General information for g8965.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Fatty acyl-CoA reductase wat.
Parent gene
- The parent gene g8965 is located on chr_2: 34241536..34244010 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8965 | g8965.t1 | TTS | g8965.t1 | 34241368 | 34241368 |
| chr_2 | g8965 | g8965.t1 | isoform | g8965.t1 | 34241536 | 34244010 |
| chr_2 | g8965 | g8965.t1 | exon | g8965.t1.exon1 | 34241536 | 34241777 |
| chr_2 | g8965 | g8965.t1 | cds | g8965.t1.CDS1 | 34241536 | 34241777 |
| chr_2 | g8965 | g8965.t1 | exon | g8965.t1.exon2 | 34242050 | 34242117 |
| chr_2 | g8965 | g8965.t1 | cds | g8965.t1.CDS2 | 34242050 | 34242117 |
| chr_2 | g8965 | g8965.t1 | exon | g8965.t1.exon3 | 34242281 | 34242388 |
| chr_2 | g8965 | g8965.t1 | cds | g8965.t1.CDS3 | 34242281 | 34242388 |
| chr_2 | g8965 | g8965.t1 | exon | g8965.t1.exon4 | 34242446 | 34242631 |
| chr_2 | g8965 | g8965.t1 | cds | g8965.t1.CDS4 | 34242446 | 34242631 |
| chr_2 | g8965 | g8965.t1 | exon | g8965.t1.exon5 | 34242692 | 34242838 |
| chr_2 | g8965 | g8965.t1 | cds | g8965.t1.CDS5 | 34242692 | 34242838 |
| chr_2 | g8965 | g8965.t1 | exon | g8965.t1.exon6 | 34242916 | 34243228 |
| chr_2 | g8965 | g8965.t1 | cds | g8965.t1.CDS6 | 34242916 | 34243228 |
| chr_2 | g8965 | g8965.t1 | exon | g8965.t1.exon7 | 34243285 | 34243374 |
| chr_2 | g8965 | g8965.t1 | cds | g8965.t1.CDS7 | 34243285 | 34243374 |
| chr_2 | g8965 | g8965.t1 | exon | g8965.t1.exon8 | 34243427 | 34243733 |
| chr_2 | g8965 | g8965.t1 | cds | g8965.t1.CDS8 | 34243427 | 34243733 |
| chr_2 | g8965 | g8965.t1 | exon | g8965.t1.exon9 | 34243807 | 34244010 |
| chr_2 | g8965 | g8965.t1 | cds | g8965.t1.CDS9 | 34243807 | 34244010 |
| chr_2 | g8965 | g8965.t1 | TSS | g8965.t1 | 34244046 | 34244046 |
Sequences
>g8965.t1 Gene=g8965 Length=1665
ATGGAAACACCTGAAATTATTGATTCTCCAATTGCTGATTTCTTTAAAAATAAAGTTGTA
TTCATAACAGGAGGTTTAGGATTTGTAGGAAAGTTATTGATTGAAAAATTATTACGATAT
GACGTGAAGAAAATTTATATTATGGCGCGTTCAAAGAAAGGAAAAACGATAGAAGAAAGA
TTGGAAGCATTATGTAATGAGCCTATTTTTATAAATTTAGCAAAGGATAAAGAGCGACAT
TTTAGTAAATTGAAGGTAATTTATGGAGACTTGCAGAAACTTCAATTAGGAATGGCGCCA
ATTGATGAACGGTTGTTAATTGATGAAGTCGAGATTTTTTATCATTGTGCTGCGGATGTG
AGATTTGATGAAAATTTAAAAGAGGCCATTGAAACGAATGTTCGTGGCACTCGAGAAATA
ATAAAACTTGCTCAACGACTTAAGACACTTAATGTTTTTGTTTATGTTTCAACAGCATTT
TGTACTCCATCATTTAATGTTAAAGAAAAATTTTATGACTCAACAATTGATCCAAATATG
ATGATAAAATATGTTGAAAATCTCAAAAGTCAAGAAGATATTGACAAATTTGAAATTTTT
GCACGTCGATTAATAAGACCATTTCCTAATACTTACACATTTACAAAATCACTAGCAGAG
CAAATGATACGAAAATATGCAGGAAATCTTAATTGTGCGATTATAAGGCCAGCAATAATA
ACTGTCACTTATGATGAGCCAATCGAAGGTTTTGCTGACAACATTTATGGCCTAAATGGA
GTGCTTGCTGGTGCTGGTGCTGGAGTTTTAAGAATAGTTTGCATTCGAAATGATTTTAAA
GCGAATATCGTTCCTGCAGATTATACAATTAATCTCATTATGGCAATTTCATTGTACACG
