General information for g8960.t32
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Arginine kinase.
Parent gene
- The parent gene g8960 is located on chr_2: 34214230..34214913 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8960 | g8960.t32 | TSS | g8960.t32 | 34213005 | 34213005 |
| chr_2 | g8960 | g8960.t32 | isoform | g8960.t32 | 34213010 | 34214613 |
| chr_2 | g8960 | g8960.t32 | exon | g8960.t32.exon1 | 34213010 | 34213280 |
| chr_2 | g8960 | g8960.t32 | cds | g8960.t32.CDS1 | 34213019 | 34213280 |
| chr_2 | g8960 | g8960.t32 | exon | g8960.t32.exon2 | 34213336 | 34213874 |
| chr_2 | g8960 | g8960.t32 | cds | g8960.t32.CDS2 | 34213336 | 34213874 |
| chr_2 | g8960 | g8960.t32 | exon | g8960.t32.exon3 | 34213947 | 34214157 |
| chr_2 | g8960 | g8960.t32 | cds | g8960.t32.CDS3 | 34213947 | 34213991 |
| chr_2 | g8960 | g8960.t32 | exon | g8960.t32.exon4 | 34214230 | 34214613 |
| chr_2 | g8960 | g8960.t32 | TTS | g8960.t32 | 34214625 | 34214625 |
Sequences
>g8960.t32 Gene=g8960 Length=1405
AAATCCGAAATGGTTGACGCTGCAGTACTTGAGAAATTGGAATCAGGCTTTGCCAAGCTC
CAAGCCTCTGACTCGAAATCATTGCTTAAAAAGTATTTGACCAAGGAAGTCTTTGATAAT
CTTAAGAACAAGAAGACCCCAACATTTGGTTCAACTTTGCTTGATGTCATCCAATCTGGT
TTAGAAAACCACGACTCTGGCGTCGGAATTTACGCACCAGATGCCGAGGCTTACACAACT
TTCGCTGAATTGTTTGACCCAATCATTGAAGACTACCATGGTGGATTCAAGAAGACCGAC
AAGCATCCAGCAAAGGACTTTGGTGATGTAAATGCATTTGGAGATTTGGATCCAACTGGT
GAATTCGTTGTCTCAACTCGTGTCCGTTGCGGTCGCTCAATGGAAGGATATCCATTCAAT
CCATGCTTGACTGAAGCACAATACAAAGAAATGGAACAAAAGGTTTCAACCACATTGTCA
GGCTTGGAAGGTGAATTGAAGGGCAAATTCTACCCATTGACTGGCATGGACAAAGCTACT
CAACAACAACTCATCGATGACCATTTCTTGTTCAAAGAAGGTGATCGCTTCTTGCAAGCT
GCCAATGCTTGCCGCTATTGGCCATCAGGTCGCGGTATCTACCACAATGATGCCAAGACT
TTCCTTGTCTGGTGCAATGAAGAAGATCACTTGCGTATCATTTCAATGCAAATGGGTGGC
AATCTTGGTGAAGTTTTCCGTCGTTTGACAACTGCCGTTAATGACATTGAGAAGCGTATT
CCATTCAGCCACAACGACCGCTTGGGATTCCCCAACCAACTTGGGAACAACCATCCGTGC
ATCAGTTCACATTAAGGTTCCAAAATTGGCTGCCAACAAAGCTCGTCTTGAGGAAGTTGC
TTCCAAATACAATTTGCAAGTTCGTGGCACACGCGGAGAACATACAGAGGCTGAAGGTGG
CATTTATGATATTTCAAACAAGAGACGTATGGGCTTGACTGAATTCCAAGCTGTCAAGGA
GATGTACGATGGAATTACTGAAATCATCAAGATTGAAAAGTCATTGTAAAGTGTGTAAAA
GCAAAAACAAAACAACCCAACTTAAAATTGTCATCTATTTTCAAAATTATACATTAATAT
AAAAAACACATATACACACATGAATTAACAACAAAACTCAATCGTCATTTGCAATGGCGA
TCAAAGATTTGGAAATCTTTCATCAGTGATCTTTATTCTTGTTTAATTTAATTGATAAAA
AAATGTGTCCTATTCTCTCCATCTCAGTATAAGTTTGATGTGATTGATCACAAGCATTAA
GAATATTTTGGAATAAAATGTGAATTTAACATCTATTACCACATACAGCACATGCAAACA
AGAAGTTAATTAAATAAAAACCGTA
>g8960.t32 Gene=g8960 Length=281
MVDAAVLEKLESGFAKLQASDSKSLLKKYLTKEVFDNLKNKKTPTFGSTLLDVIQSGLEN
HDSGVGIYAPDAEAYTTFAELFDPIIEDYHGGFKKTDKHPAKDFGDVNAFGDLDPTGEFV
VSTRVRCGRSMEGYPFNPCLTEAQYKEMEQKVSTTLSGLEGELKGKFYPLTGMDKATQQQ
LIDDHFLFKEGDRFLQAANACRYWPSGRGIYHNDAKTFLVWCNEEDHLRIISMQMGGNLG
EVFRRLTTAVNDIEKRIPFSHNDRLGFPNQLGNNHPCISSH
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g8960.t32 | Gene3D | G3DSA:1.10.135.10 | Transferase Creatine Kinase | 1 | 95 | 0.000 |
| 7 | g8960.t32 | Gene3D | G3DSA:3.30.590.10 | Glutamine synthetase/guanido kinase | 98 | 274 | 0.000 |
| 3 | g8960.t32 | PANTHER | PTHR11547:SF38 | ARGININE KINASE-RELATED | 8 | 267 | 0.000 |
| 4 | g8960.t32 | PANTHER | PTHR11547 | ARGININE OR CREATINE KINASE | 8 | 267 | 0.000 |
| 1 | g8960.t32 | Pfam | PF02807 | ATP:guanido phosphotransferase, N-terminal domain | 20 | 87 | 0.000 |
| 2 | g8960.t32 | Pfam | PF00217 | ATP:guanido phosphotransferase, C-terminal catalytic domain | 147 | 267 | 0.000 |
| 10 | g8960.t32 | ProSiteProfiles | PS51509 | Phosphagen kinase N-terminal domain profile. | 6 | 91 | 26.463 |
| 9 | g8960.t32 | ProSiteProfiles | PS51510 | Phosphagen kinase C-terminal domain profile. | 119 | 281 | 47.410 |
| 5 | g8960.t32 | SUPERFAMILY | SSF48034 | Guanido kinase N-terminal domain | 18 | 100 | 0.000 |
| 6 | g8960.t32 | SUPERFAMILY | SSF55931 | Glutamine synthetase/guanido kinase | 97 | 267 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0046314 | phosphocreatine biosynthetic process | BP |
| GO:0016301 | kinase activity | MF |
| GO:0004111 | creatine kinase activity | MF |
| GO:0003824 | catalytic activity | MF |
| GO:0016772 | transferase activity, transferring phosphorus-containing groups | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
