatterns | PS00134 | Serine proteases, trypsin family, histidine active site. | 94 | 99 | - |
| 18 | g8746.t1 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 52 | 290 | 22.604 |
| 16 | g8746.t1 | SMART | SM00020 | trypsin_2 | 51 | 285 | 4.6E-43 |
| 7 | g8746.t1 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 50 | 290 | 1.16E-58 |
| 8 | g8746.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 19 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed