General information for g877.t33
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as 2-hydroxyacylsphingosine 1-beta-galactosyltransferase.
Parent gene
- The parent gene g877 is located on chr_3: 6619998..6629641 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g877 | g877.t33 | TSS | g877.t33 | 6618107 | 6618107 |
| chr_3 | g877 | g877.t33 | isoform | g877.t33 | 6618433 | 6620155 |
| chr_3 | g877 | g877.t33 | exon | g877.t33.exon1 | 6618433 | 6618718 |
| chr_3 | g877 | g877.t33 | cds | g877.t33.CDS1 | 6618447 | 6618718 |
| chr_3 | g877 | g877.t33 | exon | g877.t33.exon2 | 6619039 | 6619626 |
| chr_3 | g877 | g877.t33 | cds | g877.t33.CDS2 | 6619039 | 6619626 |
| chr_3 | g877 | g877.t33 | exon | g877.t33.exon3 | 6619684 | 6620155 |
| chr_3 | g877 | g877.t33 | cds | g877.t33.CDS3 | 6619684 | 6620155 |
| chr_3 | g877 | g877.t33 | TTS | g877.t33 | 6620992 | 6620992 |
Sequences
>g877.t33 Gene=g877 Length=1346
ATAATGCTGTGAATATGTTTGCTTTTGAAAATACTCCTATCGTCAATAAAATTATGGAAT
TAATATTTATTTATTGGATTAATCGAAATGGCAATGAAGTTGTGAAATATCATGCAGTTC
ATCCTAAAGTTATTGAATTTATGAACACTTATGAGAAGTTTGATATTTGCTTTATGGAAA
TTTTTAATTATGATGCATTACTTGGAATTGCCGAACATGTTGGTTGCAAAGTTATTTCGT
ATACCACAACAGCTGTTGTTAAATGGGCTGATGATATGACTGGAAACGTTTCACCACCAT
CATATGTACCAAGACCGTATGTTCAATATTCAGATAAAATGTCATTCAGAGAACGACTTA
TGAATACATTTTACGCTCACATTGAAGACATAGTTTATGAATTCATCATAAAACCAAACC
AAAGAAGACTTTATGAGAAATATTTCCCAAATGCAACTAAAACATTTGACGAGATGTACA
AAGATACTTCAATGATTTTCATGAACACTCACATTTCACTGACAGCACCTCGTCCGTATT
TACCAAGTTTTGTAGAAATTGGAGGAATTCATATTCAACCTGTAAAAAAGTTGCCAAAAG
TATTTCAAAATTTCTTAGATTCAGCGACTGATGGTGTGATTTTATTTTCAATGGGTTCAG
TAATTCGATCATACCAATGGCCAGTTGAGAAACGTGAAGCATTCGTTAAAGTTTTTGGCA
AATTGAAGCAAAAAGTACTTTGGAAATATGAAAATGACACATTACCAAATAAACCTGATA
ATGTTATGATTAGTTCATGGTTGCCACAAAGAGACATTTTGGCTCATCCTAATGTAAAAG
CTTATATCTGTCATGGCGGACTATTAGGAACAACTGAAGCAGTTTATGAAGGAGTCCCTG
TGCTTGGTATACCAATATTCGGAGACCAAAAGACAATCATGGCTAAAGCAGTTACTCGTG
GCTATGGATTGCAAGTTTATTTCAAAGATGTTTCTGAAGAAACAATTGGTTCTGCCTTGG
ATGAATTATTGAACAATCCAATGTACAAAGAAAATGCAAAAATTATTTCAACACGCTTTA
AAGATCGACCAATGACACCACAACAATCAGTAGTTTATTGGACTGAATATGTTCATAGAC
ACGGTGGAGCACCACACTTGAAAGCTGCTGGAAATGATTTGGATTTTATCGCATTTCATA
TGATTGATGTTTATGCTGTTTTGTTTTTTGGTGGAATTTCTTTCATTTTCATTAATTATT
TGATTATTAAGTGGATTTTTATGAAATTTTCTGGAAAACCAAAGAAGACAGAAAAGAAAA
ATGAGAAGAAGCTTAAAAAGAAATAA
>g877.t33 Gene=g877 Length=443
MFAFENTPIVNKIMELIFIYWINRNGNEVVKYHAVHPKVIEFMNTYEKFDICFMEIFNYD
ALLGIAEHVGCKVISYTTTAVVKWADDMTGNVSPPSYVPRPYVQYSDKMSFRERLMNTFY
AHIEDIVYEFIIKPNQRRLYEKYFPNATKTFDEMYKDTSMIFMNTHISLTAPRPYLPSFV
EIGGIHIQPVKKLPKVFQNFLDSATDGVILFSMGSVIRSYQWPVEKREAFVKVFGKLKQK
VLWKYENDTLPNKPDNVMISSWLPQRDILAHPNVKAYICHGGLLGTTEAVYEGVPVLGIP
IFGDQKTIMAKAVTRGYGLQVYFKDVSEETIGSALDELLNNPMYKENAKIISTRFKDRPM
TPQQSVVYWTEYVHRHGGAPHLKAAGNDLDFIAFHMIDVYAVLFFGGISFIFINYLIIKW
IFMKFSGKPKKTEKKNEKKLKKK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g877.t33 | CDD | cd03784 | GT1_Gtf-like | 37 | 369 | 4.27358E-54 |
| 6 | g877.t33 | Coils | Coil | Coil | 430 | 443 | - |
| 5 | g877.t33 | Gene3D | G3DSA:3.40.50.2000 | Glycogen Phosphorylase B; | 188 | 357 | 4.4E-44 |
| 2 | g877.t33 | PANTHER | PTHR48043 | EG:EG0003.4 PROTEIN-RELATED | 8 | 408 | 9.2E-119 |
| 3 | g877.t33 | PANTHER | PTHR48043:SF114 | DOROTHY, ISOFORM A-RELATED | 8 | 408 | 9.2E-119 |
| 1 | g877.t33 | Pfam | PF00201 | UDP-glucoronosyl and UDP-glucosyl transferase | 46 | 431 | 3.5E-83 |
| 8 | g877.t33 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 398 | - |
| 9 | g877.t33 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 399 | 422 | - |
| 7 | g877.t33 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 423 | 443 | - |
| 12 | g877.t33 | ProSitePatterns | PS00375 | UDP-glycosyltransferases signature. | 262 | 305 | - |
| 4 | g877.t33 | SUPERFAMILY | SSF53756 | UDP-Glycosyltransferase/glycogen phosphorylase | 32 | 382 | 2.13E-93 |
| 11 | g877.t33 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 401 | 423 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008194 | UDP-glycosyltransferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K04628 (ceramide galactosyltransferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