ATGACACTCGAAAATAATTTCAATTTCATTCGATGCACTGAAACTGAAAATAATCGAGTG
CAACTAGATGATGATGATGATGATGAGTTTCATTTGAATGAAGTCAACGATGATAAAATA
AAAGTCTATAATTTTAGCTCGAGTGATGAAAATCAAGTCACATGGGGCGATGTGAAGCGG
ATAACGACATCACTTGGACATAAAAATCCAATCAAGCGTCATTTATGGATCATCACTTAC
AATACTACACCATATCGATTGATTGCAAGAATTTTGAATATTTTTTATCATTTTATACCT
GCACTCTTTTTTGATTTAATTTTAATATTCAGAAAGAAACAACCAAAACTTTTAAAACTT
TACCGTAAGGTCAACAAGTTCACAGAAGTTATCGAGTTCTTCACAAACAACCAATGGCAT
TTTGATGACGATAATGTAATTCGTTTGTGGAATACAATGACGCCACATGATAAAAAGCTG
TTCAAATTCAATCTCTGGTCAATTGAGTGGCATCACTTTTTTGAACTTTATGTAAAGGGA
ATAAGGAAATATATTTATAAAGAAGATGAAGACACATTGGAAGTCGCACGAAAACGTTAT
CGACGTATAACAATAATACATTATTTAATGCTTTTTGCAATATGCGCATCATTATTTTAT
GGTGCATATAAAATTTTGATGCTTTATGTCATATCACATGATTTAATAGACAATTTTATC
CTTCGCACGAGATTCACGTATCTAAAATATGATGATATGGAGTAA
>g8965.t1 Gene=g8965 Length=554
METPEIIDSPIADFFKNKVVFITGGLGFVGKLLIEKLLRYDVKKIYIMARSKKGKTIEER
LEALCNEPIFINLAKDKERHFSKLKVIYGDLQKLQLGMAPIDERLLIDEVEIFYHCAADV
RFDENLKEAIETNVRGTREIIKLAQRLKTLNVFVYVSTAFCTPSFNVKEKFYDSTIDPNM
MIKYVENLKSQEDIDKFEIFARRLIRPFPNTYTFTKSLAEQMIRKYAGNLNCAIIRPAII
TVTYDEPIEGFADNIYGLNGVLAGAGAGVLRIVCIRNDFKANIVPADYTINLIMAISLYT
MTLENNFNFIRCTETENNRVQLDDDDDDEFHLNEVNDDKIKVYNFSSSDENQVTWGDVKR
ITTSLGHKNPIKRHLWIITYNTTPYRLIARILNIFYHFIPALFFDLILIFRKKQPKLLKL
YRKVNKFTEVIEFFTNNQWHFDDDNVIRLWNTMTPHDKKLFKFNLWSIEWHHFFELYVKG
IRKYIYKEDEDTLEVARKRYRRITIIHYLMLFAICASLFYGAYKILMLYVISHDLIDNFI
LRTRFTYLKYDDME
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g8965.t1 | CDD | cd05236 | FAR-N_SDR_e | 18 | 370 | 3.47382E-97 |
| 14 | g8965.t1 | CDD | cd09071 | FAR_C | 395 | 485 | 7.50382E-36 |
| 3 | g8965.t1 | PANTHER | PTHR11011:SF60 | FATTY ACYL-COA REDUCTASE 2 | 9 | 517 | 4.0E-147 |
| 4 | g8965.t1 | PANTHER | PTHR11011 | MALE STERILITY PROTEIN 2-RELATED | 9 | 517 | 4.0E-147 |
| 1 | g8965.t1 | Pfam | PF07993 | Male sterility protein | 22 | 293 | 9.5E-64 |
| 2 | g8965.t1 | Pfam | PF03015 | Male sterility protein | 396 | 488 | 6.1E-29 |
| 9 | g8965.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 386 | - |
| 12 | g8965.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 387 | 410 | - |
| 8 | g8965.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 411 | 504 | - |
| 11 | g8965.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 505 | 531 | - |
| 10 | g8965.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 532 | 554 | - |
| 7 | g8965.t1 | SUPERFAMILY | SSF51735 | NAD(P)-binding Rossmann-fold domains | 12 | 289 | 9.02E-31 |
| 5 | g8965.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 388 | 410 | - |
| 6 | g8965.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 509 | 531 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0080019 | fatty-acyl-CoA reductase (alcohol-forming) activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed