General information for g8960.t30
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Arginine kinase.
Parent gene
- The parent gene g8960 is located on chr_2: 34214230..34214913 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8960 | g8960.t30 | isoform | g8960.t30 | 34213004 | 34214277 |
| chr_2 | g8960 | g8960.t30 | exon | g8960.t30.exon1 | 34213004 | 34213280 |
| chr_2 | g8960 | g8960.t30 | TSS | g8960.t30 | 34213005 | 34213005 |
| chr_2 | g8960 | g8960.t30 | cds | g8960.t30.CDS1 | 34213019 | 34213280 |
| chr_2 | g8960 | g8960.t30 | exon | g8960.t30.exon2 | 34213336 | 34213874 |
| chr_2 | g8960 | g8960.t30 | cds | g8960.t30.CDS2 | 34213336 | 34213874 |
| chr_2 | g8960 | g8960.t30 | exon | g8960.t30.exon3 | 34213936 | 34214277 |
| chr_2 | g8960 | g8960.t30 | cds | g8960.t30.CDS3 | 34213936 | 34214166 |
| chr_2 | g8960 | g8960.t30 | TTS | g8960.t30 | 34214625 | 34214625 |
Sequences
>g8960.t30 Gene=g8960 Length=1158
AGTAACAAATCCGAAATGGTTGACGCTGCAGTACTTGAGAAATTGGAATCAGGCTTTGCC
AAGCTCCAAGCCTCTGACTCGAAATCATTGCTTAAAAAGTATTTGACCAAGGAAGTCTTT
GATAATCTTAAGAACAAGAAGACCCCAACATTTGGTTCAACTTTGCTTGATGTCATCCAA
TCTGGTTTAGAAAACCACGACTCTGGCGTCGGAATTTACGCACCAGATGCCGAGGCTTAC
ACAACTTTCGCTGAATTGTTTGACCCAATCATTGAAGACTACCATGGTGGATTCAAGAAG
ACCGACAAGCATCCAGCAAAGGACTTTGGTGATGTAAATGCATTTGGAGATTTGGATCCA
ACTGGTGAATTCGTTGTCTCAACTCGTGTCCGTTGCGGTCGCTCAATGGAAGGATATCCA
TTCAATCCATGCTTGACTGAAGCACAATACAAAGAAATGGAACAAAAGGTTTCAACCACA
TTGTCAGGCTTGGAAGGTGAATTGAAGGGCAAATTCTACCCATTGACTGGCATGGACAAA
GCTACTCAACAACAACTCATCGATGACCATTTCTTGTTCAAAGAAGGTGATCGCTTCTTG
CAAGCTGCCAATGCTTGCCGCTATTGGCCATCAGGTCGCGGTATCTACCACAATGATGCC
AAGACTTTCCTTGTCTGGTGCAATGAAGAAGATCACTTGCGTATCATTTCAATGCAAATG
GGTGGCAATCTTGGTGAAGTTTTCCGTCGTTTGACAACTGCCGTTAATGACATTGAGAAG
CGTATTCCATTCAGCCACAACGACCGCTTGGGATTCCTCACATTCTGCCCAACCAACTTG
GGAACAACCATCCGTGCATCAGTTCACATTAAGGTTCCAAAATTGGCTGCCAACAAAGCT
CGTCTTGAGGAAGTTGCTTCCAAATACAATTTGCAAGTTCGTGGCACACGCGGAGAACAT
ACAGAGGCTGAAGGTGGCATTTATGATATTTCAAACAAGAGACGTATGGGCTTGACTGAA
TTCCAAGCTGTCAAGGAGGTAAAATGATAAATTAAACATTTAAAAAGTTTTCATGCAATT
TATTAATTTTTTTATTCTTATTATTCACAGATGTACGATGGAATTACTGAAATCATCAAG
ATTGAAAAGTCATTGTAA
>g8960.t30 Gene=g8960 Length=343
MVDAAVLEKLESGFAKLQASDSKSLLKKYLTKEVFDNLKNKKTPTFGSTLLDVIQSGLEN
HDSGVGIYAPDAEAYTTFAELFDPIIEDYHGGFKKTDKHPAKDFGDVNAFGDLDPTGEFV
VSTRVRCGRSMEGYPFNPCLTEAQYKEMEQKVSTTLSGLEGELKGKFYPLTGMDKATQQQ
LIDDHFLFKEGDRFLQAANACRYWPSGRGIYHNDAKTFLVWCNEEDHLRIISMQMGGNLG
EVFRRLTTAVNDIEKRIPFSHNDRLGFLTFCPTNLGTTIRASVHIKVPKLAANKARLEEV
ASKYNLQVRGTRGEHTEAEGGIYDISNKRRMGLTEFQAVKEVK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g8960.t30 | CDD | cd07932 | arginine_kinase_like | 9 | 343 | 0.0 |
| 9 | g8960.t30 | Gene3D | G3DSA:1.10.135.10 | Transferase Creatine Kinase | 1 | 95 | 2.5E-38 |
| 8 | g8960.t30 | Gene3D | G3DSA:3.30.590.10 | Glutamine synthetase/guanido kinase | 98 | 343 | 5.0E-115 |
| 3 | g8960.t30 | PANTHER | PTHR11547:SF38 | ARGININE KINASE-RELATED | 8 | 342 | 9.2E-149 |
| 4 | g8960.t30 | PANTHER | PTHR11547 | ARGININE OR CREATINE KINASE | 8 | 342 | 9.2E-149 |
| 1 | g8960.t30 | Pfam | PF02807 | ATP:guanido phosphotransferase, N-terminal domain | 20 | 87 | 6.6E-30 |
| 2 | g8960.t30 | Pfam | PF00217 | ATP:guanido phosphotransferase, C-terminal catalytic domain | 147 | 342 | 8.6E-81 |
| 7 | g8960.t30 | ProSitePatterns | PS00112 | Phosphagen kinase active site signature. | 271 | 277 | - |
| 11 | g8960.t30 | ProSiteProfiles | PS51509 | Phosphagen kinase N-terminal domain profile. | 6 | 91 | 26.463 |
| 10 | g8960.t30 | ProSiteProfiles | PS51510 | Phosphagen kinase C-terminal domain profile. | 119 | 343 | 83.769 |
| 5 | g8960.t30 | SUPERFAMILY | SSF48034 | Guanido kinase N-terminal domain | 18 | 100 | 2.88E-33 |
| 6 | g8960.t30 | SUPERFAMILY | SSF55931 | Glutamine synthetase/guanido kinase | 97 | 342 | 1.01E-99 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0046314 | phosphocreatine biosynthetic process | BP |
| GO:0016301 | kinase activity | MF |
| GO:0004111 | creatine kinase activity | MF |
| GO:0003824 | catalytic activity | MF |
| GO:0016772 | transferase activity, transferring phosphorus-containing groups | MF |
KEGG
Orthology
- This transcript has been annotated with K00934 (arginine kinase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed