General information for g921.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Chymotrypsin-1.
Parent gene
- The parent gene g921 is located on chr_3: 6866430..6867884 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g921 | g921.t1 | TSS | g921.t1 | 6865818 | 6865818 |
| chr_3 | g921 | g921.t1 | isoform | g921.t1 | 6865841 | 6866908 |
| chr_3 | g921 | g921.t1 | exon | g921.t1.exon1 | 6865841 | 6865895 |
| chr_3 | g921 | g921.t1 | cds | g921.t1.CDS1 | 6865841 | 6865895 |
| chr_3 | g921 | g921.t1 | exon | g921.t1.exon2 | 6865954 | 6866007 |
| chr_3 | g921 | g921.t1 | cds | g921.t1.CDS2 | 6865954 | 6866007 |
| chr_3 | g921 | g921.t1 | exon | g921.t1.exon3 | 6866074 | 6866160 |
| chr_3 | g921 | g921.t1 | cds | g921.t1.CDS3 | 6866074 | 6866160 |
| chr_3 | g921 | g921.t1 | exon | g921.t1.exon4 | 6866224 | 6866365 |
| chr_3 | g921 | g921.t1 | cds | g921.t1.CDS4 | 6866224 | 6866365 |
| chr_3 | g921 | g921.t1 | exon | g921.t1.exon5 | 6866419 | 6866782 |
| chr_3 | g921 | g921.t1 | cds | g921.t1.CDS5 | 6866419 | 6866782 |
| chr_3 | g921 | g921.t1 | exon | g921.t1.exon6 | 6866846 | 6866908 |
| chr_3 | g921 | g921.t1 | cds | g921.t1.CDS6 | 6866846 | 6866908 |
| chr_3 | g921 | g921.t1 | TTS | g921.t1 | 6866992 | 6866992 |
Sequences
>g921.t1 Gene=g921 Length=765
ATGATTCGTCAAGTGCTACTTTTTGCTTTGTTTGTTTCTGTGGCTTTTGCTGCACCACAA
AATAGAGAAAGAAGAATTGTTGGTGGAGTTGATAATGTCTTGGGTGAAATACCATGGGCT
GTAAGCTTAAGAACAATAGCAGGACTTCATTTTTGTACAGGATCAGTTCTCAATAATTGG
TATGTTATCACATCAGCTCATTGCATCGATGGACGAGGAGCATCAACAATTCATTTAGTT
TTGGGTAGAATCACACTTGATGGATTAACTGGAATGAATCGTCAAGGTTTTAGAGTTATT
ATTCATCCTGCATATGATGTTTCAACTCAAAATGCTGATGTTGGTTTAATTCAAGTATCA
GTAATAATAACATTCAATGCAAATGTGCAAGCAATTTCATTAGGTGATACAATGATTGGA
ACTGGAGTTTCTGCAAGAGTGAGTGGTTGGGGTTCAACAGAACAAGACTTTGGACCAACT
TCGAATAATCTCAGATCACTTGATACAACAACACTTTCATTAAATGAATGTCAAGATAGA
CATACATGGTGGAATGCTGAAAGAATAAATATGAACCATCTCTGTACAAATAATGGAGTT
AATGAAGGTTTCTGTATGACAGGAGCTGGTGGATCTCTTGTCAGTAATTCACAATTAATC
GGTGTCGCTTCATGGAATGTACCTTGTGCTTTAGGCTATCCCGATGTTTATGTGAGAATA
TCAACATTCAGAACTTGGATAATGTCACAAATCGGTTCTCAATAA
>g921.t1 Gene=g921 Length=254
MIRQVLLFALFVSVAFAAPQNRERRIVGGVDNVLGEIPWAVSLRTIAGLHFCTGSVLNNW
YVITSAHCIDGRGASTIHLVLGRITLDGLTGMNRQGFRVIIHPAYDVSTQNADVGLIQVS
VIITFNANVQAISLGDTMIGTGVSARVSGWGSTEQDFGPTSNNLRSLDTTTLSLNECQDR
HTWWNAERINMNHLCTNNGVNEGFCMTGAGGSLVSNSQLIGVASWNVPCALGYPDVYVRI
STFRTWIMSQIGSQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g921.t1 | CDD | cd00190 | Tryp_SPc | 26 | 250 | 9.99198E-52 |
| 7 | g921.t1 | Gene3D | G3DSA:2.40.10.10 | - | 18 | 252 | 2.4E-47 |
| 2 | g921.t1 | PANTHER | PTHR24276 | POLYSERASE-RELATED | 6 | 251 | 1.8E-49 |
| 3 | g921.t1 | PANTHER | PTHR24276:SF78 | AT20289P-RELATED | 6 | 251 | 1.8E-49 |
| 1 | g921.t1 | Pfam | PF00089 | Trypsin | 26 | 247 | 8.0E-40 |
| 9 | g921.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 17 | - |
| 10 | g921.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 4 | - |
| 11 | g921.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 5 | 12 | - |
| 12 | g921.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 13 | 17 | - |
| 8 | g921.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 18 | 254 | - |
| 16 | g921.t1 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 26 | 252 | 24.971 |
| 15 | g921.t1 | SMART | SM00020 | trypsin_2 | 25 | 247 | 3.8E-38 |
| 4 | g921.t1 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 6 | 252 | 5.54E-56 |
| 6 | g921.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 17 | - |
| 14 | g921.t1 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 17 | - |
| 5 | g921.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 17 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed