Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative 2-hydroxyacylsphingosine 1-beta-galactosyltransferase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g877 g877.t13 TSS g877.t13 6618107 6618107
chr_3 g877 g877.t13 isoform g877.t13 6618133 6620603
chr_3 g877 g877.t13 exon g877.t13.exon1 6618133 6618367
chr_3 g877 g877.t13 cds g877.t13.CDS1 6618133 6618367
chr_3 g877 g877.t13 exon g877.t13.exon2 6618433 6618718
chr_3 g877 g877.t13 cds g877.t13.CDS2 6618433 6618718
chr_3 g877 g877.t13 exon g877.t13.exon3 6619039 6619626
chr_3 g877 g877.t13 cds g877.t13.CDS3 6619039 6619626
chr_3 g877 g877.t13 exon g877.t13.exon4 6619689 6620603
chr_3 g877 g877.t13 cds g877.t13.CDS4 6619689 6619698
chr_3 g877 g877.t13 TTS g877.t13 6620992 6620992

Sequences

>g877.t13 Gene=g877 Length=2024
ATGAAGCTCTCTTTAGCATTAATTGTTGTTTTTATCAATGGAATATTTGGTTTTAAAGTG
TTGGGAATTTTGCCTTTTGGCAGCAAGTCACATTTTGCAATTGGACATGCAATTTTGAAA
AGTTTAGCAGAAGCTGGACATAATGTAACGTCGATTTCACCATATCCATTAAAAGAACCT
ATGGAAAATTACAAAGATATCAGCACAGAAGATTACGTTGAAGTATTTTTTAAAAATAAT
GCTGTGAATATGTTTGCTTTTGAAAATACTCCTATCGTCAATAAAATTATGGAATTAATA
TTTATTTATTGGATTAATCGAAATGGCAATGAAGTTGTGAAATATCATGCAGTTCATCCT
AAAGTTATTGAATTTATGAACACTTATGAGAAGTTTGATATTTGCTTTATGGAAATTTTT
AATTATGATGCATTACTTGGAATTGCCGAACATGTTGGTTGCAAAGTTATTTCGTATACC
ACAACAGCTGTTGTTAAATGGGCTGATGATATGACTGGAAACGTTTCACCACCATCATAT
GTACCAAGACCGTATGTTCAATATTCAGATAAAATGTCATTCAGAGAACGACTTATGAAT
ACATTTTACGCTCACATTGAAGACATAGTTTATGAATTCATCATAAAACCAAACCAAAGA
AGACTTTATGAGAAATATTTCCCAAATGCAACTAAAACATTTGACGAGATGTACAAAGAT
ACTTCAATGATTTTCATGAACACTCACATTTCACTGACAGCACCTCGTCCGTATTTACCA
AGTTTTGTAGAAATTGGAGGAATTCATATTCAACCTGTAAAAAAGTTGCCAAAAGTATTT
CAAAATTTCTTAGATTCAGCGACTGATGGTGTGATTTTATTTTCAATGGGTTCAGTAATT
CGATCATACCAATGGCCAGTTGAGAAACGTGAAGCATTCGTTAAAGTTTTTGGCAAATTG
AAGCAAAAAGTACTTTGGAAATATGAAAATGACACATTACCAAATAAACCTGATAATGTT
ATGATTAGTTCATGGTTGCCACAAAGAGACATTTTGGCTCATCCTAATGTAAAAGCTTAT
ATCTGTCATGGCGGACTATTAGGAACAACCAGTTTATGAAGGAGTCCCTGTGCTTGGTAT
ACCAATATTCGGAGACCAAAAGACAATCATGGCTAAAGCAGTTACTCGTGGCTATGGATT
GCAAGTTTATTTCAAAGATGTTTCTGAAGAAACAATTGGTTCTGCCTTGGATGAATTATT
GAACAATCCAATGTACAAAGAAAATGCAAAAATTATTTCAACACGCTTTAAAGATCGACC
AATGACACCACAACAATCAGTAGTTTATTGGACTGAATATGTTCATAGACACGGTGGAGC
ACCACACTTGAAAGCTGCTGGAAATGATTTGGATTTTATCGCATTTCATATGATTGATGT
TTATGCTGTTTTGTTTTTTGGTGGAATTTCTTTCATTTTCATTAATTATTTGATTATTAA
GTGGATTTTTATGAAATTTTCTGGAAAACCAAAGAAGACAGAAAAGAAAAATGAGAAGAA
GCTTAAAAAGAAATAAACAAGAAATAATAGAATATTTCAACCATTAGCTGTGACCAGTAA
AATTTCAATCAGGGAACTGAGTTCTGTTCATATCGAATGAAAAATATTTTTGAACAAATT
TTAAAAGAAATCTCTGAATTCATTTATTTATTTACATTTAAGATTATGGCTTAATATTTT
GCAACAATTAAAAACTTAGTTCATTAAAAAGTGTTCCTTACATTTTTCATTATTTTATAT
TTTATTATTTCTTGAAAAATTATTTAAGTTCAAAATAATTTCCTTAAAATTCCATGTAAA
GCTATAAACAGTGATATTTTCTTTTACTTTTGCTCAATTAAATTTTATTATTTATTTTCT
AGCTATATTTTCATTTTAATTAAAGTTCATTTTCCTAAAAAAATTTTTGGCAAAAGAAAT
CATGAAATTTTCAAAAAACATGAAAGAATGAGAGTGATTGTAAA

>g877.t13 Gene=g877 Length=372
MKLSLALIVVFINGIFGFKVLGILPFGSKSHFAIGHAILKSLAEAGHNVTSISPYPLKEP
MENYKDISTEDYVEVFFKNNAVNMFAFENTPIVNKIMELIFIYWINRNGNEVVKYHAVHP
KVIEFMNTYEKFDICFMEIFNYDALLGIAEHVGCKVISYTTTAVVKWADDMTGNVSPPSY
VPRPYVQYSDKMSFRERLMNTFYAHIEDIVYEFIIKPNQRRLYEKYFPNATKTFDEMYKD
TSMIFMNTHISLTAPRPYLPSFVEIGGIHIQPVKKLPKVFQNFLDSATDGVILFSMGSVI
RSYQWPVEKREAFVKVFGKLKQKVLWKYENDTLPNKPDNVMISSWLPQRDILAHPNVKAY
ICHGGLLGTTSL

Protein features from InterProScan

Transcript Database ID Name Start End E.value
12 g877.t13 CDD cd03784 GT1_Gtf-like 19 370 1.78774E-38
6 g877.t13 Gene3D G3DSA:3.40.50.2000 Glycogen Phosphorylase B; 271 372 8.6E-23
2 g877.t13 PANTHER PTHR48043 EG:EG0003.4 PROTEIN-RELATED 16 370 3.3E-94
3 g877.t13 PANTHER PTHR48043:SF114 DOROTHY, ISOFORM A-RELATED 16 370 3.3E-94
1 g877.t13 Pfam PF00201 UDP-glucoronosyl and UDP-glucosyl transferase 30 368 3.0E-43
8 g877.t13 Phobius SIGNAL_PEPTIDE Signal peptide region 1 17 -
9 g877.t13 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 2 -
10 g877.t13 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 3 12 -
11 g877.t13 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 13 17 -
7 g877.t13 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 18 372 -
5 g877.t13 SUPERFAMILY SSF53756 UDP-Glycosyltransferase/glycogen phosphorylase 20 369 6.73E-70
4 g877.t13 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 5 27 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0008194 UDP-glycosyltransferase activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values