General information for g8960.t22
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Arginine kinase.
Parent gene
- The parent gene g8960 is located on chr_2: 34214230..34214913 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8960 | g8960.t22 | isoform | g8960.t22 | 34206316 | 34214613 |
| chr_2 | g8960 | g8960.t22 | exon | g8960.t22.exon1 | 34206316 | 34206429 |
| chr_2 | g8960 | g8960.t22 | TSS | g8960.t22 | 34206336 | 34206336 |
| chr_2 | g8960 | g8960.t22 | exon | g8960.t22.exon2 | 34213056 | 34213280 |
| chr_2 | g8960 | g8960.t22 | exon | g8960.t22.exon3 | 34213336 | 34213874 |
| chr_2 | g8960 | g8960.t22 | cds | g8960.t22.CDS1 | 34213464 | 34213874 |
| chr_2 | g8960 | g8960.t22 | exon | g8960.t22.exon4 | 34213936 | 34214157 |
| chr_2 | g8960 | g8960.t22 | cds | g8960.t22.CDS2 | 34213936 | 34214157 |
| chr_2 | g8960 | g8960.t22 | exon | g8960.t22.exon5 | 34214230 | 34214613 |
| chr_2 | g8960 | g8960.t22 | cds | g8960.t22.CDS3 | 34214230 | 34214277 |
| chr_2 | g8960 | g8960.t22 | TTS | g8960.t22 | 34214625 | 34214625 |
Sequences
>g8960.t22 Gene=g8960 Length=1484
GCACAGTTCTCGAGCGATTCTGTTTCAAAAACAGTTTGTGAAAAGTCACATTCAGTTGAA
TCTTCTTCAGTGCAAATTCACTCAAAGGTGAGAAATAAATAAATTTATTGAATAGCTTTG
CCAAGCTCCAAGCCTCTGACTCGAAATCATTGCTTAAAAAGTATTTGACCAAGGAAGTCT
TTGATAATCTTAAGAACAAGAAGACCCCAACATTTGGTTCAACTTTGCTTGATGTCATCC
AATCTGGTTTAGAAAACCACGACTCTGGCGTCGGAATTTACGCACCAGATGCCGAGGCTT
ACACAACTTTCGCTGAATTGTTTGACCCAATCATTGAAGACTACCATGGTGGATTCAAGA
AGACCGACAAGCATCCAGCAAAGGACTTTGGTGATGTAAATGCATTTGGAGATTTGGATC
CAACTGGTGAATTCGTTGTCTCAACTCGTGTCCGTTGCGGTCGCTCAATGGAAGGATATC
CATTCAATCCATGCTTGACTGAAGCACAATACAAAGAAATGGAACAAAAGGTTTCAACCA
CATTGTCAGGCTTGGAAGGTGAATTGAAGGGCAAATTCTACCCATTGACTGGCATGGACA
AAGCTACTCAACAACAACTCATCGATGACCATTTCTTGTTCAAAGAAGGTGATCGCTTCT
TGCAAGCTGCCAATGCTTGCCGCTATTGGCCATCAGGTCGCGGTATCTACCACAATGATG
CCAAGACTTTCCTTGTCTGGTGCAATGAAGAAGATCACTTGCGTATCATTTCAATGCAAA
TGGGTGGCAATCTTGGTGAAGTTTTCCGTCGTTTGACAACTGCCGTTAATGACATTGAGA
AGCGTATTCCATTCAGCCACAACGACCGCTTGGGATTCCTCACATTCTGCCCAACCAACT
TGGGAACAACCATCCGTGCATCAGTTCACATTAAGGTTCCAAAATTGGCTGCCAACAAAG
CTCGTCTTGAGGAAGTTGCTTCCAAATACAATTTGCAAGTTCGTGGCACACGCGGAGAAC
ATACAGAGGCTGAAGGTGGCATTTATGATATTTCAAACAAGAGACGTATGGGCTTGACTG
AATTCCAAGCTGTCAAGGAGATGTACGATGGAATTACTGAAATCATCAAGATTGAAAAGT
CATTGTAAAGTGTGTAAAAGCAAAAACAAAACAACCCAACTTAAAATTGTCATCTATTTT
CAAAATTATACATTAATATAAAAAACACATATACACACATGAATTAACAACAAAACTCAA
TCGTCATTTGCAATGGCGATCAAAGATTTGGAAATCTTTCATCAGTGATCTTTATTCTTG
TTTAATTTAATTGATAAAAAAATGTGTCCTATTCTCTCCATCTCAGTATAAGTTTGATGT
GATTGATCACAAGCATTAAGAATATTTTGGAATAAAATGTGAATTTAACATCTATTACCA
CATACAGCACATGCAAACAAGAAGTTAATTAAATAAAAACCGTA
>g8960.t22 Gene=g8960 Length=226
MEGYPFNPCLTEAQYKEMEQKVSTTLSGLEGELKGKFYPLTGMDKATQQQLIDDHFLFKE
GDRFLQAANACRYWPSGRGIYHNDAKTFLVWCNEEDHLRIISMQMGGNLGEVFRRLTTAV
NDIEKRIPFSHNDRLGFLTFCPTNLGTTIRASVHIKVPKLAANKARLEEVASKYNLQVRG
TRGEHTEAEGGIYDISNKRRMGLTEFQAVKEMYDGITEIIKIEKSL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g8960.t22 | Gene3D | G3DSA:3.30.590.10 | Glutamine synthetase/guanido kinase | 1 | 226 | 5.1E-104 |
| 2 | g8960.t22 | PANTHER | PTHR11547 | ARGININE OR CREATINE KINASE | 2 | 224 | 5.2E-103 |
| 3 | g8960.t22 | PANTHER | PTHR11547:SF20 | ARGININE KINASE | 2 | 224 | 5.2E-103 |
| 1 | g8960.t22 | Pfam | PF00217 | ATP:guanido phosphotransferase, C-terminal catalytic domain | 17 | 225 | 4.9E-87 |
| 5 | g8960.t22 | ProSitePatterns | PS00112 | Phosphagen kinase active site signature. | 141 | 147 | - |
| 7 | g8960.t22 | ProSiteProfiles | PS51510 | Phosphagen kinase C-terminal domain profile. | 1 | 226 | 83.055 |
| 4 | g8960.t22 | SUPERFAMILY | SSF55931 | Glutamine synthetase/guanido kinase | 2 | 225 | 3.64E-93 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0046314 | phosphocreatine biosynthetic process | BP |
| GO:0016301 | kinase activity | MF |
| GO:0004111 | creatine kinase activity | MF |
| GO:0003824 | catalytic activity | MF |
| GO:0016772 | transferase activity, transferring phosphorus-containing groups | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
